In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA.

The skin cells of newborn mice were stably transformed in vivo with the aid of electroporation. The plasmid DNA was introduced subcutaneously followed by high-voltage pulses applied to the skin pleat. NEO-resistant colonies were found in primary cell cultures obtained from the treated skin. The experiments show that in vivo electroporation can be used for the introduction of plasmid DNA into skin cells of mouse.

[1]  G R Stark,et al.  Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. , 1979, Methods in enzymology.

[2]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[3]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[4]  R. Weinberg,et al.  Oncogenes, antioncogenes, and the molecular bases of multistep carcinogenesis. , 1989, Cancer research.

[5]  G. Acsadi,et al.  Direct gene transfer into mouse muscle in vivo. , 1990, Science.

[6]  Michael R. Green,et al.  When the products of oncogenes and anti-oncogenes meet , 1989, Cell.

[7]  H. Ruley.  Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture , 1983, Nature.

[8]  S. Goff,et al.  Germ-line transmission of a c-abl mutation produced by targeted gene disruption in ES cells. , 1989, Science.

[9]  T. Friedmann,et al.  Progress toward human gene therapy. , 1989, Science.

[10]  W. W. Brockman.  Transformation of BALB/c-3T3 cells by tsA mutants of simian virus 40: temperature sensitivity of the transformed phenotype and retransofrmation by wild-type virus , 1978, Journal of virology.

[11]  S. Levenson,et al.  Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes. , 1986, Science.

[12]  P. Kantoff,et al.  In utero gene transfer and expression: a sheep transplantation model. , 1989, Blood.

[13]  K. Iwai,et al.  Increased expression of DNA cointroduced with nuclear protein in adult rat liver. , 1989, Science.

[14]  Y. Barrandon,et al.  Expression of an exogenous growth hormone gene by transplantable human epidermal cells. , 1987, Science.

[15]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[16]  I. Verma,et al.  Expression of a retrovirus encoding human HPRT in mice. , 1984, Science.

[17]  K. Cornetta,et al.  Gene transfer into primates and prospects for gene therapy in humans. , 1989, Progress in nucleic acid research and molecular biology.

[18]  N. Blin,et al.  A general method for isolation of high molecular weight DNA from eukaryotes. , 1976, Nucleic acids research.