Design and construction of a microscope‐based Raman system

A relatively inexpensive micro‐Raman system featuring confocal imaging, holographic optics, and CCD detection is described in detail. The construction of a confocal filter is discussed, along with the depth of field and spatial rejection expected. Limitations of the system such as low‐frequency signal rejection by the holographic optics and wavelength calibration are also discussed. Spectra are shown giving examples of the capabilities of the system and the signal‐to‐noise ratio expected for typical liquid and solid samples.

[1]  M. Delhaye,et al.  Raman microprobe and microscope with laser excitation , 1975 .

[2]  J. Greve,et al.  Chevron-Type Dielectric Filter Set for Efficient Narrow-Band Laser Line Rejection in Raman Microspectrometers , 1994 .

[3]  A. L. Verma,et al.  Characterization of Materials by Micro-Raman Spectroscopy , 1990 .

[4]  Tony Wilson,et al.  Three‐dimensional imaging in confocal imaging systems with finite sized detectors , 1988 .

[5]  G. J. Brakenhoff,et al.  3‐D image formation in high‐aperture fluorescence confocal microscopy: a numerical analysis , 1990 .

[6]  D. Chase,et al.  FT-Raman Microscopy: Discussion and Preliminary Results , 1989 .

[7]  Gerwin J. Puppels,et al.  Description and performance of a highly sensitive confocal Raman microspectrometer , 1991 .

[8]  Michael D. Morris,et al.  Principles and spectroscopic applications of volume holographic optics , 1993 .

[9]  Edgar S. Etz,et al.  The Analysis of Discrete Fine Particles by Raman Spectroscopy , 1975 .

[10]  K. Nakamoto,et al.  Laboratory Raman spectroscopy , 1984 .

[11]  J. Thomas Brownrigg,et al.  Wavelength Calibration Methods for Low-Resolution Photodiode Array Spectrometers , 1993 .

[12]  Derek J. Gardiner,et al.  Line‐scanned micro Raman spectroscopy using a cooled CCD imaging detector , 1990 .

[13]  S. Asher,et al.  Development of a New Optical Wavelength Rejection Filter: Demonstration of its Utility in Raman Spectroscopy , 1984 .

[14]  W. H. Nelson,et al.  Ultraviolet micro-Raman spectrograph for the detection of small numbers of bacterial cells , 1993 .

[15]  M. Bonner Denton,et al.  Applications of Charge Transfer Devices in Spectroscopy , 1988 .

[16]  G. J. Brakenhoff,et al.  Confocal scanning light microscopy with high aperture immersion lenses , 1979 .