Horseshoe Crabs in Modern Day Biotechnological Applications

Horseshoe crabs are an important source of novel bioactive compounds. These species are the source of novel antimicrobial agents, peptides with impending pharmaceutical applications, nutritional supplements, biosensing probes, and other biotechnological implications. Many new bioactive molecules, including an antimicrobial and anticarcinogenic agent with therapeutic applications have been reported from these animals. Even if only a few products are presently on the industrial scale, a number of novel compounds are now under clinical trials and research. Despite the fact that horseshoe crabs offer an enormously lucrative source of novel bioactive compounds, they also face great challenges for their existence. This chapter highlights the advances in the exploitation of horseshoe crabs in modern day biotechnological applications, their survival and conservation.

[1]  IrFC - An Ixodes ricinus injury-responsive molecule related to Limulus Factor C. , 2014, Developmental and comparative immunology.

[2]  S. Iwanaga,et al.  Direct virus inactivation of tachyplesin I and its isopeptides from horseshoe crab hemocytes. , 1991, Chemotherapy.

[3]  T. Katsu,et al.  Mode of action of an antimicrobial peptide, tachyplesin I, on biomembranes. , 1993, Biological & pharmaceutical bulletin.

[4]  H. Aoyagi,et al.  Conformation of tachyplesin I from Tachypleus tridentatus when interacting with lipid matrices. , 1992, Biochemistry.

[5]  T. Obata,et al.  Early detection of the Limulus amebocyte lysate reaction evoked by endotoxins. , 2008, Analytical biochemistry.

[6]  Stephen A. Smith,et al.  The Horseshoe Crab, Limulus polyphemus: 200 Million Years of Existence, 100 Years of Study , 2002 .

[7]  Xiaodong Wang,et al.  Smac, a Mitochondrial Protein that Promotes Cytochrome c–Dependent Caspase Activation by Eliminating IAP Inhibition , 2000, Cell.

[8]  W. Blanckenhorn,et al.  Sex, Size and Gender Roles , 2007 .

[9]  M. Leippe,et al.  Epithelial immunity in a marine invertebrate: a cytolytic activity from a cuticular secretion of the American horseshoe crab, Limulus polyphemus , 2008 .

[10]  T. Miyata,et al.  Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). Isolation and chemical structure. , 1988, The Journal of biological chemistry.

[11]  Jeak Ling Ding,et al.  Endotoxin detection--from limulus amebocyte lysate to recombinant factor C. , 2010, Sub-cellular biochemistry.

[12]  A. Rudloe.  The effect of heavy bleeding on mortality of the horseshoe crab, Limulus polyphemus, in the natural environment , 1983 .

[13]  P. Armstrong,et al.  Initial characterization of a potential anti-fouling system in the American horseshoe crab, Limulus polyphemus. , 2000, The Biological bulletin.

[14]  P. Armstrong.  Role of α2-macroglobulin in the immune responses of invertebrates , 2010 .

[15]  S. Kawabata,et al.  A Newly Identified Horseshoe Crab Lectin with Binding Specificity to O-antigen of Bacterial Lipopolysaccharides* , 1997, The Journal of Biological Chemistry.

[16]  E. Jaenicke,et al.  SDS-induced Phenoloxidase Activity of Hemocyanins fromLimulus polyphemus, Eurypelma californicum, andCancer magister * , 2001, The Journal of Biological Chemistry.

[17]  S. Kawabata,et al.  The complete amino acid sequence of coagulogen isolated from Southeast Asian horseshoe crab, Carcinoscorpius rotundicauda. , 1985, Journal of biochemistry.

[18]  G. Kroemer,et al.  Mitochondria--the Death Signal Integrators , 2000, Science.

[19]  Peng Miao,et al.  Electrochemical sensing strategies for the detection of endotoxin: a review , 2013 .

[20]  J. L. Ding,et al.  Definition of endotoxin binding sites in horseshoe crab Factor C recombinant sushi proteins and neutralization of endotoxin by sushi peptides , 2000, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[21]  S. Iwanaga,et al.  Isolation and biological activities of limulus anticoagulant (anti-LPS factor) which interacts with lipopolysaccharide (LPS). , 1985, Journal of biochemistry.

[22]  M. Hollenberg,et al.  Protease-activated receptors: development of agonists selective for receptors triggered by either thrombin (PAR1) or trypsin (PAR2). , 1997, Proceedings of the Western Pharmacology Society.

[23]  Kefeng Zeng,et al.  A rapid highly-sensitive endotoxin detection system. , 2006, Biosensors & bioelectronics.

[24]  Y. Chen,et al.  RGD-Tachyplesin inhibits tumor growth. , 2001, Cancer research.

[25]  M. L. Botton.  The Ecological Importance of Horseshoe Crabs in Estuarine and Coastal Communities: A Review and Speculative Summary , 2009 .

[26]  M. Koch,et al.  Cyclic antimicrobial peptides based on Limulus anti-lipopolysaccharide factor for neutralization of lipopolysaccharide. , 2004, Biochemical pharmacology.

[27]  J. L. Ding,et al.  Application of cell-free hemolymph of horseshoe crab in antimicrobial drug screening. , 2011, Current pharmaceutical design.

[28]  Steven W. Taylor,et al.  Antimicrobial Peptides from Marine Invertebrates , 2004, Antimicrobial Agents and Chemotherapy.

[29]  Qi-Fu Li,et al.  Effects of tachyplesin on the morphology and ultrastructure of human gastric carcinoma cell line BGC-823. , 2000, World journal of gastroenterology.

[30]  Yong Woo Cho,et al.  Electrochemical endotoxin sensors based on TLR4/MD-2 complexes immobilized on gold electrodes. , 2011, Biosensors & bioelectronics.

[31]  David R. Smith,et al.  Comparative Status and Assessment of Limulus polyphemus with Emphasis on the New England and Delaware Bay Populations , 2009 .

[32]  Qing-Rong Liu,et al.  Effects of tachyplesin on proliferation and differentiation of human hepatocellular carcinoma SMMC-7721 cells. , 2002, World journal of gastroenterology.

[33]  Robert B. Barlow,et al.  The American Horseshoe Crab , 2004 .

[34]  T. Sakmar,et al.  Studies on Limulus amoebocyte lysate. III. Purification of an endotoxin-binding protein from Limulus amoebocyte membranes. , 1980, The Journal of biological chemistry.

[35]  B. Lang,et al.  Mitochondrial evolution. , 1999, Science.

[36]  W. Blanckenhorn,et al.  Sex, size, and gender roles : evolutionary studies of sexual size dimorphism , 2007 .

[37]  T. Liu,et al.  Purification and characterization of an endotoxin-binding protein with protease inhibitory activity from Limulus amebocytes. , 1991, The Journal of biological chemistry.

[38]  S. Cheung,et al.  Hemolymph quality as indicator of health status in juvenile Chinese horseshoe crab Tachypleus tridentatus (Xiphosura) under laboratory culture , 2014 .

[39]  Teodor Gotszalk,et al.  Application of quartz tuning forks for detection of endotoxins and Gram-negative bacterial cells by monitoring of Limulus Amebocyte Lysate coagulation. , 2014, Biosensors & bioelectronics.

[40]  Y. Toh,et al.  Morphology of the granular hemocytes of the Japanese horseshoe crabTachypleus tridentatus and immunocytochemical localization of clotting factors and antimicrobial substances , 1991, Cell and Tissue Research.

[41]  F. Battaglini,et al.  Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin. , 2010, Biosensors & bioelectronics.

[42]  T. Whalley,et al.  Phagocytic activity of Limulus polyphemus amebocytes in vitro. , 2012, Journal of invertebrate pathology.

[43]  Morvan,et al.  In Vitro Activity of the Limulus Antimicrobial Peptide Tachyplesin I on Marine Bivalve Pathogens , 1997, Journal of invertebrate pathology.

[44]  Jiandong Ren,et al.  Lipopolysaccharide (LPS) detoxification of analogue peptides derived from limulus anti-LPS factor , 2010, Peptides.

[45]  H. Aoyagi,et al.  Simplified preparation of crude and functional coagulogen by thermal inactivation of serine proteases in Limulus amebocyte lysate and its application for rapid endotoxin determination. , 2012, Journal of bioscience and bioengineering.

[46]  S. Iwanaga,et al.  Anti‐LPS factor in the horseshoe crab, Tachypleus tridentatus , 1984, FEBS letters.

[47]  S. Iwanaga,et al.  Tachyplesins isolated from hemocytes of Southeast Asian horseshoe crabs (Carcinoscorpius rotundicauda and Tachypleus gigas): identification of a new tachyplesin, tachyplesin III, and a processing intermediate of its precursor. , 1990, Journal of biochemistry.

[48]  Nicholas H. Putnam,et al.  Joint assembly and genetic mapping of the Atlantic horseshoe crab genome reveals ancient whole genome duplication , 2013, GigaScience.

[49]  Kumi Y. Inoue,et al.  A screen-printed endotoxin sensor based on amperometry using a novel p-aminophenol conjugated substrate for a Limulus amebocyte lysate protease reaction. , 2013, The Analyst.

[50]  H. Brockmann,et al.  The evolution and maintenance of sexual size dimorphism in horseshoe crabs: an evaluation of six functional hypotheses , 2014, Animal Behaviour.

[51]  T. Yoneya,et al.  Antimicrobial peptides, isolated from horseshoe crab hemocytes, tachyplesin II, and polyphemusins I and II: chemical structures and biological activity. , 1989, Journal of biochemistry.

[52]  B. Ho,et al.  A new era in pyrogen testing. , 2001, Trends in biotechnology.

[53]  A P Das,et al.  Recent advances in biosensor based endotoxin detection. , 2014, Biosensors & bioelectronics.

[54]  J. Torres,et al.  Structure, activity and interactions of the cysteine deleted analog of tachyplesin-1 with lipopolysaccharide micelle: Mechanistic insights into outer-membrane permeabilization and endotoxin neutralization. , 2012, Biochimica et biophysica acta.

[55]  C. N. Shuster,et al.  A contribution to the population biology of horseshoe crabs,Limulus polyphemus (L.), in Delaware Bay , 1985 .