Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?
暂无分享,去创建一个
[1] Retraction Kiehntopf,et al. Retraction , 1997, Concurr. Comput. Pract. Exp..
[2] S. Bustin,et al. Molecular assessment of tumour stage and disease recurrence using PCR-based assays. , 1998, Molecular medicine today.
[3] S. Bustin. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. , 2000, Journal of molecular endocrinology.
[4] F. Speleman,et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes , 2002, Genome Biology.
[5] Jason E. Stewart,et al. Minimum information about a microarray experiment (MIAME)—toward standards for microarray data , 2001, Nature Genetics.
[6] Liang-sheng Lu,et al. [Expression of fusion proteins in beta(2)GP I gene-transfected HEp-2 cells and its clinical application]. , 2002, Zhonghua yi xue za zhi.
[7] S A Bustin,et al. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. , 2002, Journal of molecular endocrinology.
[8] O. Sheils,et al. Potential viral pathogenic mechanism for new variant inflammatory bowel disease , 2002, Molecular pathology : MP.
[9] K. Buetow,et al. Allelic variation in gene expression is common in the human genome. , 2003, Genome research.
[10] Weiping Ma,et al. Embryogenesis Microarray for Profiling Gene Expression Patterns during 15,000 Unique Zebrafish Est Clusters and Their Future Use in Material Supplemental , 2022 .
[11] Anders Ståhlberg,et al. Comparison of reverse transcriptases in gene expression analysis. , 2004, Clinical chemistry.
[12] M. Kubista,et al. Properties of the reverse transcription reaction in mRNA quantification. , 2004, Clinical chemistry.
[13] Tania Nolan,et al. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. , 2004, Journal of biomolecular techniques : JBT.
[14] Stephen A Bustin,et al. Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. , 2005, Clinical science.
[15] François Parcy,et al. The mRNA of the Arabidopsis Gene FT Moves from Leaf to Shoot Apex and Induces Flowering , 2005, Science.
[16] Nir Hacohen,et al. Minimizing the risk of reporting false positives in large-scale RNAi screens , 2006, Nature Methods.
[17] F. Hufert,et al. Diagnostic Value of Reverse Transcription-PCR for Detection of Cytomegalovirus pp67 in Samples from Solid-Organ Transplant Recipients , 2006, Journal of Clinical Microbiology.
[18] R. Stephens,et al. Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. , 2006, Cancer cell.
[19] S. Bustin. Nucleic acid quantification and disease outcome prediction in colorectal cancer. , 2006, Personalized medicine.
[20] A. O'hare,et al. Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK , 2006, Journal of medical virology.
[21] Stephen A Bustin,et al. Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer. , 2006, Molecular aspects of medicine.
[22] D. Tranchina,et al. Stochastic mRNA Synthesis in Mammalian Cells , 2006, PLoS biology.
[23] Tania Nolan,et al. Quantification of mRNA using real-time RT-PCR , 2006, Nature Protocols.
[24] Angela M Lewis,et al. Prospective multi-institutional study of reverse transcriptase polymerase chain reaction for molecular staging of melanoma. , 2006, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.
[25] Tania Nolan,et al. SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. , 2006, Analytical biochemistry.
[26] C. Sotiriou,et al. Taking gene-expression profiling to the clinic: when will molecular signatures become relevant to patient care? , 2007, Nature Reviews Cancer.
[27] Nigel W. Hardy,et al. The Metabolomics Standards Initiative , 2007, Nature Biotechnology.
[28] A. Bitton,et al. No evidence of persisting measles virus in the intestinal tissues of patients with inflammatory bowel disease , 2007, Gut.
[29] W. Fang,et al. Detection of let-7a MicroRNA by Real-time PCR in Colorectal Cancer: a Single-centre Experience from China , 2007, The Journal of international medical research.
[30] E. Miska,et al. MicroRNA—implications for cancer , 2007, Virchows Archiv.
[31] S. Bustin,et al. Molecular staging of colorectal cancer: new paradigm or waste of time? , 2007, Expert opinion on medical diagnostics.
[32] C. Moreno,et al. Molecular pathogenesis of human prolactinomas identified by gene expression profiling, RT-qPCR, and proteomic analyses , 2008, Pituitary.
[33] A Thank You from Tulane University , 2007, Science.
[34] E. O’Shea,et al. Living with noisy genes: how cells function reliably with inherent variability in gene expression. , 2007, Annual review of biophysics and biomolecular structure.
[35] S. Haney. Increasing the robustness and validity of RNAi screens. , 2007, Pharmacogenomics.
[36] Xiaoyan Lu,et al. Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses , 2007, Journal of Clinical Microbiology.
[37] Nathan M. Springer,et al. Allele-Specific Expression Patterns Reveal Biases and Embryo-Specific Parent-of-Origin Effects in Hybrid Maize[W] , 2007, The Plant Cell Online.
[38] F. Ahmed. Role of miRNA in carcinogenesis and biomarker selection: a methodological view , 2007, Expert review of molecular diagnostics.
[39] Lucila Ohno-Machado,et al. Peripheral arterial occlusive disease: Global gene expression analyses suggest a major role for immune and inflammatory responses , 2008, BMC Genomics.
[40] F. Tinti,et al. BKV QPCR detection and infection monitoring in renal transplant recipients. , 2007, The new microbiologica.
[41] S. M. Power,et al. Clinical significance of the RT‐PCR positive sentinel node in melanoma , 2007, Journal of surgical oncology.
[42] G. Botti,et al. Impact of False-Negative Sentinel Lymph Node Biopsy on Survival in Patients with Cutaneous Melanoma , 2007, Annals of Surgical Oncology.
[43] J. Jassem,et al. BRCA1: A Novel Prognostic Factor in Resected Non-Small-Cell Lung Cancer , 2007, PloS one.
[44] S. Eschrich,et al. The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis , 2008, BMC Medical Genomics.
[45] Jingcao Pan,et al. Preparation of Armored RNA as a Control for Multiplex Real-Time Reverse Transcription-PCR Detection of Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus , 2007, Journal of Clinical Microbiology.
[46] K. Mimori,et al. Clinical Significance of BMP7 in Human Colorectal Cancer , 2008, Annals of Surgical Oncology.
[47] J. Siedlecki,et al. Usefulness of real-time PCR in long-term follow-up of follicular lymphoma patients. , 2007, Acta biochimica Polonica.
[48] C. Ki,et al. Real-time PCR assay compared with antigenemia assay for detecting cytomegalovirus infection in kidney transplant recipients. , 2007, Transplantation proceedings.
[49] Lennart Martens,et al. The minimum information about a proteomics experiment (MIAPE) , 2007, Nature Biotechnology.
[50] J. Peiris,et al. Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions , 2007, Methods in molecular biology.
[51] C. Robson,et al. Application of transcript profiling in formalin‐fixed paraffin‐embedded diagnostic prostate cancer needle biopsies , 2008, BJU international.
[52] E. Labourier,et al. Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues. , 2008, The Journal of molecular diagnostics : JMD.
[53] S. Bustin. Molecular medicine, gene-expression profiling and molecular diagnostics: putting the cart before the horse. , 2008, Biomarkers in medicine.
[54] Heidi J. Peltier,et al. Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues. , 2008, RNA.
[55] Libing Song,et al. Astrocyte Elevated Gene-1 is a Novel Prognostic Marker for Breast Cancer Progression and Overall Patient Survival , 2008, Clinical Cancer Research.
[56] A. Graham,et al. β2microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients , 2008, British Journal of Cancer.
[57] O. Landt,et al. Two-step real-time PCR quantification of all subtypes of human immunodeficiency virus type 1 by an in-house method using locked nucleic acid-based probes. , 2008, Folia medica.
[58] S. Libutti,et al. Identification of Genes Differentially Expressed in Benign versus Malignant Thyroid Tumors , 2008, Clinical Cancer Research.
[59] N. Zakharova,et al. An oligonucleotide microarray for multiplex real-time PCR identification of HIV-1, HBV, and HCV. , 2008, BioTechniques.
[60] S. Ishikawa,et al. Oxysterol binding protein‐related protein‐5 is related to invasion and poor prognosis in pancreatic cancer , 2008, Cancer science.
[61] H. Maeta,et al. p27Kip1 is overexpressed in very early stages of hepatocarcinogenesis , 2008, Cancer science.
[62] Nigel W. Hardy,et al. Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project , 2008, Nature Biotechnology.
[63] R. Solana,et al. Indirect effects of cytomegalovirus infection in solid organ transplant recipients. , 2008, Enfermedades infecciosas y microbiologia clinica.
[64] K. Bendtzen,et al. Blood cell gene expression profiling in subjects with aggressive periodontitis and chronic arthritis. , 2008, Journal of periodontology.
[65] Britta Hartmann,et al. Genome-wide Analysis of Alternative Pre-mRNA Splicing* , 2008, Journal of Biological Chemistry.
[66] D. A. Golden,et al. Transcription analysis of stx1, marA, and eaeA genes in Escherichia coli O157:H7 treated with sodium benzoate. , 2008, Journal of food protection.
[67] RT-qPCR and molecular diagnostics : no evidence for measles virus in the GI tract of autistic children , 2008 .
[68] J. Towbin,et al. Myocardial pro-inflammatory cytokine expression and cellular rejection in pediatric heart transplant recipients. , 2008, The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation.
[69] J. Loeffler,et al. Contribution of new diagnostic approaches to antifungal treatment plans in high-risk haematology patients. , 2008, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.
[70] A. Chinnaiyan,et al. Recurrent gene fusions in prostate cancer , 2008, Nature Reviews Cancer.
[71] K. Bendtzen,et al. Blood cell gene expression profiling in subjects with aggressive periodontitis and chronic arthritis. , 2008, Journal of periodontology.
[72] A. Yeoh,et al. Rapid detection of prognostically important childhood acute lymphoblastic leukemia chimeric transcripts using multiplex SYBR green real-time reverse transcription PCR. , 2008, Annals of clinical and laboratory science.
[73] J. Peiris,et al. Design of Multiplexed Detection Assays for Identification of Avian Influenza A Virus Subtypes Pathogenic to Humans by SmartCycler Real-Time Reverse Transcription-PCR , 2008, Journal of Clinical Microbiology.
[74] Tyler E. Miller,et al. MicroRNA-221/222 Confers Tamoxifen Resistance in Breast Cancer by Targeting p27Kip1*♦ , 2008, Journal of Biological Chemistry.
[75] H. Hirsch,et al. Cidofovir Inhibits Polyomavirus BK Replication in Human Renal Tubular Cells Downstream of Viral Early Gene Expression , 2008, American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons.
[76] D. Berger,et al. MicroRNA and Colorectal Cancer , 2009, World Journal of Surgery.
[77] U. Kammula,et al. Use of high throughput qPCR screening to rapidly clone low frequency tumour specific T-cells from peripheral blood for adoptive immunotherapy , 2008, Journal of Translational Medicine.
[78] Y. Nagano,et al. Clinicopathological significance of the gene expression of matrix metalloproteinases and reversion-inducing cysteine-rich protein with Kazal motifs in patients with colorectal cancer: MMP-2 gene expression is a useful predictor of liver metastasis from colorectal cancer. , 2008, Oncology reports.
[79] Martin Hemberg,et al. Quantification of mRNA in single cells and modelling of RT-qPCR induced noise , 2008, BMC Molecular Biology.
[80] T. Moritz,et al. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. , 2008, Plant biotechnology journal.
[81] Jianjun Yu,et al. A first-generation multiplex biomarker analysis of urine for the early detection of prostate cancer. , 2008, Cancer research.
[82] R. Britto,et al. Novel Glioblastoma Markers with Diagnostic and Prognostic Value Identified through Transcriptome Analysis , 2008, Clinical Cancer Research.
[83] M. Pirmohamed,et al. Expression of the Uptake Drug Transporter hOCT1 is an Important Clinical Determinant of the Response to Imatinib in Chronic Myeloid Leukemia , 2008, Clinical pharmacology and therapeutics.
[84] Chris F. Taylor,et al. The minimum information about a genome sequence (MIGS) specification , 2008, Nature Biotechnology.
[85] Qiong Shao,et al. MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis. , 2008, RNA.
[86] R. Cavallo,et al. Quantitative Detection of Epstein-Barr Virus in Bronchoalveolar Lavage From Transplant and Nontransplant Patients , 2008, Transplantation.
[87] K. Mimori,et al. PDGF-BB is a Novel Prognostic Factor in Colorectal Cancer , 2008, Annals of Surgical Oncology.
[88] A. Falsey,et al. Detection of genetic lineages of human metapneumovirus in Croatia during the winter season 2005/2006 , 2008, Journal of medical virology.
[89] Jack M. Gallup,et al. Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions , 2006, Biological Procedures Online.
[90] D. Kaye,et al. Increased vascular endothelial growth factor mRNA in endomyocardial biopsies from allografts demonstrating severe acute rejection: a longitudinal study. , 2008, Transplant immunology.
[91] W. Bellini,et al. Lack of Association between Measles Virus Vaccine and Autism with Enteropathy: A Case-Control Study , 2008, PloS one.
[92] M. Dugas,et al. Identification of acute myeloid leukaemia associated microRNA expression patterns , 2007, British journal of haematology.
[93] George A Calin,et al. MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia. , 2008, Blood.
[94] D. Mock,et al. Profiling motility signal-specific genes in primary human keratinocytes. , 2008, The Journal of investigative dermatology.
[95] S. Ferretti,et al. A Novel Gene Signature for Molecular Diagnosis of Human Prostate Cancer by RT-qPCR , 2008, PloS one.
[96] S. Koscielny. Critical review of microarray-based prognostic tests and trials in breast cancer , 2008, Current opinion in obstetrics & gynecology.
[97] C. Fiori,et al. Highly specific detection of prostate‐specific antigen‐positive cells in the blood of patients with prostate cancer or benign prostatic hyperplasia, using a real‐time reverse‐transcription‐polymerase chain reaction method with improved sensitivity , 2008, BJU international.
[98] C. Croce,et al. MicroRNA profiling as a tool to understand prognosis, therapy response and resistance in breast cancer. , 2008, European journal of cancer.
[99] Torsten Haferlach,et al. Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin , 2008, Proceedings of the National Academy of Sciences.
[100] Frank J. Sørensen,et al. Diagnostic and prognostic microRNAs in stage II colon cancer. , 2008, Cancer research.
[101] Xiaowei Chen,et al. Allelic imbalance in BRCA1 and BRCA2 gene expression is associated with an increased breast cancer risk. , 2008, Human molecular genetics.
[102] K. Coombes,et al. HOX expression patterns identify a common signature for favorable AML , 2008, Leukemia.
[103] T. Gotoda,et al. Identification of prognostic biomarkers in gastric cancer using endoscopic biopsy samples , 2008, Cancer science.
[104] H. Baba,et al. Detection of colorectal cancer cells from feces using quantitative real‐time RT‐PCR for colorectal cancer diagnosis , 2008, Cancer science.
[105] Melissa A. Brown,et al. Pre-mRNA splicing aberrations and cancer. , 2008, Frontiers in bioscience : a journal and virtual library.
[106] G. Jahn,et al. Rapid Semiquantitative Real-Time PCR for the Detection of Human Cytomegalovirus UL97 Mutations Conferring Ganciclovir Resistance , 2008, Antiviral therapy.
[107] M. Hertz,et al. Bronchoalveolar Lavage Cell Gene Expression in Acute Lung Rejection: Development of a Diagnostic Classifier , 2008, Transplantation.
[108] R. Kodet,et al. Quantitative Measurement of Cyclin D1 mRNA, a Potent Diagnostic Tool to Separate Mantle Cell Lymphoma From Other B-cell Lymphoproliferative Disorders , 2008, Diagnostic molecular pathology : the American journal of surgical pathology, part B.
[109] Kenichi Sugihara,et al. Clinical Significance of High Mobility Group A2 in Human Gastric Cancer and Its Relationship to let-7 MicroRNA Family , 2008, Clinical Cancer Research.
[110] A. Skotnicki,et al. Detection of specific lytic and latent transcripts can help to predict the status of Epstein-Barr virus infection in transplant recipients with high virus load. , 2008, Acta biochimica Polonica.
[111] B. Ponder,et al. Allele-Specific Up-Regulation of FGFR2 Increases Susceptibility to Breast Cancer , 2008, PLoS biology.
[112] M. Fiorentino,et al. Real Time RT-PCR Approach for the Evaluation of ERBB2 Overexpression in Breast Cancer Archival Samples: A Comparative Study With FISH, SISH, and Immunohistochemistry , 2008, Diagnostic molecular pathology : the American journal of surgical pathology, part B.
[113] Thomas D. Schmittgen,et al. Real-time PCR quantification of precursor and mature microRNA. , 2008, Methods.
[114] S. Vacher,et al. Large-scale real-time reverse transcription-PCR approach of angiogenic pathways in human transitional cell carcinoma of the bladder: identification of VEGFA as a major independent prognostic marker. , 2009, European urology.
[115] Jie Cao,et al. Upregulation of Activator Protein-4 in Human Colorectal Cancer With Metastasis , 2009, International journal of surgical pathology.
[116] J. Lang. False Negative Sentinel Lymph Node Biopsies in Melanoma May Result From Deficiencies in Nuclear Medicine, Surgery, or Pathology , 2009 .
[117] J. Kawai,et al. Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach , 2009, PloS one.
[118] V. Beneš,et al. Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis. , 2009, AIDS research and human retroviruses.
[119] 本山 一夫. Clinical significance of high mobility group A2 in human gastric cancer and its relationship to let-7 microRNA family , 2009 .
[120] V. Beneš,et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. , 2009, Clinical chemistry.