Thermally induced changes of optical and vital parameters in human cancer cells

Minimally invasive laser-induced thermotherapy (LITT) presents an alternative method to conventional tumor therapeutically interventions, such as surgery, chemotherapy, radiotherapy or nuclear medicine. Optical tissue characteristics of tumor cells and their heat-induced changes are essential issues for controlling LITT progressions. Therefore, it is indispensable to exactly know the absorption coefficient μa, the scattering coefficient μs and the anisotropy factor g as well as their changes under rising temperatures in order to simulate the treatment parameters successfully. Optical parameters of two different cancer model tissues - breast cancer cells species MX1 and colon cancer cells species CX1 – were measured in the spectral range 400 – 1100 nm as well as in the temperature range 37 – 60°C. The absorption coefficient of both cell species was low throughout the spectral range analyzed, while μs of both species rose with increasing temperatures. The anisotropy factor g however dropped for both tissues with increasing temperatures. Light scatterings inside tissues proceeded continuously forward for all species tested. It was demonstrated that optical tissue properties undergo significant changes along with the vital status of the cells when the temperature increases.

[1]  J. L. Roti,et al.  Cellular responses to hyperthermia (40-46°C) : Cell killing and molecular events , 2008 .

[2]  C. Garrel,et al.  Free‐radical production triggered by hyperthermia contributes to heat stress‐induced cardioprotection in isolated rat hearts , 2002, British journal of pharmacology.

[3]  Jürgen Beuthan,et al.  Microscopical heat stress investigations under application of quantum dots. , 2005, Journal of biomedical optics.

[4]  V. Bagnato,et al.  Correlation between the photostability and photodynamic efficacy for different photosensitizers , 2006 .

[5]  I. Wilson,et al.  Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. , 2000, European journal of biochemistry.

[6]  Dosimetric investigations of laser-induced phase transition of MX1-cell membranes by use of quantum dots , 2006 .

[7]  O. Minet,et al.  Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress , 2010 .

[8]  P. Pinton,et al.  The versatility of mitochondrial calcium signals: from stimulation of cell metabolism to induction of cell death. , 2008, Biochimica et biophysica acta.

[9]  V. Knappe,et al.  Ex vivo and in vivo evaluation of laser‐induced thermotherapy for nodular thyroid disease , 2009, Lasers in surgery and medicine.

[10]  V. Baskov,et al.  Laser engineering of spine discs , 2009 .

[11]  Valery V. Tuchin,et al.  TiO2 nanoparticle enhanced photodynamic inhibition of pathogens , 2010 .

[12]  T Lenarz,et al.  In vitro measurement conditions for optical coherence tomography (OCT) , 2006, Acta oto-laryngologica.

[13]  Huajiang Wei,et al.  Thermal coagulation-induced changes of the optical properties of normal and adenomatous human colon tissues in vitro in the spectral range 400–1100 nm , 2008, Physics in medicine and biology.

[14]  S Andersson-Engels,et al.  Changes in spectral shape of tissue optical properties in conjunction with laser-induced thermotherapy. , 1998, Applied optics.

[15]  L. Berliner,et al.  Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle. , 2000, American journal of physiology. Cell physiology.

[16]  F Fobbe,et al.  Magnetic Resonance Imaging—Controlled Laser–Induced Interstitial Thermotherapy , 1994, Investigative radiology.

[17]  B. Goliaei,et al.  The role of heat shock protein 70 in the thermoresistance of prostate cancer cell line spheroids , 2004, FEBS letters.

[18]  Chuanshan Xu,et al.  LED-activated pheophorbide a induces cellular destruction of colon cancer cells , 2010 .

[19]  O. Minet,et al.  Changes in laser-induced fluorescence responses of 3T3 fibroblasts to repetitive thermal stress , 2009 .

[20]  G. Hahn,et al.  Stable heat‐resistant clones selected from wild‐type and surface variants of B‐16 melanoma , 1983, International journal of cancer.

[21]  J C Bischof,et al.  Dynamics of cell membrane permeability changes at supraphysiological temperatures. , 1995, Biophysical journal.

[22]  J. Čiapaitė,et al.  Acute Temperature Resistance Threshold in Heart Mitochondria: Febrile Temperature Activates Function but Exceeding It Collapses the Membrane Barrier , 2022 .

[23]  V. Cardile,et al.  Adaptive Responses to the Stress Induced by Hyperthermia or Hydrogen Peroxide in Human Fibroblasts , 2003, Experimental biology and medicine.

[24]  O. Minet,et al.  Calculations regarding cell metabolism stimulation using photons in the visible wavelength range , 2007 .

[25]  Olaf Minet,et al.  Fluorescence Imaging of Heat-Stress Induced Mitochondrial Long-Term Depolarization in Breast Cancer Cells , 2006, Journal of Fluorescence.

[26]  Anthony J McGoron,et al.  Combined effects of laser-ICG photothermotherapy and doxorubicin chemotherapy on ovarian cancer cells. , 2009, Journal of photochemistry and photobiology. B, Biology.

[27]  V. Bagnato,et al.  Nutritional stress enhances cell viability of odontoblast-like cells subjected to low level laser irradiation , 2010 .

[28]  Qingming Luo,et al.  Effects of dehydration on the optical properties of in vitro porcine liver , 2003, Lasers in surgery and medicine.

[29]  New non-invasive methods for the investigation of cerebral oxidative metabolism and haemodynamics in newborn infants. , 1991, Annals of medicine.

[30]  O. Minet,et al.  Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process , 2010 .

[31]  R. Chammas,et al.  Hyperthermia increases the metastatic potential of murine melanoma. , 1997, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[32]  K. Delman,et al.  The role of hyperthermia in optimizing tumor response to regional therapy , 2008, International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group.

[33]  G. Hahn,et al.  Effect of hyperthermia (45 degrees C) on calcium flux in Chinese hamster ovary HA-1 fibroblasts and its potential role in cytotoxicity and heat resistance. , 1987, Cancer research.

[34]  J Beuthan,et al.  The spatial variation of the refractive index in biological cells. , 1996, Physics in medicine and biology.

[35]  O. Minet,et al.  Fluorescence Imaging of Mitochondrial Long-Term Depolarization in Cancer Cells Exposed to Heat-Stress , 2009 .

[36]  Gerhard Müller,et al.  Optical properties of native and coagulated human liver tissue and liver metastases in the near infrared range , 1998, Lasers in surgery and medicine.

[37]  V. V. Lunin,et al.  Eye tissue structure and refraction alterations upon nondestructive laser action , 2006 .

[38]  Hidetake Imasato,et al.  A combination of techniques to evaluate photodynamic efficiency of photosensitizers , 2008 .

[39]  V. Pustovalov,et al.  Theoretical investigations of the processes of selective laser interaction with melanin granules in pigmented tissues for laser applications in medicine , 2006 .

[41]  Huabei Jiang,et al.  Multispectral diffuse optical tomography with absorption and scattering spectral constraints. , 2007, Applied optics.

[42]  R. Lee,et al.  The relative thermal stability of tissue macromolecules and cellular structure in burn injury. , 2005, Burns : journal of the International Society for Burn Injuries.