Nucleotide sequence organization in the very small genome of a tetraodontid fish, Arothron diadematus.

We have investigated the sequence organization of the very small genome (DNA content/haploid cell c = 0.4-0.5 pg) of a tetraodontid fish, Arotron diadematus, by using two main experimental approaches. The first one, renaturation kinetics, showed that slowly reassociating, intermediate, fast and foldback sequences represented 87%, 7%, 5% and 1%, respectively, of A. diadematus DNA, which is, so far, the vertebrate DNA lowest in repeated sequences. The second approach, centrifugation in Cs2SO4/BAMD density gradients [BAMD = bis(acetatomercurimethyl)dioxane], showed that A. diadematus DNA can be resolved into several components, characterized by buoyant densities of 1.700, 1.704(5), 1.708, 1.702 and 1.723 g/cm3, and representing 15%, 73%, 4%, 4% and 2.5%, respectively, of total DNA. The last component comprised a satellite DNA and ribosomal DNA. A family of interspersed repeats, possibly related to the AluI family of warm-blooded vertebrates, showed an extremely specific genomic distribution, being present in only the 1.708 g/cm3 component, which it matched in base composition.

[1]  Werner Arber,et al.  Genetic manipulation : impact on man and society , 1984 .

[2]  R. E. Thayer,et al.  Homology between the KpnI primate and BamH1 (M1F-1) rodent families of long interspersed repeated sequences. , 1983, Nucleic acids research.

[3]  G. Bernardi,et al.  The distribution of CR1, and Alu-like family of interspersed repeats, in the chicken genome. , 1983, Biochimica et biophysica acta.

[4]  G Bernardi,et al.  The distribution of interspersed repeats is nonuniform and conserved in the mouse and human genomes. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[5]  G. Bernardi,et al.  Organization of nucleotide sequences in the chicken genome. , 1983, European journal of biochemistry.

[6]  G. Bernardi,et al.  Sequence organization and genomic distribution of the major family of interspersed repeats of mouse DNA. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. Bernardi,et al.  The major components of the mouse and human genomes. 2. Reassociation kinetics. , 1981, European journal of biochemistry.

[8]  G. Bernardi,et al.  An analysis of fish genomes by density gradient centrifugation. , 1980, European journal of biochemistry.

[9]  G. Bernardi,et al.  THE DNA components of the chicken genome. , 1979, European journal of biochemistry.

[10]  G. Bernardi.  ORGANIZATION AND EVOLUTION OF THE EUKARYOTIC GENOME , 1979 .

[11]  G. Bernardi,et al.  Isolation and organization of calf ribosomal DNA. , 1979, Nucleic acids research.

[12]  G. Bernardi,et al.  An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl)dioxane density gradient. , 1977, European journal of biochemistry.

[13]  G Bernardi,et al.  An approach to the organization of eukaryotic genomes at a macromolecular level. , 1976, Journal of molecular biology.

[14]  G Bernardi,et al.  An analysis of eukaryotic genomes by density gradient centrifugation. , 1976, Journal of molecular biology.

[15]  G Bernardi,et al.  An analysis of the bovine genome by Cs2SO4-Ag density gradient centrifugation. , 1973, Journal of molecular biology.

[16]  R. Hinegardner,et al.  Cellular DNA Content and the Evolution of Teleostean Fishes , 1972, The American Naturalist.

[17]  C. Schmid,et al.  Sedimentation equilibrium of DNA samples heterogeneous in density , 1972, Biopolymers.

[18]  G. Bernardi,et al.  Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids. , 1968, Biochemistry.

[19]  P. Doty,et al.  The native, denatured and renatured states of deoxyribonucleic acid. , 1965, Journal of molecular biology.

[20]  J. Cairns.  The Chromosome of Escherichia coli , 1963 .

[21]  P. Doty,et al.  Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. , 1962, Journal of molecular biology.