Robust Tracing and Visualization of Heterogeneous Microvascular Networks

Advances in high-throughput imaging allow researchers to collect three-dimensional images of whole organ microvascular networks. These extremely large images contain networks that are highly complex, time consuming to segment, and difficult to visualize. In this paper, we present a framework for segmenting and visualizing vascular networks from terabyte-sized three-dimensional images collected using high-throughput microscopy. While these images require terabytes of storage, the volume devoted to the fiber network is <inline-formula><tex-math notation="LaTeX">$\approx 4$</tex-math><alternatives> <inline-graphic xlink:href="govyadinov-ieq1-2818701.gif"/></alternatives></inline-formula> percent of the total volume size. While the networks themselves are sparse, they are tremendously complex, interconnected, and vary widely in diameter. We describe a parallel GPU-based predictor-corrector method for tracing filaments that is robust to noise and sampling errors common in these data sets. We also propose a number of visualization techniques designed to convey the complex statistical descriptions of fibers across large tissue sections—including commonly studied microvascular characteristics, such as orientation and volume.