STUDIES ON RABBIT LYMPHOCYTES IN VITRO

The addition of sheep antisera to rabbit IgG or to rabbit IgG subunits (L chain, H chain, Fab piece, Fc piece) to in vitro cultures of rabbit peripheral lymphocytes may induce up to 80–90% of the small lymphocytes to transform into immature "blast" cells. As many as 1 x 107 molecules of antibody may be required to stimulate each cell. It is concluded that each peripheral lymphocyte carries the antigenic specificities of the entire IgG molecule and thus could carry, and may produce specific antibody molecules. Such a conclusion supports the theory that the small lymphocyte may be the site of specific recognition of antigens in the primary response.

[1]  S. Sell.  STUDIES ON RABBIT LYMPHOCYTES IN VITRO , 1968, The Journal of experimental medicine.

[2]  S. Sell.  Use of Xenogeneic (Heterologous) Sera for Cultivation of Rabbit Lymphocytes in vitro.∗ , 1966, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[3]  N. Tomassini,et al.  ELECTRON MICROSCOPIC OBSERVATIONS ON ANTIBODY-PRODUCING CELLS IN LYMPH AND BLOOD , 1966, The Journal of experimental medicine.

[4]  R. Reisfeld,et al.  An evaluation of the number of b4 allotypic determinants on rabbit gamma-G-immunoglobulin using double-labeled, soluble complexes with univalent antibody fragments. , 1966, Immunochemistry.

[5]  R. van Furth,et al.  The formation of immunoglobulins by human tissues in vitro. IV. Circulating lymphocytes in normal and pathological conditions. , 1966, Immunology.

[6]  J. Cebra,et al.  RABBIT LYMPHOID CELLS DIFFERENTIATED WITH RESPECT TO α-, γ-, AND µ- HEAVY POLYPEPTIDE CHAINS AND TO ALLOTYPIC MARKERS AA1 AND AA2 , 1966, The Journal of experimental medicine.

[7]  S. Sell,et al.  STUDIES ON RABBIT LYMPHOCYTES IN VITRO IV. BLAST TRANSFORMATION OF THE LYMPHOCYTES FROM NEWBORN RABBITS INDUCED BY ANTIALLOTYPE SERUM TO A PATERNAL IGG ALLOTYPE NOT PRESENT IN THE SERUM OF THE LYMPHOCYTE DONORS , 1965 .

[8]  Benvenuto Pernis,et al.  CELLULAR LOCALIZATION OF IMMUNOGLOBULINS WITH DIFFERENT ALLOTYPIC SPECIFICITIES IN RABBIT LYMPHOID TISSUES , 1965, The Journal of experimental medicine.

[9]  S. Sell,et al.  STUDIES ON RABBIT LYMPHOCYTES IN VITRO : II. INDUCTION OF BLAST TRANSFORMATION WITH ANTISERA TO SIX IGG ALLOTYPES AND SUMMATION WITH MIXTURES OF ANTISERA TO DIFFERENT ALLOTYPES , 1965 .

[10]  S. Sell,et al.  STUDIES ON RABBIT LYMPHOCYTES IN VITRO : III. PROTEIN, RNA, AND DNA SYNTHESIS BY LYMPHOCYTE CULTURES AFTER STIMULATION WITH PHYTOHAEMAGGLUTININ, WITH STAPHYLOCOCCAL FILTRATE, WITH ANTIALLOTYPE SERUM, AND WITH HETEROLOGOUS ANTISERUM TO RABBIT WHOLE SERUM , 1965 .

[11]  S. Sell,et al.  STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM , 1965 .

[12]  R. Hiramoto,et al.  Detection of two antibodies in single plasma cells by the paired fluorescence technique. , 1965, Journal of immunology.

[13]  R. Reisfeld,et al.  Peptide differences of rabbit γG-immunoglobulin light chains controlled by allelic genes , 1965 .

[14]  van FURTH,et al.  [THE FORMATION OF IMMUNOGLOBULINS BY HUMAN TISSUE "IN VITRO"]. , 1964, Nederlands tijdschrift voor geneeskunde.

[15]  B. Pernis,et al.  IDENTIFICATION IN HUMAN LYMPHOID TISSUES OF CELLS THAT PRODUCE GROUP 1 OR GROUP 2 GAMMA-GLOBULINS. , 1964, Immunology.

[16]  G. Nossal,et al.  SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION , 1964, The Journal of experimental medicine.

[17]  P. Gell,et al.  Immunochemical Analysis of Rabbit Gamma-Globulin Allotypes , 1963, Nature.

[18]  P. Small,et al.  Polypeptide Chains of Rabbit Gamma Globulin , 1963, Science.

[19]  J. Fahey.  Structural Basis for the Differences between Type I and Type II Human γ-Globulin Molecules , 1963, The Journal of Immunology.

[20]  E. Sorkin,et al.  Synthesis of Antibodies by Blood Leucocytes of the Rabbit , 1963, Nature.

[21]  J. Oudin,et al.  A Notation for Allotypy , 1962, Nature.

[22]  H. A. Sober,et al.  A Simple Chromatographic Method for Preparation of Gamma Globulin.∗ , 1960, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[23]  R. Porter.  The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. , 1959, The Biochemical journal.

[24]  B. Pernis,et al.  DEMONSTRATION WITH IMMUNOFLUORESCENCE OF 19S MACROGLOBULINS AND 7S GAMMA GLOBULINS IN DIFFERENT CELLS OF THE HUMAN SPLEEN. , 1964, Pathologia et microbiologia.