Prognostic Significance of Two Dimensional AgNOR Evaluation in Local Advanced Rectal Cancer Treated with Chemoradiotherapy.

The prognostic significance of AgNOR proteins in stage II-III rectal cancers treated with chemoradiotherapy was evaluated. Silver staining was applied to the 3μm sections of parafin blocked tissues from 30 rectal cancer patients who received 5-FU based chemoradiotherapy from May 2003 to June 2006. The microscopic displays of the cells were transferred into the computer via a video camera. AgNOR area (nucleolus organizer region area) and nucleus area values were determined as a nucleolus organizer regions area/total nucleus area (NORa/ TNa). The mean NORa/TNa value was found to be 9.02±3.68. The overall survival and disease free survival in the high NORa/TNa (>9.02) patients were 52.2 months and 39.4 months respectively, as compared to 100.7 months and 98.4 months in the low NORa/TNa (<9.02) cases. (p<0.001 and p<0.001 respectively). In addition, the prognosis in the high NORa/TNa patients was worse than low NORa/TNa patients (p<0.05). In terms of overall survival and disease-free survival, a statistically significant negative correlation was found with the value of NORa/TNa in the correlations tests. Cox regression analyses demostrated that overall survival and disease-free survival were associated with lymph node status (negative or positive) and the NORa/TNa value. We suggest that two-dimensional AgNOR evaluation may be a safe and usable parameter for prognosis and an indicator of cell proliferation instead of AgNOR dots.

[1]  Jacob Rosenberg,et al.  Colorectal carcinogenesis--update and perspectives. , 2014, World journal of gastroenterology.

[2]  M. Sideris,et al.  Molecular biomarkers and classification models in the evaluation of the prognosis of colorectal cancer. , 2014, Anticancer research.

[3]  S. Eser,et al.  Cancer incidence rates in Turkey in 2006: a detailed registry based estimation. , 2010, Asian Pacific journal of cancer prevention : APJCP.

[4]  H. Demirtaş,et al.  Two-dimensional agnor evaluation as a prognostic variable in urinary bladder carcinoma: a different approach via total agnor area/nucleus area per cell. , 2007, Micron.

[5]  P. Jeannesson,et al.  Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level , 2005, The Histochemical Journal.

[6]  W. Böcker,et al.  Demonstration of silver-stained nucleolar organizer region associated proteins (AgNORs) after wet autoclave pretreatment in breast carcinoma: Correlation to tumor stage and long-term survival , 2005, Breast Cancer Research and Treatment.

[7]  Joseph Chuen Kwun Lee,et al.  Argyrophilic nuclear organizer regions in nasopharyngeal carcinoma and paraneoplastic epithelia , 2003, Head & neck.

[8]  H. Dönmez,et al.  Condensed chromatin surface and NORs surface enhancement in mitogen-stimulated lymphocytes of Down syndrome patients. , 2001, Annales de genetique.

[9]  M. W. Pak,et al.  Morphometric analysis of argyrophilic nucleolar organizer regions (AgNORs) in nasopharyngeal carcinoma , 2000, Head & neck.

[10]  N. Jonjić,et al.  Prognostic significance of DNA ploidy pattern and nucleolar organizer regions (AgNOR) in colorectal carcinoma. , 2000, Croatian medical journal.

[11]  D. Öfner.  In situ standardised AgNOR analysis: a simplified method for routine use to determine prognosis and chemotherapy efficiency in colorectal adenocarcinoma. , 2000 .

[12]  L. Chiusa,et al.  Prognostic relevance of AgNORs in tumor pathology. , 2000, Micron.

[13]  D. Ofner.  In situ standardised AgNOR analysis: a simplified method for routine use to determine prognosis and chemotherapy efficiency in colorectal adenocarcinoma. , 2000, Micron.

[14]  Y. Tabuchi,et al.  Evaluation of argyrophilic nucleolar organizer region and proliferating cell nuclear antigen in colorectal cancer , 1998, Journal of surgical oncology.

[15]  T. Sugai,et al.  Assessment of the expression of p53, MIB‐1 (Ki‐67 antigen), and argyrophilic nucleolar organizer regions in carcinoma of the extrahepatic bile duct , 1998, Cancer.

[16]  M. Yang,et al.  Usefulness of AgNOR score in differentiating benign from malignant pulmonary aspiration cytology. , 1997, Acta cytologica.

[17]  J. Piffkó,et al.  Standardized demonstration of silver-stained nucleolar organizer regions-associated proteins in archival oral squamous cell carcinomas and adjacent non-neoplastic mucosa. , 1997, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.

[18]  U. Joos,et al.  Prognostic value of histobiological factors (malignancy grading and AgNOR content) assessed at the invasive tumour front of oral squamous cell carcinomas. , 1997, British Journal of Cancer.

[19]  M. Sanz-Anquela,et al.  AgNOR evaluation by image processing methods. Staining modifications and results in 126 invasive ductal breast carcinomas. , 1996, Analytical and quantitative cytology and histology.

[20]  L. Roncucci,et al.  Argyrophilic nucleolar organizer regions and bromodeoxyuridine and 3[H]‐thymidine labelling indices in colorectal cancer , 1995, Cell proliferation.

[21]  T. Inoue,et al.  Nuclear DNA content and nucleolar organizer regions in colorectal cancer , 1995, Journal of surgical oncology.

[22]  Y. Takano,et al.  [Analyses of PCNA and AgNOR on advanced colorectal cancer as predictive indicators of the prognosis]. , 1994, Nihon Geka Gakkai zasshi.

[23]  D. Treré.  Technical and methodological aspects of silver staining and measurement of nucleolar organizer region (NOR). , 1994, Zentralblatt fur Pathologie.

[24]  P. Hufnagl,et al.  Guidelines of AgNOR quantitation , 1994 .

[25]  L. Lindner.  Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). , 1993, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[26]  I. Fonseca,et al.  Adenoid cystic carcinoma: A study of nucleolar organizer regions (AgNOR) counts and their relation to prognosis , 1993, The Journal of pathology.

[27]  T. Tong,et al.  Cancer statistics, 1993 , 1993, CA: a cancer journal for clinicians.

[28]  A. Cancellieri,et al.  AgNOR area in interphase nuclei of human tumours correlates with the proliferative activity evaluated by bromodeoxyuridine labelling and Ki‐67 immunostaining , 1991, The Journal of pathology.

[29]  M. Tötsch,et al.  Silver stained nucleolar organizer region proteins (Ag‐NORs) as a predictor of prognosis in colonic cancer , 1990, The Journal of pathology.

[30]  D. Treré,et al.  Quantity of nucleolar silver-stained proteins is related to proliferating activity in cancer cells. , 1990, Laboratory investigation; a journal of technical methods and pathology.

[31]  A. Cancellieri,et al.  Diagnostic value of silver-stained interphasic nucleolar organizer regions in breast tumors. , 1990, Ultrastructural pathology.

[32]  D. Treré,et al.  The silver-stained proteins of interphasic nucleolar organizer regions as a parameter of cell duplication rate. , 1989, Experimental cell research.

[33]  M. Derenzini,et al.  Distribution of silver-stained interphase nucleolar organizer regions as a parameter to distinguish neoplastic from nonneoplastic reactive cells in human effusions. , 1989, Acta cytologica.

[34]  N. Dubrawsky.  Cancer statistics , 1989, CA: a cancer journal for clinicians.

[35]  M. Derenzini,et al.  Interphasic nucleolar organizer region distribution as a diagnostic parameter to differentiate benign from malignant epithelial tumors of human intestine , 1987, Virchows Archiv. B, Cell pathology including molecular pathology.