Correlation analysis of the characteristics of ALIP with AML relapse

Objective:To investigate the relevance of abnormal localization of immature precursors(ALIP) aggregates and ALIP clusters with the relapse of acute myelocytic leukemia(AML).Method:Samples of bone marrow from AML patients were collected after chemotherapy.The cases with less than 5% blasts in BM smears and without ALIP in bone marrow biopsy are defined as real CR,the cases with more than 5% bone marrow blasts are defined as typical relapse.ALIP aggregates are regarded as more than five immature precursors away from trabeculae in the marrow,while three to five immature precursors is ALIP clusters.We observed ALIP aggregates and ALIP clusters in bone marrow biopsy among 31 AML patients after real CR until relapse.Result:ALIP is observed in 22 patients among 31 AML patients.the incidence is 71%.The occurrence of ALIP is obviously correlated with relapse of AML(P0.05),moreover,the correlation of ALIP aggregates with relapse is more significant than ALIP clusters(P0.05).The cases with ALIP aggregates usually relapse in(77±35) days,while ALIP clusters is(150±43) days.Conclusion:ALIP in bone marrow biopsy is earlier than bone marrow smears in detection of relapse,especially ALIP aggregates.The AML patients with ALIP usually relapse in a short period of time.