The Effect of Propofol Anaesthesia on Haematological and Biochemical Blood Profile of European Whitefish

Propofol (2’6-diizopropylofenol) effectively induced general anaesthesia in whitefish in the preliminary test. The aim of the study was to assess the effects of propofol on European whitefish by hematological and biochemical indices of blood plasma. A total of 60 specimens of whitefish from experimental hatchery of Inland Fisheries Institute in Olsztyn were used. Fish were exposed to the water solution of 0.5 cm 3 dm -3 of Diprivan (Astra Zeneca Ltd., UK) (5 mg dm -3 of propofol) for 10 min. Hematological and biochemical indices were analyzed immediately after anaesthesia and 24h after exposure to the anaesthetic, and compared to respective control groups. Propofol did not affect significantly red blood cells indices. Reactive granulocytosis was observed 24 h after anaesthesia. The level of stress indices like: white blood cell count, glucose, albumin and globulin concentration was recovered 24 h after propofol anaesthesia. However, elevated value of alkaline phosphatase suggested some liver disturbance.

[1]  K. Kupren,et al.  Acute ammonia toxicity during early ontogeny of chub, Leuciscus cephalus (Cyprinidae) , 2011 .

[2]  M. Szczepkowski,et al.  Impact of daily feed rations for juvenile common whitefish Coregonus lavaretus (L.), on rearing indicators and oxygen requirements , 2011 .

[3]  S. Siikavuopio,et al.  Growth and mortality of Arctic charr and European whitefish reared at low temperatures , 2010, Hydrobiologia.

[4]  M. Szczepkowski,et al.  Growth rate and reproduction of a brood stock of European whitefish (Coregonus lavaretus L.) from Lake Gaładuś under controlled rearing conditions , 2010 .

[5]  M. Woźny,et al.  Microcystin-LR induced apoptosis and mRNA expression of p53 and cdkn1a in liver of whitefish (Coregonus lavaretus L.). , 2009, Toxicon : official journal of the International Society on Toxinology.

[6]  Tvsp Murthy.  Propofol in Neurotrauma , 2008, Indian Journal of Neurotrauma.

[7]  J. Velíšek,et al.  Effects of Eugenol and MS-222 Anaesthesia on Siberian Sturgeon Acipenser baerii Brandt , 2008 .

[8]  R. Müller,et al.  The genetic consequences of hatchery-induced sperm competition in a salmonid , 2007 .

[9]  R. Eckmann,et al.  The influence of eutrophication and population biomass on common whitefish (Coregonus lavaretus) growth : the Lake Constance example revisited , 2007 .

[10]  D. Dietrich,et al.  Oral toxicity of the microcystin-containing cyanobacterium Planktothrix rubescens in European whitefish (Coregonus lavaretus). , 2006, Aquatic toxicology.

[11]  L. Novotný,et al.  Effects of Clove Oil Anaesthesia on European Catfish (Silurus glanis L.) , 2006 .

[12]  J. Heatley,et al.  CLINICAL AND CARDIORESPIRATORY EFFECTS OF PROPOFOL IN THE SPOTTED BAMBOO SHARK (CHYLLOSCYLLIUM PLAGIOSUM) , 2005, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[13]  J. Velíšek,et al.  Effects of Clove Oil Anaesthesia on Rainbow Trout (Oncorhynchus mykiss) , 2005 .

[14]  J. Velíšek,et al.  Anaesthesia of Rainbow Trout (Oncorhynchus mykiss) with 2-phenoxyethanol: Acute Toxicity and Biochemical Blood Profile , 2004 .

[15]  J. Velíšek,et al.  Anaesthesia of Common Carp ( Cyprinus carpio L.) with 2-phenoxyethanol: Acute Toxicity and Effects on Biochemical Blood Profile , 2004 .

[16]  D. Heard,et al.  EVALUATION OF PROPOFOL AND MEDETOMIDINE–KETAMINE FOR SHORT-TERM IMMOBILIZATION OF GULF OF MEXICO STURGEON (ACIPENSER OXYRINCHUS DE SOTI) , 2009, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[17]  B. J. Wicks,et al.  The effect of sub-lethal ammonia exposure on fed and unfed rainbow trout: the role of glutamine in regulation of ammonia. , 2002, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[18]  J. Lappivaara.  Effects of Acute Handling Stress on Whitefish Coregonus lavaretus After Prolonged Exposure to Biologically Treated and Untreated Bleached Kraft Mill Effluent , 2001, Archives of Environmental Contamination and Toxicology.

[19]  P. Flecknell,et al.  Laboratory animal anaesthesia , 1996 .

[20]  G. Wedemeyer.  Stress of anesthesia with M.S. 222 and Benzocaine in Rainbow Trout (Salmo gairdneri) , 1970 .