Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications.

In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic.

[1]  Federica Chiellini,et al.  Polymeric Materials for Bone and Cartilage Repair , 2010 .

[2]  K. Katti,et al.  Polyelectrolyte-complex nanostructured fibrous scaffolds for tissue engineering , 2009 .

[3]  T. Son,et al.  Polyelectrolyte complex hydrogel composed of chitosan and poly(γ-glutamic acid) for biological application: Preparation, physical properties, and cytocompatibility , 2007 .

[4]  R. B. Sashidhar,et al.  Polyelectrolyte complexes of gum kondagogu and chitosan, as diclofenac carriers , 2009 .

[5]  J. A. Cooper,et al.  Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly(epsilon-caprolactone) fabricated via co-extrusion and gas foaming. , 2008, Acta biomaterialia.

[6]  Hermann Seitz,et al.  Endocultivation: 3D printed customized porous scaffolds for heterotopic bone induction. , 2009, Oral oncology.

[7]  Makarand V Risbud,et al.  Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. , 2005, Biomaterials.

[8]  B. Luppi,et al.  Pectin-based microspheres for colon-specific delivery of vancomycin. , 2009, The Journal of pharmacy and pharmacology.

[9]  D. Maniglio,et al.  Effects on interfacial properties and cell adhesion of surface modification by pectic hairy regions. , 2004, Biomacromolecules.

[10]  Linshu Liu,et al.  An osteoconductive collagen/hyaluronate matrix for bone regeneration. , 1999, Biomaterials.

[11]  Peter Fredericks,et al.  Interactions between alginate and chitosan biopolymers characterized using FTIR and XPS. , 2007, Biomacromolecules.

[12]  R Scapinelli,et al.  Hyaluronan-based biopolymers as delivery vehicles for bone-marrow-derived mesenchymal progenitors. , 2000, Journal of biomedical materials research.

[13]  Joseph Kost,et al.  Pectin-based systems for colon-specific drug delivery via oral route. , 2003, Biomaterials.

[14]  V. Dumas,et al.  The effect of dual frequency cyclic compression on matrix deposition by osteoblast-like cells grown in 3D scaffolds and on modulation of VEGF variant expression. , 2009, Biomaterials.

[15]  Ilídio J Correia,et al.  Development of a new chitosan hydrogel for wound dressing , 2009, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[16]  D. Mohnen Pectin structure and biosynthesis. , 2008, Current opinion in plant biology.

[17]  N. Selvamurugan,et al.  Biocomposites containing natural polymers and hydroxyapatite for bone tissue engineering. , 2010, International journal of biological macromolecules.

[18]  P. Mura,et al.  Development of enteric-coated calcium pectinate microspheres intended for colonic drug delivery. , 2008, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[19]  A. Boccaccini,et al.  Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. , 2006, Biomaterials.

[20]  Molly M. Stevens,et al.  Biomaterials for bone tissue engineering , 2008 .

[21]  S. A. Sande,et al.  Development of pectin matrix tablets for colonic delivery of model drug ropivacaine. , 2000, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[22]  Christos Pappas,et al.  FT-IR spectroscopic determination of the degree of esterification of cell wall pectins from stored peaches and correlation to textural changes , 1998 .

[23]  D. Attwood,et al.  Studies on pectin formulations for colonic drug delivery , 1994 .

[24]  Gabriela A Silva,et al.  Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. , 2007, Advanced drug delivery reviews.

[25]  Severian Dumitriu,et al.  Polysaccharides : structural diversity and functional versatility , 1998 .

[26]  B. Stuart Infrared Spectroscopy , 2004, Analytical Techniques in Forensic Science.

[27]  H. Bessler,et al.  Citrus pectin affects cytokine production by human peripheral blood mononuclear cells. , 2008, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[28]  A. Ramalho,et al.  Films based on chitosan polyelectrolyte complexes for skin drug delivery: Development and characterization , 2008 .

[29]  Robert Gurny,et al.  Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications. , 2004, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[30]  G. Seymour,et al.  Pectins and their manipulation , 2002 .

[31]  R. Reis,et al.  Preparation of chitosan scaffolds loaded with dexamethasone for tissue engineering applications using supercritical fluid technology , 2009 .

[32]  Yubao Li,et al.  Preparation and characterization of a novel composite containing carboxymethyl cellulose used for bone repair , 2009 .

[33]  Yu-Chih Liang,et al.  Suppression of endotoxin-induced proinflammatory responses by citrus pectin through blocking LPS signaling pathways. , 2006, Biochemical pharmacology.

[34]  In-Yong Kim,et al.  Chitosan and its derivatives for tissue engineering applications. , 2008, Biotechnology advances.

[35]  Chaozong Liu,et al.  Design and Development of Three-Dimensional Scaffolds for Tissue Engineering , 2007 .

[36]  R. Muzzarelli Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone , 2009 .

[37]  Feng Gao,et al.  Non-polymerase-cycling-assembly-based chemical gene synthesis: strategies, methods, and progress. , 2008, Biotechnology advances.

[38]  A. Domard,et al.  Physico-chemical studies of the gelation of chitosan in a hydroalcoholic medium. , 2004, Biomaterials.

[39]  M. Morra,et al.  Differentiation of osteoblasts on pectin-coated titanium. , 2008, Biomacromolecules.

[40]  H. S. Azevedo,et al.  Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends , 2007, Journal of The Royal Society Interface.

[41]  Kam W Leong,et al.  Controlled release from fibers of polyelectrolyte complexes. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[42]  G. Dupuis,et al.  Colon-specific drug delivery: Influence of solution reticulation properties upon pectin beads performance. , 2006, International journal of pharmaceutics.

[43]  Xi Mao,et al.  The development and identification of constructing tissue engineered bone by seeding osteoblasts from differentiated rat marrow stromal stem cells onto three-dimensional porous nano-hydroxylapatite bone matrix in vitro. , 2005, Tissue & cell.