Gold nanocages with built-in artificial antibodies for label-free plasmonic biosensing.

We demonstrate that gold nanocages (AuNCs) with built-in artificial antibodies enable the detection of kidney injury biomarker from synthetic urine down to a concentration of 25 ng ml-1. Molecularly imprinted AuNCs exhibit excellent selectivity against numerous interfering urinary proteins and remarkable stability over a wide range of pH and specific gravity.

[1]  T. Nickolas,et al.  Urinary neutrophil gelatinase-associated lipocalin distinguishes pre-renal from intrinsic renal failure and predicts outcomes. , 2011, Kidney international.

[2]  P. Nordlander,et al.  Plasmons in strongly coupled metallic nanostructures. , 2011, Chemical reviews.

[3]  Jianfang Wang,et al.  Shape- and size-dependent refractive index sensitivity of gold nanoparticles. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[4]  Xinmai Yang,et al.  Photoacoustic tomography of a rat cerebral cortex in vivo with au nanocages as an optical contrast agent. , 2007, Nano letters.

[5]  J. Hafner,et al.  A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods. , 2008, ACS nano.

[6]  R. V. Van Duyne,et al.  A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles. , 2002, Journal of the American Chemical Society.

[7]  Kevin Barraclough,et al.  I and i , 2001, BMJ : British Medical Journal.

[8]  Younan Xia,et al.  Facile synthesis of Ag nanocubes and Au nanocages , 2007, Nature Protocols.

[9]  Fujio Mizukami,et al.  A method for the molecular imprinting of hemoglobin on silica surfaces using silanes. , 2005, Biomaterials.

[10]  Jun Wan,et al.  A bioresponsive controlled-release biosensor using Au nanocages capped with an aptamer-based molecular gate and its application in living cells. , 2012, Chemical communications.

[11]  Younan Xia,et al.  Near-infrared gold nanocages as a new class of tracers for photoacoustic sentinel lymph node mapping on a rat model. , 2009, Nano letters.

[12]  Adam D. McFarland,et al.  A Nanoscale Optical Biosensor: Real-Time Immunoassay in Physiological Buffer Enabled by Improved Nanoparticle Adhesion , 2003 .

[13]  A. Garg,et al.  Biomarkers predict progression of acute kidney injury after cardiac surgery. , 2012, Journal of the American Society of Nephrology : JASN.

[14]  Younan Xia,et al.  Increased sensitivity of surface plasmon resonance of gold nanoshells compared to that of gold solid colloids in response to environmental changes. , 2002, Analytical chemistry.

[15]  J. Hafner,et al.  Localized surface plasmon resonance sensors. , 2011, Chemical reviews.

[16]  Jaeyoung Lee,et al.  Nanoparticle-enhanced surface plasmon resonance detection of proteins at attomolar concentrations: comparing different nanoparticle shapes and sizes. , 2012, Analytical chemistry.

[17]  S. Maier,et al.  Plasmonics: Localization and guiding of electromagnetic energy in metal/dielectric structures , 2005 .

[18]  Younan Xia,et al.  Gold nanocages: from synthesis to theranostic applications. , 2011, Accounts of chemical research.

[19]  Mikael Käll,et al.  Refractometric sensing using propagating versus localized surface plasmons: a direct comparison. , 2009, Nano letters.

[20]  Feng Gao,et al.  In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages. , 2010, ACS nano.

[21]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.

[22]  M. El-Sayed,et al.  Gold nanoframes: very high surface plasmon fields and excellent near-infrared sensors. , 2010, Journal of the American Chemical Society.

[23]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[24]  Limei Tian,et al.  Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing , 2013, Advanced functional materials.

[25]  R. V. Van Duyne,et al.  Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor. , 2005, Journal of the American Chemical Society.

[26]  L. Lechuga,et al.  LSPR-based nanobiosensors , 2009 .

[27]  Federico Capasso,et al.  Fano resonances in plasmonic nanoclusters: geometrical and chemical tunability. , 2010, Nano letters.

[28]  R. V. Van Duyne,et al.  A comparative analysis of localized and propagating surface plasmon resonance sensors: the binding of concanavalin a to a monosaccharide functionalized self-assembled monolayer. , 2004, Journal of the American Chemical Society.

[29]  Shoufang Xu,et al.  Recent advances in molecular imprinting technology: current status, challenges and highlighted applications. , 2011, Chemical Society reviews.

[30]  Jeffrey N. Anker,et al.  Biosensing with plasmonic nanosensors. , 2008, Nature materials.