Pancreatic acinar cells: acetylcholine‐evoked electrical uncoupling and its ionic dependency

1. Isolated segments of mouse or rat pancreas were placed in a Perspex bath through which physiological saline solutions were circulated. Intracellular recordings from surface acini were made by inserting up to three separate micro‐electrodes into neighbouring cells within the same acinus. Through one of these electrodes current pulses could be injected. Electrical coupling between the cells could be continuously assessed. The acinus under investigation was stimulated by iontophoresis of ACh from an extracellular micropipette.

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