Inducibility of doxycycline-regulated gene in neural and neuroendocrine cells strongly depends on the appropriate choice of a tetracycline-responsive promoter

[1]  Guo-Yuan Yang,et al.  Comparison of doxycycline and minocycline in the inhibition of VEGF-induced smooth muscle cell migration , 2007, Neurochemistry International.

[2]  M. Gossen,et al.  Promoter crosstalk effects on gene expression. , 2007, Journal of molecular biology.

[3]  I. Petersen,et al.  An improved Tet-On regulatable FasL-adenovirus vector system for lung cancer therapy , 2006, Journal of Molecular Medicine.

[4]  M. Lupi,et al.  Tetracycline-regulated gene expression in the NSC-34-tTA cell line for investigation of motor neuron diseases. , 2005, Brain research. Molecular brain research.

[5]  L. Kaczmarek,et al.  hCMV and Tet promoters for inducible gene expression in rat neurons in vitro and in vivo , 2005, Neurobiology of Disease.

[6]  M. Lotze,et al.  Second‐generation tetracycline‐regulatable promoter: repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness , 2004, The journal of gene medicine.

[7]  Y. Chernajovsky,et al.  Endogenous GATA factors bind the core sequence of the tetO and influence gene regulation with the tetracycline system. , 2004, Molecular therapy : the journal of the American Society of Gene Therapy.

[8]  F. Kern,et al.  Homogeneity and long-term stability of tetracycline-regulated gene expression with low basal activity by using the rtTA2S-M2 transactivator and insulator-flanked reporter vectors. , 2004, Gene.

[9]  P. Bornstein,et al.  Restoration of thrombospondin 1 expression in tumor cells harbouring mutant ras oncogene by treatment with low doses of doxycycline. , 2003, Biochemical and biophysical research communications.

[10]  L. Strzadala,et al.  Early neuronal progenitor cell line expressing solely non-catalytic isoform of TrkC. , 2003, Biochemical and biophysical research communications.

[11]  W. Hillen,et al.  Gene regulation by tetracyclines. , 2003, Genetic engineering.

[12]  K. Davies,et al.  Cytotoxic effect of doxycycline and its implications for tet-on gene expression systems. , 2003, Analytical biochemistry.

[13]  Michael Litt,et al.  The insulation of genes from external enhancers and silencing chromatin , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Y. Kudo,et al.  Cell type-selective expression of green fluorescent protein and the calcium indicating protein, yellow cameleon, in rat cortical primary cultures , 2002, Brain Research.

[15]  N. Muzyczka,et al.  Comparison of the EF-1 alpha and the CMV promoter for engineering stable tumor cell lines using recombinant adeno-associated virus. , 2002, Anticancer research.

[16]  N. Daigle,et al.  A predictable ligand regulated expression strategy for stably integrated transgenes in mammalian cells in culture. , 2002, Gene.

[17]  L. Strzadala,et al.  Nurr1 affects pRL-TK but not phRG-B internal control plasmid in genetic reporter system. , 2002, Biochemical and biophysical research communications.

[18]  W. Hillen,et al.  Tetracycline-dependent gene regulation: combinations of transregulators yield a variety of expression windows. , 2002, BioTechniques.

[19]  E. Cooper,et al.  Depolarization Strongly Induces Human Cytomegalovirus Major Immediate-Early Promoter/Enhancer Activity in Neurons* , 2001, The Journal of Biological Chemistry.

[20]  C. Gatz,et al.  Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells. , 2001, Nucleic acids research.

[21]  Amyj . Williams,et al.  All in the Family: the BTB/POZ, KRAB, and SCAN Domains , 2001, Molecular and Cellular Biology.

[22]  P. Dijke,et al.  The Orphan Receptor Serine/Threonine Kinase ALK7 Signals Arrest of Proliferation and Morphological Differentiation in a Neuronal Cell Line* , 2001, The Journal of Biological Chemistry.

[23]  D. Hume,et al.  Probability in transcriptional regulation and its implications for leukocyte differentiation and inducible gene expression. , 2000, Blood.

[24]  M. T. Hasan,et al.  Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[25]  L. Kaczmarek,et al.  Efficient expression of tetracycline‐responsive gene after transfection of dentate gyrus neurons in vitro , 2000, Journal of neuroscience research.

[26]  H. Will,et al.  The tetracycline-responsive promoter contains functional interferon-inducible response elements. , 2000, Nucleic acids research.

[27]  C. Peterson,et al.  Transcriptional Regulation in Eukaryotes: Concepts, Strategies and Techniques , 2000 .

[28]  R. DePinho,et al.  Use of the human EF-1alpha promoter for expression can significantly increase success in establishing stable cell lines with consistent expression: a study using the tetracycline-inducible system in human cancer cells. , 1999, Nucleic acids research.

[29]  H. Blau,et al.  Tetracycline-regulatable factors with distinct dimerization domains allow reversible growth inhibition by p16 , 1998, Nature Genetics.

[30]  R. Evans,et al.  Inducible gene expression in mammalian cells and transgenic mice. , 1997, Current opinion in biotechnology.

[31]  M. Gossen,et al.  Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. , 1997, Nucleic acids research.

[32]  L. Journot,et al.  A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cell lines. , 1997, Nucleic acids research.

[33]  M. Gossen,et al.  Transcriptional activation by tetracyclines in mammalian cells. , 1995, Science.

[34]  U. Deuschle,et al.  Tetracycline-reversible silencing of eukaryotic promoters , 1995, Molecular and cellular biology.

[35]  S. Nagata,et al.  Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. , 1994, The Journal of biological chemistry.

[36]  M. Gossen,et al.  Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[37]  M. Gossen,et al.  Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[38]  L. Greene,et al.  Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Hermann Bujard,et al.  A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells , 1999, The journal of gene medicine.