Engineering and introduction of de novo disulphide bridges in organophosphorus hydrolase enzyme for thermostability improvement
暂无分享,去创建一个
[1] Frank Wien,et al. Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy , 2015, Proceedings of the National Academy of Sciences.
[2] Jianfang Li,et al. Contribution of Disulfide Bridges to the Thermostability of a Type A Feruloyl Esterase from Aspergillus usamii , 2015, PloS one.
[3] Eunsoo Hong,et al. Improved enantioselectivity of thermostable esterase from Archaeoglobus fulgidus toward (S)-ketoprofen ethyl ester by directed evolution and characterization of mutant esterases , 2015, Applied Microbiology and Biotechnology.
[4] R. Taheri,et al. Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major. , 2014, Asian Pacific journal of tropical medicine.
[5] N. Sheibani,et al. Inhibition study on insulin fibrillation and cytotoxicity by paclitaxel. , 2014, Journal of biochemistry.
[6] Gholamreza Farnoosh,et al. A Review on Engineering of Organophosphorus Hydrolase (OPH) Enzyme , 2014 .
[7] Kazi Zakia Sultana,et al. Protein disulfide engineering , 2014, FEBS letters.
[8] P. Zielenkiewicz,et al. Theoretical model explaining the relationship between the molecular mass and the activation energy of the enzyme revealed by a large-scale analysis of bioinformatics data. , 2013, Acta biochimica Polonica.
[9] Jian Tian,et al. Improving the thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 using a computationally aided method , 2013, Applied Microbiology and Biotechnology.
[10] J. Folch-Mallol,et al. Optimization of methyl parathion biodegradation and detoxification by cells in suspension or immobilized on tezontle expressing the opd gene , 2013, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.
[11] J. Joo,et al. Development of thermostable Candida antarctica lipase B through novel in silico design of disulfide bridge , 2012, Biotechnology and bioengineering.
[12] K. Khajeh,et al. Relationship between stability and flexibility in the most flexible region of Photinus pyralis luciferase. , 2012, Biochimica et biophysica acta.
[13] M. Koksharov,et al. Thermostabilization of Firefly Luciferases Using Genetic Engineering , 2012 .
[14] M. Nitschke,et al. Biodegradation of Pesticides , 2011 .
[15] K. Khajeh,et al. Improving the thermal stability of laccase from Bacillus sp. HR03 by site-directed mutagenesis at Asn143Pro , 2011 .
[16] Y. Su,et al. Improving the Thermostability of a Methyl Parathion Hydrolase by Adding the Ionic Bond on Protein Surface , 2011, Applied biochemistry and biotechnology.
[17] D. Ollis,et al. The organophosphate-degrading enzyme from Agrobacterium radiobacter displays mechanistic flexibility for catalysis. , 2010, The Biochemical journal.
[18] Ping Wang,et al. Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation , 2010, The FEBS journal.
[19] S. Hosseinkhani,et al. Design and introduction of a disulfide bridge in firefly luciferase: increase of thermostability and decrease of pH sensitivity , 2010, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.
[20] Jian Tian,et al. An intramolecular disulfide bond is required for the thermostability of methyl parathion hydrolase, OPHC2 , 2010, Applied Microbiology and Biotechnology.
[21] P. Pascutti,et al. The role of nonbonded interactions in the conformational dynamics of organophosphorous hydrolase adsorbed onto functionalized mesoporous silica surfaces. , 2010, The journal of physical chemistry. B.
[22] Himanshu Bhattacharjee,et al. Stability of Mesna in ReadyMed Infusion Devices , 2010, The Annals of pharmacotherapy.
[23] A. Mesecar,et al. Structure-based and random mutagenesis approaches increase the organophosphate-degrading activity of a phosphotriesterase homologue from Deinococcus radiodurans. , 2009, Journal of molecular biology.
[24] Shuangyan Han,et al. Enhancing thermostability of a Rhizomucor miehei lipase by engineering a disulfide bond and displaying on the yeast cell surface , 2009, Applied Microbiology and Biotechnology.
[25] C. D. Armstrong. Elucidating the chemical and thermal unfolding profiles of organophosphorus hydrolase and increasing its operational stability , 2009 .
[26] Sheetal Paliwal. Development of Enzyme-based Biosensors for the Detection of Organophosphate Neurotoxins , 2008 .
[27] B. Ranjbar,et al. Comparative studies of wild type Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase with three glyphosate-insensitive mutated forms: activity, stability and structural characterization. , 2008, Biochimica et biophysica acta.
[28] James E. Hall,et al. Transformation of plasmid DNA into E. coli using the heat shock method. , 2007, Journal of visualized experiments : JoVE.
[29] B. Ásgeirsson,et al. Engineered disulfide bonds increase active-site local stability and reduce catalytic activity of a cold-adapted alkaline phosphatase. , 2007, Biochimica et biophysica acta.
[30] K. Meng,et al. Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant , 2007, Journal of Industrial Microbiology & Biotechnology.
[31] D. Fournier,et al. The effect of engineered disulfide bonds on the stability of Drosophila melanogaster acetylcholinesterase , 2006, BMC Biochemistry.
[32] F. Raushel,et al. Detoxification of organophosphate nerve agents by bacterial phosphotriesterase. , 2005, Toxicology and applied pharmacology.
[33] T. S. Pinkerton. The recombinant expression and potential applications of bacterial organophosphate hydrolase in Zea mays L. , 2005 .
[34] Dieter Jahn,et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host , 2005, Nucleic Acids Res..
[35] Georges Feller,et al. Temperature adaptation of proteins: engineering mesophilic-like activity and stability in a cold-adapted alpha-amylase. , 2003, Journal of molecular biology.
[36] Alan A. Dombkowski,et al. Disulfide by DesignTM: a computational method for the rational design of disulfide bonds in proteins , 2003, Bioinform..
[37] Michael Zuker,et al. Mfold web server for nucleic acid folding and hybridization prediction , 2003, Nucleic Acids Res..
[38] J. Oakeshott,et al. Evolution of an organophosphate-degrading enzyme: a comparison of natural and directed evolution. , 2003, Protein engineering.
[39] R. Varadarajan,et al. Elucidation of factors responsible for enhanced thermal stability of proteins: a structural genomics based study. , 2002, Biochemistry.
[40] P. Callis,et al. Mechanisms of tryptophan fluorescence shifts in proteins. , 2001, Biophysical journal.
[41] K. Lai,et al. Rational design of organophosphorus hydrolase for altered substrate specificities. , 1999, Chemico-biological interactions.
[42] T. Kortemme,et al. Ionization-reactivity relationships for cysteine thiols in polypeptides. , 1998, Biochemistry.
[43] H. Ertan,et al. Stabilization of Escherichia coli penicillin G acylase against thermal inactivation by cross-linking with dextran dialdehyde polymers , 1997, Applied Microbiology and Biotechnology.
[44] L. Pearl,et al. Enzyme thermostability and thermoactivity. , 1996, Protein engineering.
[45] S. Harvey,et al. Cloning and expression of a gene encoding a bacterial enzyme for decontamination of organophosphorus nerve agents and nucleotide sequence of the enzyme , 1996, Applied and environmental microbiology.
[46] D. Santi,et al. Thermal stabilization of thymidylate synthase by engineering two disulfide bridges across the dimer interface. , 1994, Journal of molecular biology.
[47] R L Blakeley,et al. Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination. , 1979, Analytical biochemistry.
[48] A. Mohsenifar,et al. Enhancing organophosphorus hydrolase stability by immobilization on chitosan beads containing glutaraldehyde , 2015 .
[49] A. Fersht,et al. Highest paraoxonase turnover rate found in a bacterial phosphotriesterase variant. , 2011, Protein engineering, design & selection : PEDS.
[50] A. Grunden,et al. Hydrolysis of organophosphorus compounds by microbial enzymes , 2010, Applied Microbiology and Biotechnology.
[51] A. Aitken,et al. Estimation of Disulfide Bonds Using Ellman’s Reagent , 2009 .
[52] Ssang-Goo Cho,et al. Engineering a de novo internal disulfide bridge to improve the thermal stability of xylanase from Bacillus stearothermophilus No. 236. , 2007, Journal of biotechnology.