Semi‐Egg‐Like Heterogeneous Compartmentalization of Cells Controlled by Contact Angle Hysteresis

Precise control of liquid inside compartments is critically important in bioreactors, combinatorial analysis, and tissue engineering. A contact angle hysteresis (CAH)‐based strategy is demonstrated to construct a semi‐egg‐like hydrogel architecture, leading to spatial heterogeneous compartmentalization of cells. The semi‐egg‐like architecture is fabricated by successively capturing and gelling prehydrogel liquids using a substrate with controlled‐CAH pattern and ultralow‐CAH background. The controlled‐CAH pattern could capture liquid with tunable size, while ultralow‐CAH background prevents liquid sticking. It is envisioned that this CAH‐based strategy would be promising in designing functional surface for engineering complicated architectures of either biomedical or nonbiomedical systems.

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