miR-22-5p and miR-29a-5p Are Reliable Reference Genes for Analyzing Extracellular Vesicle-Associated miRNAs in Adipose-Derived Mesenchymal Stem Cells and Are Stable under Inflammatory Priming Mimicking Osteoarthritis Condition

[1]  Hao Yang,et al.  Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis , 2018, Experimental and therapeutic medicine.

[2]  P. Soon-Shiong,et al.  Pro inflammatory stimuli enhance the immunosuppressive functions of adipose mesenchymal stem cells-derived exosomes , 2018, Scientific Reports.

[3]  M. Alcaraz,et al.  Extracellular vesicles: A new therapeutic strategy for joint conditions. , 2018, Biochemical pharmacology.

[4]  P. De Luca,et al.  Housekeeping Gene Stability in Human Mesenchymal Stem and Tendon Cells Exposed to Tenogenic Factors. , 2018, Tissue engineering. Part C, Methods.

[5]  A. Colombini,et al.  Validation of reference and identity-defining genes in human mesenchymal stem cells cultured under unrelated fetal bovine serum batches for basic science and clinical application , 2018, Stem Cell Reviews and Reports.

[6]  M. Alcaraz,et al.  Microvesicles from Human Adipose Tissue-Derived Mesenchymal Stem Cells as a New Protective Strategy in Osteoarthritic Chondrocytes , 2018, Cellular Physiology and Biochemistry.

[7]  E. P. González,et al.  Adipose tissue stem cells in regenerative medicine , 2018, Ecancermedicalscience.

[8]  S. Butcher,et al.  The life of U6 small nuclear RNA, from cradle to grave , 2018, RNA.

[9]  Graça Raposo,et al.  Shedding light on the cell biology of extracellular vesicles , 2018, Nature Reviews Molecular Cell Biology.

[10]  An Zhao,et al.  MicroRNAs in Serum Exosomes as Potential Biomarkers in Clear-cell Renal Cell Carcinoma. , 2016, European urology focus.

[11]  Tianfei Yang,et al.  Effective enrichment of urinary exosomes by polyethylene glycol for RNA detection , 2018 .

[12]  C. Jorgensen,et al.  Mesenchymal stem cells derived exosomes and microparticles protect cartilage and bone from degradation in osteoarthritis , 2017, Scientific Reports.

[13]  E. Marbán,et al.  A comprehensive method for identification of suitable reference genes in extracellular vesicles , 2017, Journal of extracellular vesicles.

[14]  P. Robbins,et al.  Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes , 2017, Scientific Reports.

[15]  Zhongdang Xiao,et al.  MicroRNA profiling analysis revealed different cellular senescence mechanisms in human mesenchymal stem cells derived from different origin. , 2017, Genomics.

[16]  S. Fan,et al.  Exosomes containing differential expression of microRNA and mRNA in osteosarcoma that can predict response to chemotherapy , 2017, Oncotarget.

[17]  D. Consonni,et al.  Plasmatic extracellular vesicle microRNAs in malignant pleural mesothelioma and asbestos-exposed subjects suggest a 2-miRNA signature as potential biomarker of disease , 2017, PloS one.

[18]  N. Rothwell,et al.  Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro , 2017, Stem Cell Research & Therapy.

[19]  Jing Wang,et al.  Isolation and Identification of miRNAs in exosomes derived from serum of colon cancer patients , 2017, Journal of Cancer.

[20]  Jinxi Wang,et al.  Role of MicroRNA in Osteoarthritis , 2017, Journal of arthritis.

[21]  L. Valdatta,et al.  Identification of reference genes for qPCR analysis during hASC long culture maintenance , 2017, PloS one.

[22]  R. Cancedda,et al.  Mesenchymal Stem Cell‐Derived Extracellular Vesicles as Mediators of Anti‐Inflammatory Effects: Endorsement of Macrophage Polarization , 2017, Stem cells translational medicine.

[23]  Toru Hayashi,et al.  Exosomal MicroRNA Transport from Salivary Mesenchyme Regulates Epithelial Progenitor Expansion during Organogenesis. , 2017, Developmental cell.

[24]  Xiaobing Fu,et al.  Mesenchymal stem cells-derived exosomal microRNAs contribute to wound inflammation , 2016, Science China Life Sciences.

[25]  J. Xie,et al.  MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis , 2016, Molecules and cells.

[26]  Yutian Li,et al.  MiRNA-Mediated Macrophage Polarization and its Potential Role in the Regulation of Inflammatory Response , 2016, Shock.

[27]  F. Piva,et al.  The choice of endogenous controls in exosomal microRNA assessments from biofluids , 2016, Tumor Biology.

[28]  S. Giovanelli,et al.  Angiogenic and anti-inflammatory properties of mesenchymal stem cells from cord blood: soluble factors and extracellular vesicles for cell regeneration. , 2016, European journal of cell biology.

[29]  Lingyan Wang,et al.  Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation , 2016, EBioMedicine.

[30]  Bo Xu,et al.  Identification of TLR2 and TLR4-induced microRNAs in human mesenchymal stem cells and their possible roles in regulating TLR signals , 2016, Molecular medicine reports.

[31]  D. Argyle,et al.  In vitro models for the study of osteoarthritis. , 2016, Veterinary journal.

[32]  N. Puig,et al.  MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry , 2016, Cell Communication and Signaling.

[33]  Klaus Pantel,et al.  Data Normalization Strategies for MicroRNA Quantification. , 2015, Clinical chemistry.

[34]  Xiaobing Fu,et al.  LPS-preconditioned mesenchymal stromal cells modify macrophage polarization for resolution of chronic inflammation via exosome-shuttled let-7b , 2015, Journal of Translational Medicine.

[35]  Yi Li,et al.  Assessment of endogenous reference gene suitability for serum exosomal microRNA expression analysis in liver carcinoma resection studies. , 2015, Molecular medicine reports.

[36]  Jaak Vilo,et al.  ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap , 2015, Nucleic Acids Res..

[37]  M. Klinker,et al.  Mesenchymal stem cells in the treatment of inflammatory and autoimmune diseases in experimental animal models. , 2015, World journal of stem cells.

[38]  R. Bellomo,et al.  Assessment of Cell-Cycle Arrest Biomarkers to Predict Early and Delayed Acute Kidney Injury , 2015, Disease markers.

[39]  Yi Li,et al.  Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma , 2015, Disease markers.

[40]  L. O’Driscoll,et al.  Biological properties of extracellular vesicles and their physiological functions , 2015, Journal of extracellular vesicles.

[41]  Verónica Álvarez,et al.  Immunomodulatory Potential of Human Adipose Mesenchymal Stem Cells Derived Exosomes on in vitro Stimulated T Cells , 2014, Front. Immunol..

[42]  M. Fini,et al.  Adipose-derived mesenchymal stem cells for cartilage tissue engineering: state-of-the-art in in vivo studies. , 2014, Journal of biomedical materials research. Part A.

[43]  F. Buttitta,et al.  Plasma exosome microRNA profiling unravels a new potential modulator of adiponectin pathway in diabetes: effect of glycemic control. , 2014, The Journal of clinical endocrinology and metabolism.

[44]  M. Moretti,et al.  Donor-matched mesenchymal stem cells from knee infrapatellar and subcutaneous adipose tissue of osteoarthritic donors display differential chondrogenic and osteogenic commitment. , 2014, European cells & materials.

[45]  M. Stiehler,et al.  Identification of suitable reference genes in bone marrow stromal cells from osteoarthritic donors. , 2013, Stem cell research.

[46]  T. Luedde,et al.  U6 is unsuitable for normalization of serum miRNA levels in patients with sepsis or liver fibrosis , 2013, Experimental & Molecular Medicine.

[47]  P. Rebulla,et al.  Differential microRNA signature of human mesenchymal stem cells from different sources reveals an "environmental-niche memory" for bone marrow stem cells. , 2013, Experimental cell research.

[48]  C. Manferdini,et al.  Adipose-derived mesenchymal stem cells exert antiinflammatory effects on chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin E2. , 2013, Arthritis and rheumatism.

[49]  S. Law,et al.  Mesenchymal stem cell and regenerative medicine: regeneration versus immunomodulatory challenges. , 2013, American journal of stem cells.

[50]  Paolo Rebulla,et al.  What is beyond a qRT-PCR study on mesenchymal stem cell differentiation properties: how to choose the most reliable housekeeping genes , 2013, Journal of cellular and molecular medicine.

[51]  H. Weinans,et al.  Mesenchymal stem cells secrete factors that inhibit inflammatory processes in short-term osteoarthritic synovium and cartilage explant culture. , 2012, Osteoarthritis and cartilage.

[52]  G. Muller.  Novel Tools for the Study of Cell Type-Specific Exosomes and Microvesicles , 2012 .

[53]  Baohong Zhang,et al.  miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs , 2012, Plant Molecular Biology.

[54]  C. Will,et al.  Spliceosome structure and function. , 2011, Cold Spring Harbor perspectives in biology.

[55]  W. Weimar,et al.  Inflammatory conditions affect gene expression and function of human adipose tissue‐derived mesenchymal stem cells , 2010, Clinical and experimental immunology.

[56]  V. Beneš,et al.  The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. , 2009, Clinical chemistry.

[57]  Manuel A. González,et al.  Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. , 2009, Gastroenterology.

[58]  W. Weimar,et al.  Human heart, spleen, and perirenal fat-derived mesenchymal stem cells have immunomodulatory capacities. , 2007, Stem cells and development.

[59]  R. Yañez,et al.  Adipose Tissue‐Derived Mesenchymal Stem Cells Have In Vivo Immunosuppressive Properties Applicable for the Control of the Graft‐Versus‐Host Disease , 2006, Stem cells.

[60]  S. Thein,et al.  Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR , 2006, BMC Molecular Biology.

[61]  L. Pénicaud,et al.  Immunomodulatory effect of human adipose tissue‐derived adult stem cells: comparison with bone marrow mesenchymal stem cells , 2005, British journal of haematology.

[62]  Claus Lindbjerg Andersen,et al.  Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets , 2004, Cancer Research.

[63]  M. Pfaffl,et al.  Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations , 2004, Biotechnology Letters.

[64]  D. Bartel.  MicroRNAs Genomics, Biogenesis, Mechanism, and Function , 2004, Cell.

[65]  V. Kim,et al.  The nuclear RNase III Drosha initiates microRNA processing , 2003, Nature.

[66]  F. Speleman,et al.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes , 2002, Genome Biology.

[67]  D. Wright,et al.  Methylphosphate cap structure increases the stability of 7SK, B2 and U6 small RNAs in Xenopus oocytes. , 1993, Nucleic acids research.

[68]  M. Terns,et al.  Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus. , 1993, Genes & development.