Eicosapentaenoic acid-enriched phospholipids suppressed lipid accumulation by specific inhibition of lipid droplets-associated protein FSP27 in mice.

BACKGROUND Sea cucumber is rich sources of eicosapentaenoic acid in the form of phospholipids (EPA-PL), which is known to be efficacious in preventing obesity. However, few studies were focused on the role of EPA-PL in inhibiting lipid accumulation by lipid droplets (LDs). This study firstly investigated the effect of EPA-PL from sea cucumber on the formation of LDs as well as the underlying mechanism in C57BL/6J mice. The mice were randomly divided into two groups and treated for 8 weeks or 3, 7, 14 days: (1) high-sucrose diet (model group), (2) high-sucrose diet plus 2% EPA-PL (EPA-PL group). RESULTS Eight-week EPA-PL supplementation significantly reduced lipid accumulation and LDs size in liver and white adipose tissue (WAT), which was accompanied with the decreased expression of LDs-associated protein FSP27. A 3-day EPA-PL treatment suppressed the mRNA expression of Fsp27. The mRNA level of Fsp27 reached its 'normal level' after withdrawing EPA-PL for 7 days, suggesting that EPA-PL might serve as a rapid regulator of FSP27. Furthermore, EPA-PL increased the expression of lipolysis genes Hsl and Atgl accompanied by the regulation of Pparγ in WAT. CONCLUSIONS Dietary EPA-PL from sea cucumber Cucumaria frondosa protected against lipid accumulation through regulating LDs-associated protein FSP27, which might provide a novel evidence for the anti-obesity action of EPA-PL. This article is protected by copyright. All rights reserved.

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