Effects of deoxynivalenol and zearalenone on oxidative stress and blood phagocytic activity in broilers

Effects of dietary contamination with various levels of deoxynivalenol (DON) and zearalenone (ZEA) were investigated on Ross 308 hybrid broilers of both sexes. After hatching, all chickens were fed an identical control diet for two weeks. Then chickens of Group 1 received a diet contaminated with DON and ZEA, both being 3.4 mg · kg−1, while Group 2 received DON and ZEA at 8.2 and 8.3 mg · kg−1, respectively. The diet of the control group contained background levels of mycotoxins. Samples of blood and tissues were collected after two weeks. Intake of both contaminated diets resulted in a significantly decreased activity of glutathione peroxidase (GPx) and increased level of malondialdehyde (MDA) in liver tissue, while in kidneys the concentration of MDA was significantly increased only in Group 1. On the other hand, activities of blood GPx and plasma γ-glutamyltransferase (GGT) were elevated in Group 2 only. Activities of thioredoxin reductase in liver and GPx in duodenal mucosa tissues, superoxide dismutase (SOD) in erythrocytes as well as levels of MDA in duodenal mucosa and α-tocopherol in plasma were not affected by dietary mycotoxins. Blood phagocytic activity was significantly depressed in Group 1 and 2. These results demonstrate that diets contaminated with DON and ZEA at medium levels are already able to induce oxidative stress and compromise the blood phagocytic activity in fattening chickens.

[1]  Peter F Surai,et al.  Protective effect of modified glucomannans and organic selenium against antioxidant depletion in the chicken liver due to T-2 toxin-contaminated feed consumption. , 2007, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[2]  J. Fink-Gremmels,et al.  Species differences in the hepatic biotransformation of zearalenone. , 2006, Veterinary journal.

[3]  J. Zentek,et al.  Effects of feeding deoxynivalenol contaminated wheat on growth performance, organ weights and histological parameters of the intestine of broiler chickens. , 2006, Journal of animal physiology and animal nutrition.

[4]  J. Pestka,et al.  Deoxynivalenol: Toxicology and Potential Effects on Humans , 2005, Journal of toxicology and environmental health. Part B, Critical reviews.

[5]  R. Krska,et al.  Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry. , 2005, Journal of agricultural and food chemistry.

[6]  I. Oswald,et al.  Immunotoxicological risk of mycotoxins for domestic animals , 2005, Food additives and contaminants.

[7]  D. Jacobs,et al.  Is serum gamma-glutamyltransferase inversely associated with serum antioxidants as a marker of oxidative stress? , 2004, Free radical biology & medicine.

[8]  F. Minervini,et al.  Toxicity and apoptosis induced by the mycotoxins nivalenol, deoxynivalenol and fumonisin B1 in a human erythroleukemia cell line. , 2004, Toxicology in vitro : an international journal published in association with BIBRA.

[9]  K. Becker,et al.  The thioredoxin system—From science to clinic , 2004, Medicinal research reviews.

[10]  G. Flachowsky,et al.  Effects of graded levels of Fusarium toxin contaminated maize in diets for female weaned piglets , 2003, Archiv fur Tierernahrung.

[11]  R. Labuda,et al.  Identification and enumeration of Fusarium species in poultry feed mixtures from Slovakia. , 2003, Annals of agricultural and environmental medicine : AAEM.

[12]  Peter F Surai.  Selenium in poultry nutrition 1. Antioxidant properties, deficiency and toxicity , 2002 .

[13]  D. Hatfield,et al.  "Selenium: Its Molecular Biology and Role in Human Health" , 2002 .

[14]  G. Flachowsky,et al.  Excretion kinetics and metabolism of zearalenone in broilers in dependence on a detoxifying agent , 2001, Archiv fur Tierernahrung.

[15]  J. Pestka,et al.  Immunomodulation by fungal toxins. , 2000, Journal of toxicology and environmental health. Part B, Critical reviews.

[16]  A. Pietri,et al.  Evaluation of a fluorodensitometric method for analysis of ergosterol as a fungal marker in compound feeds. , 1999, Journal of food protection.

[17]  D. Ahn,et al.  Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. , 1998, Poultry science.

[18]  J. Pestka,et al.  Toxicology of deoxynivalenol (vomitoxin). , 1996, Journal of toxicology and environmental health.

[19]  M. Ahotupa,et al.  Protective effect of antioxidants against free radical-mediated lipid peroxidation induced by DON or T-2 toxin. , 1994, Zentralblatt fur Veterinarmedizin. Reihe A.

[20]  A. Ayral,et al.  In vitro effect of diacetoxyscirpenol and deoxynivalenol on microbicidal activity of murine peritoneal macrophages , 1992, Mycopathologia.

[21]  M. A. Qureshi,et al.  Effects of AFB1 embryonic exposure on chicken mononuclear phagocytic cell functions. , 1992, Developmental and comparative immunology.

[22]  D. Corrier.  Mycotoxicosis: mechanisms of immunosuppression. , 1991, Veterinary immunology and immunopathology.

[23]  A. Rizzo,et al.  Investigation on Trichothecene-Stimulated Lipid Peroxidation and Toxic Effects of Trichothecenes in Animals , 1989, Acta Veterinaria Scandinavica.

[24]  J. Miller,et al.  Tissue distribution and excretion of radioactivity following administration of 14C-labeled deoxynivalenol to White Leghorn hens. , 1986, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[25]  J. Arthur,et al.  Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. , 1985, Life sciences.

[26]  L. Kašpárek,et al.  Phagocytosis of human blood leukocytes: a simple micromethod. , 1982, Immunology letters.

[27]  G. Combs,et al.  Impaired vitamin E status of chicks fed T-2 toxin. , 1981, Poultry science.

[28]  P. Hamilton,et al.  Impairment of phagocytosis by heterophils from chickens during ochratoxicosis , 1980, Applied and environmental microbiology.

[29]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[30]  G. Szász.  A Kinetic Photometric Method for Serum γ-Glutamyl Transpeptidase , 1969 .

[31]  W. Valentine,et al.  Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. , 1967, The Journal of laboratory and clinical medicine.

[32]  Peter F Surai,et al.  Selenium in poultry nutrition. , 2008 .

[33]  R. Brigelius-Flohé,et al.  Selenoproteins of the glutathione system , 2006 .

[34]  P. Guerre,et al.  Production and purification of fumonisins from a highly toxigenic Fusarium verticilloides strain , 2005 .

[35]  M. Mézes,et al.  Effect of excess selenium supplementation on the glutathione redox system in broiler chicken. , 2004, Acta veterinaria Hungarica.

[36]  M. Gajęcki.  Zearalenone--undesirable substances in feed. , 2002, Polish journal of veterinary sciences.

[37]  M. Mézes,et al.  Comparative investigation on the effect of T-2 mycotoxin on lipid peroxidation and antioxidant status in different poultry species. , 1999, Research in veterinary science.

[38]  A. Holmgren,et al.  Thioredoxin and thioredoxin reductase. , 1995, Methods in enzymology.

[39]  A. Holmgren,et al.  [21] Thioredoxin and thioredoxin reductase , 1995 .

[40]  H. R. Burmeister,et al.  Pharmacokinetics of the trichothecene mycotoxin, T-2 toxin, in swine and cattle. , 1986, Toxicon : official journal of the International Society on Toxinology.