The use of immunofluorescence and immunoperoxidase staining in studying the pathogenesis of chicken anaemia agent in experimentally infected chickens.

Both the indirect immunofluorescence test and the avidin-biotin method of immunoperoxidase staining were reliable techniques for the demonstration of chicken anaemia agent antigen in frozen or Bouin's-fixed, paraffin-embedded tissue sections. The main target cells of infection were thymic lymphoblasts in the outer cortex of the thymus and haematopoietic stem cells in the bone marrow.

[1]  S. Hayashi,et al.  Ultrastructure of the thymus in chicks inoculated with chicken anaemia agent (MSB1-TK5803 strain). , 1989, Avian pathology : journal of the W.V.P.A.

[2]  T. Nunoya,et al.  Enhanced pathogenicity of chicken anemia agent by infectious bursal disease virus relative to the occurrence of Marek's disease vaccination breaks. , 1989, Nihon juigaku zasshi. The Japanese journal of veterinary science.

[3]  T. Umemura,et al.  Ultrastructure of bone marrow in chicks inoculated with chicken anaemia agent (MSB1-TK5803 strain). , 1989, Avian pathology : journal of the W.V.P.A.

[4]  T. Umemura,et al.  Histopathology of chicks inoculated with chicken anaemia agent (MSB1-TK5803 strain). , 1989, Avian pathology : journal of the W.V.P.A.

[5]  G. Alm.  Lymphokines in the regulation of immune responses. , 1987, Veterinary immunology and immunopathology.

[6]  V. V. Bülow,et al.  Erhöhte Pathogenität des Erregers der aviären infektiösen Anämie bei Hühnerküken (CAA) bei simultaner Infektion mit Virus der Marekschen Krankheit (MDV), Bursitisvirus (IBDV) oder Reticuloendotheliosevirus (REV)* , 1986 .

[7]  L. Jönsson,et al.  Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin-embedded chicken tissues. , 1986 .

[8]  湯浅 襄.  Distribution of chicken anemia agent (CAA) and detection of neutralizing antibody in chicks experimentally inoculated with CAA. , 1983 .

[9]  T. Taniguchi,et al.  Chronological observations on hemato-pathological changes in chicks inoculated with chicken anemia agent. , 1983, National Institute of Animal Health quarterly.

[10]  T. Taniguchi,et al.  Hematopathological changes in dead and moribund chicks induced by chicken anemia agent. , 1982, National Institute of Animal Health quarterly.

[11]  S. Hsu,et al.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[12]  T. Taniguchi,et al.  Isolation and some characteristics of an agent inducing anemia in chicks. , 1979 .

[13]  D. Housman,et al.  Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro , 1978, The Journal of experimental medicine.