Prevention of Dielectric Breakdown of Nanopore Membranes by Charge Neutralization

To achieve DNA sequencing using a solid-state nanopore, it is necessary to reduce the electric noise current. The noise current can be decreased by reducing the capacitance (C) of the nanopore device. However, we found that an electric-charge difference (ΔQ) between the electrolyte in one chamber and the electrolyte in another chamber occurred. For low capacitance devices, this electric-charge imbalance can lead to unexpectedly high voltage (ΔV = ΔQ/C) which disrupted the membrane when the two electrolytes were independently poured into the chambers. We elucidated the mechanism for the generation of initial defects and established new procedures for preventing the generation of defects by connecting an electric bypass between the chambers.

[1]  M. Niederweis,et al.  Nanopore DNA sequencing with MspA , 2010, Proceedings of the National Academy of Sciences.

[2]  Kyle Briggs,et al.  Nanopore Fabrication by Controlled Dielectric Breakdown , 2014, PloS one.

[3]  A. Reina,et al.  Graphene as a sub-nanometer trans-electrode membrane , 2010, Nature.

[4]  N. Aluru,et al.  DNA base detection using a single-layer MoS2. , 2014, ACS nano.

[5]  Qiang Xu,et al.  Tailoring the hydrophobicity of graphene for its use as nanopores for DNA translocation , 2013, Nature Communications.

[6]  Kyeong-Beom Park,et al.  A Low-Noise Solid-State Nanopore Platform Based on a Highly Insulating Substrate , 2014, Scientific Reports.

[7]  N H Dekker,et al.  Noise in solid-state nanopores , 2008, Proceedings of the National Academy of Sciences.

[8]  M. Wanunu,et al.  Programmed synthesis of freestanding graphene nanomembrane arrays. , 2015, Small.

[9]  Sanmeet S. Chahal,et al.  Kinetics of nanopore fabrication during controlled breakdown of dielectric membranes in solution , 2015, Nanotechnology.

[10]  Ki-Bum Kim,et al.  Noise and its reduction in graphene based nanopore devices , 2013, Nanotechnology.

[11]  A. Balan,et al.  Differentiation of short, single-stranded DNA homopolymers in solid-state nanopores. , 2013, ACS nano.

[12]  M. Taniguchi,et al.  Single-molecule sensing electrode embedded in-plane nanopore , 2011, Scientific reports.

[13]  M. Mayer,et al.  Noise and bandwidth of current recordings from submicrometer pores and nanopores. , 2008, ACS nano.

[14]  D. Branton,et al.  Characterization of individual polynucleotide molecules using a membrane channel. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[15]  D. Branton,et al.  The potential and challenges of nanopore sequencing , 2008, Nature Biotechnology.

[16]  Jingmin Jin,et al.  Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors. , 2010, Nature nanotechnology.

[17]  J. Hall.  Access resistance of a small circular pore , 1975, The Journal of general physiology.

[18]  Neil Peterman,et al.  DNA translocation through graphene nanopores. , 2010, Nano letters.

[19]  C. Dekker.  Solid-state nanopores. , 2007, Nature nanotechnology.

[20]  Rena Akahori,et al.  Fabricating nanopores with diameters of sub-1 nm to 3 nm using multilevel pulse-voltage injection , 2014, Scientific Reports.

[21]  J. Oosterlaan,et al.  Executive Functioning in Highly Talented Soccer Players , 2014, PloS one.

[22]  Tomio Iwasaki,et al.  Slowing single-stranded DNA translocation through a solid-state nanopore by decreasing the nanopore diameter , 2014, Nanotechnology.

[23]  K. Shepard,et al.  Integrated nanopore sensing platform with sub-microsecond temporal resolution , 2012, Nature Methods.

[24]  Kenneth L. Shepard,et al.  Improving signal-to-noise performance for DNA translocation in solid-state nanopores at MHz bandwidths. , 2014, Nano letters.

[25]  Ke Liu,et al.  Atomically thin molybdenum disulfide nanopores with high sensitivity for DNA translocation. , 2014, ACS nano.

[26]  T. Sorsch,et al.  Nanopores in solid-state membranes engineered for single molecule detection , 2010, Nanotechnology.

[27]  X. Bai,et al.  DNA Translocation through Hydrophilic Nanopore in Hexagonal Boron Nitride , 2013, Scientific Reports.

[28]  T. Saiki,et al.  Optical detection of DNA translocation through silicon nanopore by ultraviolet light , 2014 .

[29]  Andre Marziali,et al.  Noise analysis and reduction in solid-state nanopores , 2007 .

[30]  Cees Dekker,et al.  Modeling the conductance and DNA blockade of solid-state nanopores , 2011, Nanotechnology.