Solvent drag on non-electrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone.

Summary. 1   It is shown that the drag force exerted upon water and solutes by water is proportional to the osmotically induced rate of net water transfer across the toad skin. 2   The ratio between the drag effects on two substances is equal to the inverse ratio between their respective free diffusion coefficients. 3   These findings support the assumption that the toad skin contains a porous layer. 4   Neurohypophyseal hormone increases the permeability to both solutes and water, presumably by increasing the pore size in the porous layer. 5   It seems likely that in addition to the porous layer there is a homogeneous layer through which water can pass more readily than can hydrophilic solutes.

[1]  A. Babb,et al.  LIQUID DIFFUSION OF NON-ELECTROLYTES , 1956 .

[2]  E. M. Renkin.  FILTRATION, DIFFUSION, AND MOLECULAR SIEVING THROUGH POROUS CELLULOSE MEMBRANES , 1954, The Journal of general physiology.

[3]  H. Ussing,et al.  The mode of passage of chloride ions through the isolated frog skin. , 1952, Acta physiologica Scandinavica.

[4]  V. Capraro,et al.  Mechanism of Action of Extracts of the Posthypophysis on Water Transport through the Skin of the Frog (Rana esculenta) , 1952, Nature.

[5]  J. Weisiger,et al.  Reagents for the Van Slyke-Folch wet carbon combustion. , 1951, The Journal of biological chemistry.

[6]  D. B. Sheldon,et al.  The measurement of deuterium oxide in body fluids by the falling drop method. , 1951, The Journal of laboratory and clinical medicine.

[7]  F. C. Henriques,et al.  Analytical Procedure for Measurement of Long-Lived Radioactive Sulfur, S35, with Lauritzen Electroscope and Comparison of Electroscope with Special Geiger Counter , 1946 .

[8]  H. Gregor,et al.  Isotopic tracer studies on the movement of water and ions between intestinal lumen and blood. , 1944 .

[9]  J. Folch,et al.  MANOMETRIC CARBON DETERMINATION , 1940 .

[10]  A. Krogh,et al.  The permeability of the skin of frogs to water as determined by D2O and H2O1 , 1935 .

[11]  H. Ussing,et al.  The contributions of diffusion and flow to the passage of D2O through living membranes; effect of neurohypophyseal hormone on isolated anuran skin. , 1953, Acta physiologica Scandinavica.

[12]  I. Edelman,et al.  Self-diffusion and Structure of Liquid Water. III. Measurement of the Self-diffusion of Liquid Water with H2, H3 and O18 as Tracers1 , 1953 .

[13]  H. H. Sisler,et al.  Addition Compounds of Chromium(VI) Oxide with Heterocyclic Nitrogen Bases. II. Reactions with the Lutidines, Vinylpyridines, 2,4,6-Collidine and α-Picoline1 , 1953 .

[14]  H. Ussing.  Some aspects of the application of tracers in permeability studies. , 1952, Advances in enzymology and related subjects of biochemistry.