Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat

Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.

[1]  Anna Maria Dewajanti.  Peranan Asam Klorogenat Tanaman Kopi Terhadap Penurunan Kadar Asam Urat dan Beban Oksidatif , 2019, Jurnal Kedokteran Meditek.

[2]  D. Spandidos,et al.  Comparison of antioxidant activity between green and roasted coffee beans using molecular methods , 2015, Molecular medicine reports.

[3]  A. Alalwani.  Monosodium glutamate induced testicular lesions in rats (histological study) , 2014 .

[4]  Tri Retnaninggsih Soeprobowati,et al.  POTENSI TEH HIJAU (Camelia sinensis L.) DALAM PERBAIKAN FUNGSI HEPAR PADA MENCIT YANG DIINDUKSI MONOSODIUM GLUTAMAT (MSG) , 2012 .

[5]  Onyema Oscar Okwudiri.  Monosodium Glutamate Induces Oxidative Stress and Affects Glucose Metabolism in the Kidney of Rats , 2012 .

[6]  S. Maya.  Gangguan Perkembangan Testis Dan Penurunan Kadar Testosteron Pada Hewan Coba Akibat Paparan Monosodium Glutamate (MSG) Yang Berlebihan , 2010 .

[7]  Megawati Eka Roina.  Penurunan Jumlah Sperma Hewan Coba Akibat Paparan Monosodium Glutamate , 2010 .

[8]  Lien Ai Pham-Huy,et al.  Free Radicals, Antioxidants in Disease and Health , 2008, International journal of biomedical science : IJBS.

[9]  A. O. Eweka.  Histological studies of the effects of monosodium glutamate on the kidney of adult Wistar rats , 2006 .

[10]  T. Labuza,et al.  Essentials Of Functional Foods , 2000 .

[11]  P. Yarnold,et al.  Review of alleged reaction to monosodium glutamate and outcome of a multicenter double-blind placebo-controlled study. , 2000, The Journal of nutrition.

[12]  Y. Chu.  Coffee: emerging health effects and disease prevention. , 2012 .

[13]  Neveen,et al.  Evaluation of Antioxidant Effect of Nigella sativa oil on Monosodium Glutamate-Induced Oxidative Stress in Rat Brain , 2010 .