Expression of Cytokine mRNA in Leukemic Cells from Adult T Cell Leukemia Patients

HTLV‐I infection of peripheral mature T cells appears to induce the expression of cellular genes including those of some cytokines and their receptors. We examined the expression of interleukin‐lα (IL‐lα), IL‐lβ, IL‐2, IL‐3, IL‐4 and granulocyte/macrophage colony‐stimulating factor (GM‐CSF) at the mRNA level in fresh leukemic cells from 20 adult T cell leukemia patients to see whether there is any association between cytokine expression and MTLV‐I expression and between their expression and clinical manifestations such as hypercalcemia or neutrophilia. IL‐lα, IL‐Iβ and IL‐3 expression was observed in 3, 7 and 1 of 20 cases examined, respectively. However, there seemed to be no association between IL‐1 expression and clinical manifestations. IL‐2, IL‐4 and GM‐CSF mRNA expression was not detected. HTLV‐I viral RNA expression was detected only in one case in which IL‐3 mRNA was expressed in both peripheral blood and lymph node cells and a relatively high proportion of leukemic cells expressed IL‐2 receptor (p55, Tac). Thus, in the present study we could not find any correlation between cytokine expression and HTLV‐I expression in peripheral blood fresh leukemic cells except in one unusual case.

[1]  T. Uchiyama,et al.  Leukemic cells from a chronic T-lymphocytic leukemia patient proliferated in response to both interleukin-2 and interleukin-4 without prior stimulation and produced interleukin-2 mRNA with stimulation. , 1988, Blood.

[2]  T. Uchiyama,et al.  Leukemic cells from some adult T-cell leukemia patients proliferate in response to interleukin-4. , 1988, Blood.

[3]  K. Pfizenmaier,et al.  IL-2 production in human T lymphotropic virus I-infected leukemic T lymphocytes analyzed by in situ hybridization. , 1988, Journal of immunology.

[4]  K. Arai,et al.  Coordinate regulation of immune and inflammatory responses by T cell‐derived lymphokines , 1988, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[5]  W. Greene,et al.  Interleukin 1 gene expression in adult T cell leukemia. , 1987, The Journal of clinical investigation.

[6]  M. Siekevitz,et al.  Activation of interleukin 2 and interleukin 2 receptor (Tac) promoter expression by the trans-activator (tat) gene product of human T-cell leukemia virus, type I. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Y. Yamamoto,et al.  Heterogeneity in response to interleukin 2 and interleukin 2-producing ability of adult T cell leukemic cells. , 1987, Journal of immunology.

[8]  T. Stueve,et al.  Interleukin 1 stimulates endothelial cells to release multilineage human colony-stimulating activity. , 1987, Journal of immunology.

[9]  T. Taniguchi,et al.  Induction of interleukin 2 receptor gene expression by p40x encoded by human T‐cell leukemia virus type 1. , 1986, The EMBO journal.

[10]  Yu-Chung Yang,et al.  Human IL-3 (multi-CSF): Identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3 , 1986, Cell.

[11]  Y. Yamamoto,et al.  Autocrine growth of interleukin 2-producing leukemic cells in a patient with adult T cell leukemia. , 1986, Blood.

[12]  T. Honjo,et al.  Interleukin 1α mRNA in virus-transformed T and B cells , 1986 .

[13]  K. Arai,et al.  Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[14]  T. Honjo,et al.  Origin of human T-lymphotrophic virus I-positive T cell lines in adult T cell leukemia. Analysis of T cell receptor gene rearrangement , 1985, The Journal of experimental medicine.

[15]  J. Yodoi,et al.  Interleukin-2 receptor (Tac antigen) expressed on adult T cell leukemia cells. , 1985, The Journal of clinical investigation.

[16]  T. Taniguchi,et al.  Expression of the pX gene of HTLV-I: general splicing mechanism in the HTLV family. , 1985, Science.

[17]  C. March,et al.  Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs , 1985, Nature.

[18]  T. Waldmann,et al.  Augmented T cell growth factor receptor expression in HTLV-1-infected human leukemic T cells. , 1984, Journal of immunology.

[19]  M. Reitz,et al.  Methylation of human T-cell leukemia virus proviral DNA and viral RNA expression in short- and long-term cultures of infected cells. , 1984, Virology.

[20]  K. Yamaguchi,et al.  Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Yoshida,et al.  Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[22]  T. Taniguchi,et al.  Structure and expression of a cloned cDNA for human interleukin-2 , 1983, Nature.

[23]  A. Gazdar,et al.  Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor , 1981, The Journal of experimental medicine.

[24]  K. Nagata,et al.  Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[25]  K. Takatsuki,et al.  Surface phenotype of Japanese adult T-cell leukemia cells characterized by monoclonal antibodies , 1981 .

[26]  T. Waldmann,et al.  A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. , 1981, Journal of immunology.

[27]  S. Collins,et al.  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture , 1977, Nature.

[28]  H. Boedtker,et al.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. , 1977, Biochemistry.

[29]  J. Yodoi,et al.  Adult T-cell leukemia: clinical and hematologic features of 16 cases. , 1977, Blood.

[30]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[31]  T. Uchiyama,et al.  Close association between interleukin 2 receptor mRNA expression and human T cell leukemia/lymphoma virus type I viral RNA expression in short-term cultured leukemic cells from adult T cell leukemia patients. , 1988, The Journal of clinical investigation.