Enzymatic Synthesis of Dipeptide Derivatives Containing Noncoded Amino Acids in Organic Solvents

A series of dipeptide derivatives containing non-coded amino acids, N-Boc-4-X-Phe-Ala-NHNHPh (X = Cl, Br, I, NO2), were synthesized by using thermoase in organic solvents. The physical data were consistent with the same samples prepared by 3-(diethoxyphosphoryloxy)-1, 2,3-benzotriazin-4 (3H)-one (DEPBT). Influence of different substituted groups of the noncoded amino acids and different organic solvents on the enzymatic peptide synthesis was studied.

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