Novel Method for Neuronal Nanosurgical Connection

Neuronal injury may cause an irreversible damage to cellular, organ and organism function. While preventing neural injury is ideal, it is not always possible. There are multiple etiologies for neuronal injury including trauma, infection, inflammation, immune mediated disorders, toxins and hereditary conditions. We describe a novel laser application, utilizing femtosecond laser pulses, in order to connect neuronal axon to neuronal soma. We were able to maintain cellular viability, and demonstrate that this technique is universal as it is applicable to multiple cell types and media.

[1]  M. McBurney,et al.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns. , 1982, Developmental biology.

[2]  R. Hughes,et al.  Peripheral neuropathy. , 1982, The New England journal of medicine.

[3]  M. Rudnicki,et al.  Retinoic acid-induced neural differentiation of embryonal carcinoma cells , 1983, Molecular and cellular biology.

[4]  J. Albers,et al.  AAEM minimonograph #34: Polyneuropathy: Classification by nerve conduction studies and electromyography , 1990, Muscle & nerve.

[5]  Hannah Monyer,et al.  Electrical synapses: a dynamic signaling system that shapes the activity of neuronal networks. , 2004, Biochimica et biophysica acta.

[6]  E. Rosenzweig,et al.  Rodent models for treatment of spinal cord injury: research trends and progress toward useful repair , 2004, Current opinion in neurology.

[7]  L. Robinson.  traumatic injury to peripheral nerves. , 2005, Supplements to Clinical neurophysiology.

[8]  B. Dobkin,et al.  Basic advances and new avenues in therapy of spinal cord injury. , 2004, Annual review of medicine.

[9]  M. Bennett,et al.  Electrical Coupling and Neuronal Synchronization in the Mammalian Brain , 2004, Neuron.

[10]  Vikram Kohli,et al.  Cell nanosurgery using ultrashort (femtosecond) laser pulses: Applications to membrane surgery and cell isolation , 2005, Lasers in surgery and medicine.

[11]  A. Y. Elezzabi,et al.  Reversible permeabilization using high-intensity femtosecond laser pulses: applications to biopreservation. , 2005, Biotechnology and bioengineering.

[12]  Richard S Nowakowski,et al.  Stable neuron numbers from cradle to grave , 2006, Proceedings of the National Academy of Sciences.

[13]  M Leonor Cancela,et al.  An alternative method for delivering exogenous material into developing zebrafish embryos , 2007, Biotechnology and bioengineering.

[14]  Vikram Kohli,et al.  Laser surgery of zebrafish (Danio rerio) embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the laser's effect on short- and long-term development , 2008, BMC biotechnology.

[15]  A. L. Amaral,et al.  Quantification of the CBD-FITC conjugates surface coating on cellulose fibres , 2008, BMC biotechnology.

[16]  Abdulhakem Y. Elezzabi,et al.  Prospects and developments in cell and embryo laser nanosurgery. , 2009, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[17]  Digant P. Dave,et al.  Neuro-optical microfluidic platform to study injury and regeneration of single axons. , 2009, Lab on a chip.

[18]  Masahito Yamagata,et al.  Many paths to synaptic specificity. , 2009, Annual review of cell and developmental biology.

[19]  M. Freeman,et al.  Wallerian degeneration, wld(s), and nmnat. , 2010, Annual review of neuroscience.

[20]  B. Barres,et al.  Axon degeneration: Molecular mechanisms of a self-destruction pathway , 2012, The Journal of cell biology.

[21]  B. Barres,et al.  Axon Degeneration: Where the Wlds Things Are , 2012, Current Biology.

[22]  Nir Katchinskiy,et al.  Femtosecond laser‐induced cell‐cell surgical attachment , 2014, Lasers in surgery and medicine.

[23]  Bill Bynum,et al.  Lancet , 2015, The Lancet.