Application of laser printing to mammalian cells

Abstract A laser-based transfer technique, termed matrix assisted pulsed laser evaporation direct write (MAPLE DW), has been modified to rapidly and accurately deposit mammalian cells in a non-contact manner. While this technology was originally developed for deposition of inorganic materials, it has shown the ability to transfer of a wide range of biological materials. Two types of mammalian cells, human osteosarcoma and rat cardiac cells were deposited into a biopolymer matrix via MAPLE DW. Current results show that it is possible to deposit cells in a stepwise manner and build cellular ‘stacks’ 50–100 μm tall. Furthermore, the technique is now capable of depositing cells with near single cell resolution. Post-transfer results of live/dead viability/cytotoxicity assays show that the cells are unaffected by the process with near 100% viability. The ability to build cellular structures in three dimensions and deposit small numbers of cells accurately has potential application to tissue engineering.

[1]  Vladimir Mironov,et al.  Organ printing: computer-aided jet-based 3D tissue engineering. , 2003, Trends in biotechnology.

[2]  Frank P. Incropera,et al.  Fundamentals of Heat and Mass Transfer , 1981 .

[3]  D. Krizman,et al.  Picoliter‐Scale Protein Microarrays by Laser Direct Write , 2002, Biotechnology progress.

[4]  B R Ringeisen,et al.  Generation of mesoscopic patterns of viable Escherichia coli by ambient laser transfer. , 2002, Biomaterials.

[5]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. , 2002, Tissue engineering.

[6]  R. Crystal,et al.  Formation of vascularized meniscal tissue by combining gene therapy with tissue engineering. , 2002, Tissue engineering.

[7]  R. A. McGill,et al.  The deposition, structure, pattern deposition, and activity of biomaterial thin-films by matrix-assisted pulsed-laser evaporation (MAPLE) and MAPLE direct write , 2001 .

[8]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[9]  Antonios G. Mikos,et al.  Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[10]  R. A. McGill,et al.  Laser transfer of biomaterials: Matrix-assisted pulsed laser evaporation (MAPLE) and MAPLE Direct Write , 2003 .

[11]  R A Good,et al.  Cellular engineering. , 1977, Clinical bulletin.

[12]  G. M. Hale,et al.  Optical Constants of Water in the 200-nm to 200-microm Wavelength Region. , 1973, Applied optics.