Bioinformatics Analysis of the Interaction between Coat Protein and Nuclear Shuttle Protein in Babuvirus

Protein and protein interactions play important roles in many biological processes and are responsible for carrying out the function of biological regulatory network in living organisms. Previous study indicated that Banana bunchy top virus (BBTV) coat protein (CP) interacted with BBTV nuclear shuttle protein (NSP). However, the protein and protein interaction and the binding affinity of CP and NSP in Babuvirus are remaining unclear. In this study, the CPs and NSPs proteins of BBTV, Abaca bunchy top virus (ABTV) and Cardamom bushy dwarf virus (CBDV) were used for bioinformatic analysis. The binding free energy and the dissociation constant of the possible interaction proteins were tested in PPA-Pred2, and the results confirmed CP interaction with NSP in Babuvirus. The study will help us to understand the interaction between viral protein and viral protein, and the pathogenesis mechanism of Babuvirus in host plants.

[1]  Bin-bin Li,et al.  Banana bunchy top virus (BBTV) nuclear shuttle protein interacts and re-distributes BBTV coat protein in Nicotiana benthamiana , 2019, 3 Biotech.

[2]  Geng Wu,et al.  Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro. , 2019, Methods in molecular biology.

[3]  Ning Gan,et al.  A two dimensional metal-organic framework nanosheets-based fluorescence resonance energy transfer aptasensor with circular strand-replacement DNA polymerization target-triggered amplification strategy for homogenous detection of antibiotics. , 2018, Analytica chimica acta.

[4]  T. Lin,et al.  Affinity capture surface carboxyl-functionalized MoS2 sheets to enhance the sensitivity of surface plasmon resonance immunosensors. , 2018, Talanta.

[5]  E. Flemington,et al.  Transferring knowledge of bacterial protein interaction networks to predict pathogen targeted human genes and immune signaling pathways: a case study on M. tuberculosis , 2018, BMC Genomics.

[6]  Elliot J. Lefkowitz,et al.  Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV) , 2017, Nucleic Acids Res..

[7]  U. Sonnewald,et al.  The stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus. , 2017, Virus research.

[8]  D. Collings,et al.  Comparative analysis of common regions found in babuviruses and alphasatellite molecules , 2017, Archives of Virology.

[9]  J. Trinidad,et al.  Disparate Regulatory Mechanisms Control Fat3 and P75NTR Protein Transport through a Conserved Kif5-Interaction Domain , 2016, PloS one.

[10]  M. Chabannes,et al.  Biology, etiology, and control of virus diseases of banana and plantain. , 2015, Advances in virus research.

[11]  J. Qazi,et al.  Banana bunchy top virus and the bunchy top disease , 2015, Journal of General Plant Pathology.

[12]  A. Bressan,et al.  Tropism, compartmentalization and retention of banana bunchy top virus (Nanoviridae) in the aphid vector Pentalonia nigronervosa. , 2013, The Journal of general virology.

[13]  Zhi-xin Liu,et al.  Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan , 2012, Virus Genes.

[14]  Zhi-xin Liu,et al.  Bioinformatic analysis of BBTV satellite DNA in Hainan , 2011, Virologica Sinica.

[15]  A. Bressan,et al.  Immunofluorescence localisation of Banana bunchy top virus (family Nanoviridae) within the aphid vector, Pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses. , 2011, Virus research.

[16]  Y. Nai.  New research advance of banana bunchy top virus , 2011 .

[17]  Xianbing Wang,et al.  Identification of two RNA silencing suppressors from banana bunchy top virus , 2009, Archives of Virology.

[18]  Christopher A. Voigt,et al.  Spatiotemporal Control of Cell Signalling Using A Light-Switchable Protein Interaction , 2009, Nature.

[19]  B. Gronenborn.  Nanoviruses: genome organisation and protein function. , 2004, Veterinary microbiology.

[20]  J. Dale,et al.  Functional analysis of proteins encoded by banana bunchy top virus DNA-4 to -6. , 2000, The Journal of general virology.

[21]  J. Dale,et al.  Banana bunchy top virus DNA-3encodes the viral coat protein , 1997, Archives of Virology.

[22]  J. Dale,et al.  The genome organization of banana bunchy top virus: analysis of six ssDNA components. , 1995, The Journal of general virology.