Endothelin-1 stimulates human monocytes in vitro to release TNF-α , IL-1β and IL-6

Increased plasma- and tissue levels of endothelin-1 (ET-1) during inflammatory diseases, have suggested a role of ET-1 in the pathophysiology of inflammatory reactions. The authors have studied the effect of ET-1 on cytokine release from monocytes and monocyte-derived macrophages. ET-1 increased secretion of TNF-α, IL-1β and IL-6 in a dose- and time-dependent manner. Optimal ET-1 concentration ranged from 0.01 to 1 nM. The maximal response was a 200 to 400% increase in cytokine release. A time-course study revealed that the pattern of cytokines induced by ET-1 was different in monocytes and macrophages, although an early increase in TNF-α was observed in both monocyte and macrophage supernatants. In conclusion, ET-1 stimulates monocytes and macrophages to release cytokines thereby demonstrating a potential role for ET-1 in regulation of inflammatory responses.

[1]  A. Hauge,et al.  Endothelin-1-induced increases in microvascular permeability in isolated, perfused rat lungs requires leukocytes and plasma. , 1993, Circulatory shock.

[2]  A. Aasen,et al.  Different Role of Cytokine Mediators in Septic Shock Related to Meningococcal Disease and Surgery/Polytrauma , 1992, Immunological reviews.

[3]  M. Shichiri,et al.  Increased production of endothelin-1 in patients with inflammatory arthritides. , 1992, Arthritis and rheumatism.

[4]  W. Sessa,et al.  High endothelin-1 immunoreactivity in Crohn's disease and ulcerative colitis , 1992, The Lancet.

[5]  J. Coligan,et al.  Endothelins belong to the assortment of mast cell-derived and mast cell-bound cytokines. , 1992, The New biologist.

[6]  A. Billiau,et al.  Cytokines and their interactions with other inflammatory mediators in the pathogenesis of sepsis and septic shock , 1991, European journal of clinical investigation.

[7]  P. Suter,et al.  Elevated plasma endothelin-1 concentrations are associated with the severity of illness in patients with sepsis. , 1991, Annals of surgery.

[8]  P. Braquet,et al.  Activation of guinea pig alveolar macrophages by endothelin-1. , 1991, Journal of cardiovascular pharmacology.

[9]  R. Anderson,et al.  Endothelins, peptides with potent vasoactive properties, are produced by human macrophages , 1990, The Journal of experimental medicine.

[10]  C. Gomez-Sanchez,et al.  Hypoxia increases endothelin release in bovine endothelial cells in culture, but epinephrine, norepinephrine, serotonin, histamine and angiotensin II do not. , 1990, Life sciences.

[11]  C. Dinarello,et al.  Correlations and interactions in the production of interleukin-6 (IL-6), IL-1, and tumor necrosis factor (TNF) in human blood mononuclear cells: IL-6 suppresses IL-1 and TNF. , 1990, Blood.

[12]  J. Vilček,et al.  IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. , 1989, Journal of immunology.

[13]  K. Tracey,et al.  Antibodies to cachectin/tumor necrosis factor reduce interleukin 1 beta and interleukin 6 appearance during lethal bacteremia , 1989, The Journal of experimental medicine.

[14]  T. Inagami,et al.  Endotoxin stimulates endothelin-release in vivo and in vitro as determined by radioimmunoassay. , 1989, Biochemical and biophysical research communications.

[15]  Y. Yazaki,et al.  Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells. , 1989, Biochemical and biophysical research communications.

[16]  B. Meyrick,et al.  Human recombinant tumor necrosis factor alpha infusion mimics endotoxemia in awake sheep. , 1989, Journal of applied physiology.

[17]  J. Miao.  [Endothelium-derived contracting factors]. , 1989, Sheng li ke xue jin zhan [Progress in physiology].

[18]  W. M. Weaver,et al.  Regulation of tumor necrosis factor/cachectin and IL-1 secretion in human mononuclear phagocytes. , 1988, Journal of immunology.

[19]  Sadao Kimura,et al.  A novel potent vasoconstrictor peptide produced by vascular endothelial cells , 1988, Nature.

[20]  C. Bird,et al.  A simple sensitive bioassay for interleukin-1 which is unresponsive to 10(3) U/ml of interleukin-2. , 1987, Journal of immunological methods.

[21]  T. Espevik,et al.  A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. , 1986, Journal of immunological methods.

[22]  B. Beutler,et al.  Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1 , 1986, The Journal of experimental medicine.

[23]  R. Coffman,et al.  Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. , 1986, Journal of immunology.

[24]  J. Elias,et al.  Differential interleukin 1 elaboration by unfractionated and density fractionated human alveolar macrophages and blood monocytes: relationship to cell maturity. , 1985, Journal of immunology.

[25]  A. Bøyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. , 1968 .