论文信息 - Raman microspectroscopy of LiDNA fibres in ethanol and tert-butanol

Raman microspectroscopy of LiDNA fibres in ethanol and tert-butanol

The conformation of low-salt LiDNA in fibrous form has been studied by Raman microspectroscopy. The recorded spectrum of a fibre soaked in ethanol is very similar to that of a fibre at low relative humidity. From x-ray experiments it is known that in the latter case the DNA adopts the C conformation. Therefore, it can be concluded that this is also the case in a low-salt LiDNA fibre soaked in ethanol. Further, it has been observed that the DNA conformation did not change when the excess ethanol was allowed to evaporate. The C form is also found when the fibre is in contact with ethanol vapour only. These results are in agreement with x-ray studies. However, our experiments with a LiDNA fibre in tert-butanol are not in agreement with x-ray results. Only when the tert-butanol is absolutely free from water is a state intermediate between the B and C forms found.

J. Greve | F. F. Mul | C. Otto | J. Mud

[1] R. Wartell,et al. Changes in Raman vibrational bands of calf thymus DNA during the B‐to‐A transition , 1982, Biopolymers.

[2] H R Drew,et al. Structure of a B-DNA dodecamer. III. Geometry of hydration. , 1981, Journal of molecular biology.

[3] B. H. Pheiffer,et al. Does DNA adopt the C form in concentrated salt solutions or in organic solvent water mixtures? An x-ray diffraction study of DNA fibers immersed in various media. , 1980, Journal of molecular biology.

[4] B. H. Pheiffer,et al. A direct demonstration that the ethanol-induced transition of DNA is between the A and B forms: an X-ray diffraction study. , 1979, Journal of molecular biology.

[5] D. C. Goodwin,et al. Form of DNA and the nature of interactions with proteins in chromatin. , 1978, Nucleic acids research.

[6] W. Peticolas,et al. Determination of the backbone structure of nucleic acids and nucleic acid oligomers by laser Raman scattering. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[7] D. Marvin,et al. The molecular configuration of deoxyribonucleic acid. III. X-ray diffraction study of the C form of the lithium salt. , 1961, Journal of molecular biology.