Quantitative expression of mRNA encoding BMP/SMAD signaling genes in the ovaries of Booroola carrier and non-carrier GMM sheep.

The GMM sheep is a carrier of Booroola fecundity (FecB) gene, which produces the twins and triplets in one lambing. The homozygous carrier GMM (FecBBB ), non-carrier GMM and Malpura (FecB++ ) ewes were synchronized by progesterone sponges and the plasma progesterone concentration was measured by RIA. The results showed that the progesterone concentration did not differ significantly (p>0.05) in homozygous carrier GMM (5.74±1.2ng/ml), non-carrier GMM (5.42±1.4ng/ml) and non-carrier Malpura ewes (5.67±1.5ng/ml). Further, quantitative expression of BMP factors/receptor and SMAD signaling genes were analyzed in the ovaries of sheep by qRT-PCR. The study showed that the expression of BMP2 was slightly higher (p>0.05) in carrier GMM than that of non-carrier GMM, but it was almost similar to Malpura ewes. Expression of BMP4 and BMP7 was significantly higher (p<0.001; p<0.05) in carrier GMM than that of non-carrier GMM and Malpura ewes. Although, BMP6 expression was higher (p>0.05) in carrier GMM than that of non-carrier GMM, but lower (p>0.05) than the Malpura ewes. Expression of BMP15 (p<0.05), GDF5 (p<0.01) and GDF9 (p<0.05) was significantly higher in carrier GMM than the non-carrier GMM ewes. Surprisingly, BMPR1B expression was significantly higher (p<0.001) in non-carrier GMM and Malpura than the carrier GMM ewes, while TGFβRI did not differ significantly (p>0.05) among both GMM genotypes. On the other hand, expression of BMPR1A (p>0.05) and BMPRII (p<0.05) was higher in carrier GMM than the non-carrier GMM, but significantly lower (p<0.001) than the Malpura ewes. It was interesting to note that the expression of SMAD1 (p>0.05), SMAD2 (p<0.001), SMAD3 (p<0.05), SMAD4 (p<0.001), SMAD5 (p<0.001), and SMAD8 (p<0.001) was lower in the carrier GMM than that of non-carrier GMM ewes. It is concluded that the FecB mutation alters the expression of BMPR1B and SMAD signaling genes in the ovaries of homozygous carrier GMM ewes. This article is protected by copyright. All rights reserved.

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