Super-resolution coherent anti-Stokes Raman scattering microscopy with photonic nanojets.

We demonstrate far-field super-resolution coherent anti-Stokes Raman scattering (CARS) microscopy by exciting the sample with photonic nanojets. The sub-diffraction photonic nanojets were formed on the surface of the sample by microspheres illuminated by laser beams, and images were acquired by a standard laser-scanning CARS microscope. When the laser beams were focused on the microspheres, the photonic nanojets determined the excitation volume instead of the diffraction-limited laser foci, leading to super-resolution. We imaged the sub-diffraction features of a Blu-ray disc using glass spheres with a refractive index of 1.46 and diameters in the 1-6 µm range. The microspheres provided a lateral magnification factor up to 5.0X and a lateral resolution of at least 200 nm at 796 nm laser wavelength, allowing us to resolve the features on the disc which were invisible under normal CARS imaging. The magnification factor depended on both the microsphere size and the focal plane position of the incident beams. To explain the magnification factor we performed theoretical simulations which showed excellent agreement with experimental results. This super-resolution technique could be very useful for the vibrational imaging of nano-scale objects on films and surfaces.

[1]  Y. S. Zhou,et al.  Contrast enhancement using silica microspheres in coherent anti-Stokes Raman spectroscopic imaging. , 2014, Optics express.

[2]  Michel Piché,et al.  Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy. , 2013, Optics letters.

[3]  Zengbo Wang,et al.  Overcoming the diffraction limit induced by microsphere optical nanoscopy , 2013 .

[4]  Wei Zheng,et al.  Radially polarized tip-enhanced near-field coherent anti-Stokes Raman scattering microscopy for vibrational nano-imaging , 2013 .

[5]  Paul Kumar Upputuri,et al.  Circularly polarized coherent anti-Stokes Raman scattering microscopy. , 2013, Optics letters.

[6]  Arash Darafsheh,et al.  Optical super-resolution by high-index liquid-immersed microspheres , 2012 .

[7]  S. Kawata,et al.  Tip-enhanced broadband CARS spectroscopy and imaging using a photonic crystal fiber based broadband light source† , 2012 .

[8]  Xu Liu,et al.  Microsphere based microscope with optical super-resolution capability , 2011 .

[9]  Zengbo Wang,et al.  Optical virtual imaging at 50 nm lateral resolution with a white-light nanoscope. , 2011, Nature communications.

[10]  Halina Rubinsztein-Dunlop,et al.  A method for achieving super-resolved widefield CARS microscopy. , 2010, Optics express.

[11]  Carsten Fallnich,et al.  A route to sub-diffraction-limited CARS Microscopy. , 2009, Optics express.

[12]  D. Palanker,et al.  On illumination schemes for wide-field CARS microscopy. , 2009, Optics express.

[13]  Hervé Rigneault,et al.  Three-dimensional subwavelength confinement of light with dielectric microspheres. , 2009, Optics express.

[14]  Vishnu Vardhan Krishnamachari,et al.  Interferometric switching of coherent anti-Stokes Raman scattering signals in microscopy. , 2009, Physical review. A, Atomic, molecular, and optical physics.

[15]  Ji-Xin Cheng,et al.  Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman scattering microscopy. , 2008, Optics express.

[16]  Din Ping Tsai,et al.  Characterization of nano recorded marks at different writing strategies on phase-change recording layer of optical disks. , 2006, Optics express.

[17]  Fuxi Gan,et al.  Organic materials for recordable blue laser optical storage , 2005, International Workshop on Information Data Storage and International Symposium on Optical Storage.

[18]  Riyi Shi,et al.  Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues. , 2005, Biophysical journal.

[19]  Allen Taflove,et al.  Optical analysis of nanoparticles via enhanced backscattering facilitated by 3-D photonic nanojets. , 2005, Optics express.

[20]  S. Kawata,et al.  Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging. , 2004, Physical review letters.

[21]  Allen Taflove,et al.  Photonic nanojet enhancement of backscattering of light by nanoparticles: a potential novel visible-light ultramicroscopy technique. , 2004, Optics express.

[22]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[23]  铃木晴之.  Optical disk and optical recording method , 2003 .

[24]  Richard D. Schaller,et al.  Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM) , 2002 .

[25]  Andreas Volkmer,et al.  Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy , 2002 .

[26]  Andreas Volkmer,et al.  Vibrational Imaging with High Sensitivity via Epidetected Coherent Anti-Stokes Raman Scattering Microscopy , 2001 .