Total phenol, catechin, and caffeine contents of teas commonly consumed in the United kingdom.

Levels of total phenol, catechins, and caffeine in teas commonly consumed in the United Kingdom have been determined using reversed phase high-performance liquid chromatography. Tea bags or tea leaves were purchased from local supermarkets and extracted in boiling water for 5 min. The resulting data showed considerable variability in both total phenols [80.5-134.9 mg/g of dry matter (DM) in black teas and 87-106.2 mg/g of DM in green teas] and catechins (5.6-47.5, 51.5-84.3, and 8.5-13.9 mg/g of DM in black, green, and fruit teas, respectively); this was most probably a result of differing agronomic conditions, leaf age, and storage during and after transport, as well as the degree of fermentation. Caffeine contents of black teas (22-28 mg/g of DM) were significantly higher than in less fermented green teas (11-20 mg/g of DM). The relative concentration of the five major tea catechins ranked EGCG > ECG > EC > EGC > C. The estimated U.K. dietary intakes of total tea catechins, calculated on the basis of an average tea consumption of three cups of tea (200 mL cup, 1% tea leaves w/v), were 61.5, 92.7, and 405.5 mg/day from fruit teas, black teas, and green teas, respectively. The coefficients of variation were 19.4, 88.6, and 17.3%, respectively, indicating the wide variation in these intakes. The calculated caffeine intake ranged between 92 and 146 mg/day. In addition, many individuals will consume much larger quantities of tea, of various strengths (as determined by the brewing conditions employed). This broad spread of U.K. daily intakes further emphasizes the need for additional research to relate intake and effect in various population groups.

[1]  Ann C. Noble,et al.  Sensory evaluation of bitterness and astringency of 3R(−)‐epicatechin and 3S(+)‐catechin , 1995 .

[2]  Huafu Wang,et al.  Isocratic elution system for the determination of catechins, caffeine and gallic acid in green tea using HPLC , 2000 .

[3]  D Kromhout,et al.  Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands. , 1993, Nutrition and cancer.

[4]  S. Wiseman,et al.  Antioxidants in tea. , 1997, Critical reviews in food science and nutrition.

[5]  U. Engelhardt,et al.  Flavonoids and other polyphenols in consumer brews of tea and other caffeinated beverages. , 2000, Journal of agricultural and food chemistry.

[6]  P. Fernández,et al.  HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas. , 2000, The Analyst.

[7]  W. Jongen,et al.  A RP-HPLC method for the determination of tea catechins. , 1997, Cancer letters.

[8]  A. Törrönen,et al.  Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. , 1999, Journal of agricultural and food chemistry.

[9]  Determination of tea catechins by reserved phase high performance liquid chromotography , 1998 .

[10]  H N Graham,et al.  Green tea composition, consumption, and polyphenol chemistry. , 1992, Preventive medicine.

[11]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .

[12]  Jen-kun Lin,et al.  Survey of Catechins, Gallic Acid, and Methylxanthines in Green, Oolong, Pu-erh, and Black Teas , 1998 .