论文信息 - Studies on the biosynthesis of epothilones: hydroxylation of Epo A and B to epothilones E and F.

Studies on the biosynthesis of epothilones: hydroxylation of Epo A and B to epothilones E and F.

When Sorangium cellulosum So ce90 is grown without XAD adsorber resin there is a steady state of epothilone A and B biosynthesis and hydroxylation of these products to epothilones E and F. This biotransformation at position C-21 of the thiazole ring is not restricted to producers of epothilones. It is carried out by a substrate induced monooxygenase. Epothilones E and F are further degraded by opening of the lactone ring by an esterase. Steps of degradation of different strains of S. cellulosum are compared.

H. Reichenbach | H. Steinmetz | K. Gerth | Gerhard Höflel

[1] H. Reichenbach,et al. Studies on the biosynthesis of epothilones: the PKS and Epothilone C/D monooxygenase. , 2001, The Journal of antibiotics.

[2] H. Reichenbach,et al. Studies on the biosynthesis of epothilones: the biosynthetic origin of the carbon skeleton. , 2000, The Journal of antibiotics.

[3] H. Reichenbach,et al. N-Oxidation of Epothilone A-C and O-Acyl Rearrangement to C-19- and C-21-Substituted Epothilones. , 1999, Angewandte Chemie.

[4] H Irschik,et al. The soraphens: a family of novel antifungal compounds from Sorangium cellulosum (Myxobacteria). I. Soraphen A1 alpha: fermentation, isolation, biological properties. , 1994, The Journal of antibiotics.

[5] J. Breittmayer,et al. Imidazole antimycotics inhibitors of cytochrome P450 increase phosphatidylserine synthesis similarly to K+‐channel blockers in Jurkat T cells , 1993, FEBS letters.