Attachment of Escherichia coli O157:H7 and Other Bacterial Cells Grown in Two Media to Beef Adipose and Muscle Tissues.

Three strains of Escherichia coli O157:H7 were grown in tryptic soy broth (TSB) or in a sterile cattle manure extract at 35°C for 18 ± 2 h. Aliquots from both inocula containing 106 CFU/ml were used to inoculate 1-cm3 cubes of beef muscle or adipose tissue by immersion for 20 min at 21°C. After removal from the inoculum, one-half of the samples were analyzed for bacterial cell numbers and pH, and the other half were stored at 4°C for 2 or 3 h before analysis. Samples were analyzed by enumerating bacteria present in liquid droplets deposited on the tissue and bacteria loosely or strongly attached to the tissue in order to determine attachment strength. Total numbers of cells on beef muscle tissue (bacteria in liquid droplets, as well as those loosely and strongly attached) were 5.65 ± 0.14 and 5.76 ± 0.26 log CFU/cm2 for E. coli O157:H7 inocula grown in TSB and manure extract, respectively. The differences in attachment strength between inocula from the two media were not significant (P > 0.05). A 2-h storage period after exposure of muscle tissue to an E. coli O157:H7 inoculum did not influence attachment strength. Numbers of bacteria attached to adipose tissue and muscle (5.31 ± 0.08 and 5.48 ± 0.09 log CFU/cm2, respectively) were not significantly different (P > 0.05). After 3 h at 4°C, the attachment strength of E. coli O157:H7 cells on muscle or adipose tissue had not changed. Overall, the culture medium and type of beef tissue did not affect the numbers of E. coli O157:H7 cells attached, nor the strength of their attachment, to muscle or adipose tissue.

[1]  T. R. Dutson,et al.  Quality Attributes and their Measurement in Meat, Poultry and Fish Products , 1995, Advances in Meat Research.

[2]  J. Frank,et al.  Bacterial starvation stress and contamination of beef , 1993 .

[3]  C. Pin,et al.  Attachment of bacteria to meat surfaces: A review. , 1993, Meat science.

[4]  M. E. Anderson,et al.  Microbiological Decontamination of Food Animal Carcasses by Washing and Sanitizing Systems: A Review. , 1992, Journal of food protection.

[5]  J. Dickson.  Attachment ofSalmonella typhimurium andListeria monocytogenes to beef tissue: effects of inoculum level, growth temperature and bacterial culture age , 1991 .

[6]  M. MacNeil,et al.  Contamination of Beef Tissue Surfaces by Cattle Manure Inoculated with Salmonella typhimurium and Listeria monocytogenes. , 1991, Journal of food protection.

[7]  R. C. Benedict,et al.  ATTACHMENT AND REMOVAL OF SALMONELLA SPP. ON MEAT AND POULTRY TISSUES , 1990 .

[8]  M. Koohmaraie,et al.  Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces , 1989, Applied and environmental microbiology.

[9]  King-Thom Chung,et al.  Attachment and Proliferation of Bacteria on Meat. , 1989, Journal of food protection.

[10]  J. Dickson.  Reduction of Bacteria Attached to Meat Surfaces by Washing with Selected Compounds. , 1988, Journal of food protection.

[11]  V. Fischetti,et al.  Variation in the expression of cell wall proteins of Staphylococcus aureus grown on solid and liquid media , 1988, Infection and immunity.

[12]  J. Farber,et al.  Attachment of Psychrotrophic Meat Spoilage Bacteria to Muscle Surfaces. , 1984, Journal of food protection.

[13]  A. Bisno,et al.  Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces , 1982, Infection and immunity.

[14]  J W Costerton,et al.  The bacterial glycocalyx in nature and disease. , 1981, Annual review of microbiology.

[15]  J. Costerton,et al.  The role of bacterial surface structures in pathogenesis. , 1981, Critical reviews in microbiology.

[16]  S. Notermans,et al.  The Effect of Cultural Procedures on the Attachment of Bacteria to Chicken Breast Meat , 1980 .

[17]  J. L. Butler,et al.  Attachment of Microorganisms to Pork Skin and Surfaces of Beef and Lamb Carcasses. , 1979, Journal of food protection.

[18]  S. Notermans,et al.  Attachment of Bacteria to Teats of Cows. , 1979, Journal of food protection.

[19]  S. Notermans,et al.  ATTACHMENT OF CERTAIN BACTERIAL STRAINS TO CHICKEN AND BEEF MEAT , 1978 .

[20]  J. Costerton,et al.  Ultrastructure of cell envelopes of bacteria of the bovine rumen. , 1975, Applied microbiology.

[21]  S. Notermans,et al.  Attachment of some bacterial strains to the skin of broiler chickens. , 1974, British poultry science.

[22]  M. Fletcher,et al.  An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces , 1973 .