Histological Co-Localization of Iron in Aß Plaques of PS/APP Transgenic Mice

This study confirms the presence of iron, co-localized with Aβ plaques, in PS/APP mouse brain, using Perls‘ stain for Fe3+ supplemented by 3,3′-diaminobenzidine (DAB) and Aβ immunohistochemistry in histological brains sections fixed with formalin or methacarn. In this study, the fixation process and the slice thickness did not interfere with the Perls’ technique. The presence of iron in ß-amyloid plaques in PS/APP transgenic mice, a model of Alzheimer’s disease (AD) pathology, may explain previous reports of reductions of transverse relaxation time (T2) in MRI studies and represent the source of the intrinsic Aß plaque MR contrast in this model.

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