Studies on Development of Bar 68-1,a Bar-transgenic Restorer Line with Herbicide Resistance of Two-line Early Hybrid Rice

A herbicide resistant gene Bar was transferred into a two-line indica restorer line D68 by particle bombardment,and the developed transgenic restorer line Bar 68-1 and its hybrid Xiang 125S/Bar 681 showed herbicide resistance and stable inheritance based on the Southern blotting analysis and the identification of herbicide resistance.When the transgenic hybrid rice Xiang 125S/Bar 68-1 was compared with the check hybrid rice Xiang 125S/D68,the plant height and amylose content of the transgenic hybrid rice were decreased significantly at 5% and 1% levels,respectively,but all of the other major agronomic and yield characters were not statistically different from each other.