Combining nanosurface chemistry and microfluidics for molecular analysis and cell biology.

Development of new tools catalyzes progress in biochemical sciences [G.M. Whitesides, E. Ostuni, S. Takayama, X.Y. Jiang, D.E. Ingber, Annual Review of Biomedical Engineering 3 (2001) 335]. Recent advances in micro-/nano-technology have resulted in an explosion of the number of new tools available for biochemical sciences. We have used surface chemistry, nano-structures and microfluidics to create a set of tools applicable for problems ranging from molecular to cellular analysis. These tools will promote the understanding of fundamental problems in cell biology, development and neurobiology, and become useful for real-world applications such as molecular diagnostics, food analysis and environmental monitoring.

[1]  Xingyu Jiang,et al.  A method for patterning multiple types of cells by using electrochemical desorption of self-assembled monolayers within microfluidic channels. , 2007, Angewandte Chemie.

[2]  Milan Mrksich,et al.  Electrochemical desorption of self-assembled monolayers noninvasively releases patterned cells from geometrical confinements. , 2003, Journal of the American Chemical Society.

[3]  Xingyu Jiang,et al.  Fabrication of Aligned Fibrous Arrays by Magnetic Electrospinning , 2007 .

[4]  P. Dutta,et al.  Hot microcontact printing for patterning ITO surfaces. Methodology, morphology, microstructure, and OLED charge injection barrier imaging , 2003 .

[5]  Donald E Ingber,et al.  Synaptic Reorganization in Scaled Networks of Controlled Size , 2007, The Journal of Neuroscience.

[6]  Wei Zheng,et al.  Highly sensitive and stable humidity nanosensors based on LiCl doped TiO2 electrospun nanofibers. , 2008, Journal of the American Chemical Society.

[7]  Mohamed Abdelgawad,et al.  All-terrain droplet actuation. , 2008, Lab on a chip.

[8]  Bon-Cheol Ku,et al.  Electrostatic Assembly of Conjugated Polymer Thin Layers on Electrospun Nanofibrous Membranes for Biosensors , 2004 .

[9]  G. Whitesides,et al.  Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane). , 2004, Langmuir : the ACS journal of surfaces and colloids.

[10]  Younan Xia,et al.  Putting Electrospun Nanofibers to Work for Biomedical Research. , 2008, Macromolecular rapid communications.

[11]  Yunyan Xie,et al.  Using azobenzene-embedded self-assembled monolayers to photochemically control cell adhesion reversibly. , 2009, Angewandte Chemie.

[12]  Xingyu Jiang,et al.  Modular microfluidics for gradient generation. , 2008, Lab on a chip.

[13]  Yang Wang,et al.  Electrospun Nanofibrous Membranes: A Novel Solid Substrate for Microfluidic Immunoassays for HIV , 2008 .

[14]  Xingyu Jiang,et al.  Palladium as a substrate for self-assembled monolayers used in biotechnology. , 2004, Analytical chemistry.

[15]  Younan Xia,et al.  Functionalization of electrospun TiO2 nanofibers with Pt nanoparticles and nanowires for catalytic applications. , 2008, Nano letters.

[16]  G. Whitesides,et al.  Experimental and theoretical scaling laws for transverse diffusive broadening in two-phase laminar flows in microchannels , 2000 .

[17]  G. Whitesides,et al.  Gradients of substrate-bound laminin orient axonal specification of neurons , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Xiao-wei Guo,et al.  Studies on the reaction between cadmium and potassium tetrahydroborate in aqueous solution and its application in atomic fluorescence spectrometry , 1995 .

[19]  K. Senecal,et al.  ELECTROSPINNING TECHNOLOGY: A NOVEL APPROACH TO SENSOR APPLICATION , 2002 .

[20]  Xingyu Jiang,et al.  A miniaturized, parallel, serially diluted immunoassay for analyzing multiple antigens. , 2003, Journal of the American Chemical Society.

[21]  C. S. Chen,et al.  Geometric control of cell life and death. , 1997, Science.

[22]  G. Whitesides,et al.  Fabrication of large-area patterned nanostructures for optical applications by nanoskiving. , 2007, Nano letters.

[23]  G. Whitesides,et al.  Microfabrication meets microbiology , 2007, Nature Reviews Microbiology.

[24]  Luke G Green,et al.  A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes. , 2002, Angewandte Chemie.

[25]  Amy Brock,et al.  Geometric determinants of directional cell motility revealed using microcontact printing. , 2003, Langmuir : the ACS journal of surfaces and colloids.

[26]  G. Whitesides,et al.  Self-assembled monolayers of thiolates on metals as a form of nanotechnology. , 2005, Chemical reviews.

[27]  Marcus Textor,et al.  An inverted microcontact printing method on topographically structured polystyrene chips for arrayed micro-3-D culturing of single cells. , 2005, Biomaterials.

[28]  R. Pepperkok,et al.  In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum , 2008, Journal of Cell Science.

[29]  Jiang Chang,et al.  Electrospinning of three-dimensional nanofibrous tubes with controllable architectures. , 2008, Nano letters.

[30]  A. Tong,et al.  New fluorescent rhodamine hydrazone chemosensor for Cu(II) with high selectivity and sensitivity. , 2006, Organic letters.

[31]  I. Mezić,et al.  Chaotic Mixer for Microchannels , 2002, Science.

[32]  George M. Whitesides,et al.  Controlling Mammalian Cell Spreading and Cytoskeletal Arrangement with Conveniently Fabricated Continuous Wavy Features on Poly(dimethylsiloxane) , 2002 .

[33]  Xingyu Jiang,et al.  Visual detection of copper(II) by azide- and alkyne-functionalized gold nanoparticles using click chemistry. , 2008, Angewandte Chemie.

[34]  Qiaobing Xu,et al.  A general method for patterning gradients of biomolecules on surfaces using microfluidic networks. , 2005, Analytical chemistry.

[35]  Hui He,et al.  A highly selective charge transfer fluoroionophore for Cu2+. , 2006, Chemical communications.

[36]  G. Whitesides,et al.  Soft lithography in biology and biochemistry. , 2001, Annual review of biomedical engineering.

[37]  G. Whitesides,et al.  New approaches to nanofabrication: molding, printing, and other techniques. , 2005, Chemical reviews.

[38]  Rong-Hua Yang,et al.  Tunable photochromism of spirobenzopyran via selective metal ion coordination: an efficient visual and ratioing fluorescent probe for divalent copper ion. , 2008, Analytical chemistry.

[39]  Xingyu Jiang,et al.  Directing cell migration with asymmetric micropatterns. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[40]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[41]  G. Wnek,et al.  Mechanical properties of electrospun fibrinogen structures. , 2006, Acta biomaterialia.