Abscisic acid and mannitol promote early development, maturation and storage protein accumulation in somatic embryos of interior spruce

Low levels of mannitol (2–6%) promoted the formation of globular embryos in embryogenic cultures of interior spruce (Picea glauca engelmanni complex). However, these concentrations of mannitol were inhibitory to the formation of cotyledonary embryos. A short (1 week) pulse of mannitol in combination with abscisic acid doubled the production of late cotyledonary somatic embryos compared with the standard abscisic acid treatment. Higher levels of mannitol (13 and 20%) were required to inhibit precocious germination of spruce somatic embryos. These concentrations of mannitol promoted the accumulation of storage proteins during cotyledon maturation, but were not as effective as abscisic acid. Furthermore, 13 and 20% mannitol treatments did not substitute for abscisic acid in promoting the formation of cotyledonary embryos. Pre-treatment of late cotyledonary embryos with mannitol (13–25%) did not increase the frequency of germination compared with germination in non-treated embryos (approximately 10% germinated) although dehydration with high relative humidity treatment increased germination to 83%.

[1]  B. Flinn,et al.  Evaluation of somatic embryos of interior spruce. Characterization and developmental regulation of storage proteins , 1991 .

[2]  D. Roberts,et al.  Interaction between Maturation and High Relative Humidity Treatments and their Effects on Germination of Sitka Spruce Somatic Embryos , 1991 .

[3]  D. Webb,et al.  Storage protein changes during zygotic embryogenesis in interior spruce. , 1991, Tree physiology.

[4]  S. McInnis,et al.  Propagation of interior spruce by somatic embryogenesis. , 1990 .

[5]  M. Moloney,et al.  Effects of Abscisic Acid and High Osmoticum on Storage Protein Gene Expression in Microspore Embryos of Brassica napus. , 1990, Plant physiology.

[6]  B. Flinn,et al.  Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity , 1990 .

[7]  K. Bradford,et al.  Water Relations of Seed Development and Germination in Muskmelon (Cucumis melo L.) : III. Sensitivity of Germination to Water Potential and Abscisic Acid during Development. , 1990, Plant physiology.

[8]  D. Webb,et al.  Abscisic acid and indole‐3‐butyric acid regulation of maturation and accumulation of storage proteins in somatic embryos of interior spruce , 1990 .

[9]  M. Delseny,et al.  Effects of abscisic acid and osmotica on helianthinin gene expression in sunflower cotyledons in vitro , 1990 .

[10]  D. Webb,et al.  Factors influencing the induction of embryogenic and caulogenic callus from embryos of Piceaglauca and P. engelmanii , 1989 .

[11]  J. Bewley,et al.  Developing Seeds of Ricinus communis L., When Detached and Maintained in an Atmosphere of High Relative Humidity, Switch to a Germinative Mode without the Requirement for Complete Desiccation. , 1989, Plant physiology.

[12]  J. Bewley,et al.  The Role of Maturation Drying in the Transition from Seed Development to Germination VII. EFFECTS OF PARTIAL AND COMPLETE DESICCATION ON ABSCISIC ACID LEVELS AND SENSITIVITY IN RICINUS COMMUNIS L. SEEDS , 1989 .

[13]  C. Somerville,et al.  Abscisic acid or high osmoticum promote accumulation of long-chain fatty acids in developing embryos of Brassica napus , 1989 .

[14]  S. Arnold,et al.  Regulation of somatic embryo development in Picea abies by abscisic-acid (aba) , 1988 .

[15]  F. Bekkaoui,et al.  Effects of abscisic acid and analogues on the maturation of white spruce (Picea glauca) somatic embryos , 1988 .

[16]  K. R. Close,et al.  The effect of auxin-like plant growth regulators and osmotic regulation on induction of somatic embryogenesis from elite maize inbreds , 1987 .

[17]  K. Redenbaugh,et al.  Somatic Seeds: Encapsulation of Asexual Plant Embryos , 1986, Bio/Technology.

[18]  M. Crouch,et al.  Rapeseed embryo development in culture on high osmoticum is similar to that in seeds. , 1986, Plant physiology.

[19]  J. Bewley,et al.  The Role of Maturation Drying in the Transition from Seed Development to Germination I. ACQUISITION OF DESICCATION–TOLERANCE AND GERMINABILITY DURING DEVELOPMENT OF Ricinus communis L. SEEDS , 1985 .

[20]  S. Arnold,et al.  Plantlet regeneration through somatic embryogenesis in Picea abies (Norway spruce) , 1985 .

[21]  J. Bewley,et al.  Desiccation-Tolerant and Desiccation-Intolerant Stages during the Development and Germination of Phaseolus vulgaris Seeds , 1982 .