Image analysis of cell natural fluorescence: diagnostic applications in haematology

Haematic cells, excited with radiation of suitable wavelength, give rise to a natural fluorescence (NF) emission. This paper investigates NF to develop new techniques for applications in both basic research and medical diagnostics. Results show that the cell populations examined exhibit peculiar emission bands. The intracellular fluorescence pattern reveals that flouresence is mainly located at cytoplasmic-level, thus related to the metabolic processes of the cells. The photophysical properties of cells appear different among the normal populations and between normal and leukaemic ones. Therefore the recognition of the various cellular elements, according to their fluorescence emission, is possible.

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