Cloning , expression , purification , and quantification of the 17 % N-terminal domain of apolipoprotein b100

Apolipoprotein B-100 (apo B) is the sole protein component of normal human low density lipoprotein (LDL). Elevated levels of LDL have been correlated with atherosclerosis and other coronary artery diseases. The large size of apo B (4536 aa) necessitates that it be studied in pieces corresponding to its structurally organized domains. The 17% N-terminal domain of apo B, simply B17, poses as one of these domains, having very specific structural characteristics. The current report describes a set of protocols for the cloning, expression, purification, and quantification of this important part of the protein.

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