Development of Automated Platform for Image Capturing and Counting Algorithm for Viral Plaque

Automatic analyzing tools are essential for biomedical research such as evaluating quantitation of virion. Counting dengue 96-well plaque assay with automated analysis system is one of the interesting and important applications since it can reduce researchers' burden and help researchers analyze the results quickly. Thus, automated platform for image capturing and counting algorithm for viral plaque have been developed. The automated platform for image capturing consists of xy-table, which have work space of 220 mm x 220 mm,accuracy of 0.05 mm, and vertical z-axis for image focusing. With the automated platform, each well of 96-well plate can be automatically captured with high resolution. Next, the counting algorithm is a program for counting and analyzing the images. The algorithm is developed from Halcon library. The results show that the accuracy of the counting algorithm is good enough for the application. Moreover, with this system, there is no additional process of nontransparent liquid to achieve contrast-enhanced image during image capturing. Thus, this system can reduce workload of researchers during assay preparation.

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