Using VAAST to Identify Disease‐Associated Variants in Next‐Generation Sequencing Data

The VAAST pipeline is specifically designed to identify disease‐associated alleles in next‐generation sequencing data. In the protocols presented in this paper, we outline the best practices for variant prioritization using VAAST. Examples and test data are provided for case‐control, small pedigree, and large pedigree analyses. These protocols will teach users the fundamentals of VAAST, VAAST 2.0, and pVAAST analyses. Curr. Protoc. Hum. Genet. 81:6.14.1‐6.14.25. © 2014 by John Wiley & Sons, Inc.

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