Reduced Platelet Adhesion on the Surface of Polyurethane Bearing Structure of Sulfobetaine

Poly(etherurethane)s are widely used as blood-contacting biomaterials due to their good biocompatibility and mechanical properties. Nevertheless, their blood compatibility is still not adequate for the more demanding applications. Surface modification is an effective way to improve the blood compatibility and retain the bulk properties of biomaterials. The purpose of present study was to design and synthesize a novel nonthrombogenic biomaterial by modifying the surface of poly(etherurethane) with zwitterionic monomer. Films of polyurethane were grafted with sulfobetaine by a three-step procedure. In the first step, the film surfaces were treated with hexamethylene diisocyanate (HDI) in toluene at 50 C in the presence of di-n-butyl tin dilaurate (DBTDL) as a catalyst. The extent of the reaction was measured by ATR-IR spectra; a maximum number of free NCO group was obtained after a reaction time of 90 min. In the second step, the hydroxyl group of 4-dimethylamino-1-butanol (DMAB) was allowed to react in toluene with isocyanate groups bound on the surface. In the third step, sulfobetaine was formed on the surface through the ring-opening reaction between tertiary amine of DMAB and 1,3- propanesultone (PS). It was characterized by ATR-IR, XPS. The data showed that the grafted surfaces were composed of sulfobetaine. The results of the contact angle measurements showed that they were strongly hydrophilic. The state of platelet adhesion and shape variation for the attached platelets was described. The modified surface shows excellent blood compatibility feature by the low platelet adhesion.

[1]  Jian Shen,et al.  Grafting sulfobetaine monomer onto silicone surface to improve haemocompatibility , 2004 .

[2]  Jiang Yuan,et al.  Chemical grafting of sulfobetaine onto poly(ether urethane) surface for improving blood compatibility , 2003 .

[3]  Jiang Yuan,et al.  Platelet adhesive resistance of segmented polyurethane film surface-grafted with vinyl benzyl sulfo monomer of ammonium zwitterions. , 2003, Biomaterials.

[4]  W. Zhub,et al.  Grafting of sulfobetaine onto a polyurethane surface to improve blood compatibility , 2003 .

[5]  Jiang Yuan,et al.  Improvement of blood compatibility on cellulose membrane surface by grafting betaines , 2003 .

[6]  Z. Jun,et al.  Surface modification of segmented poly(ether urethane) by grafting sulfo ammonium zwitterionic monomer to improve hemocompatibilities , 2003 .

[7]  S. Kim,et al.  PEO-grafting on PU/PS IPNs for enhanced blood compatibility--effect of pendant length and grafting density. , 2002, Biomaterials.

[8]  J. Zhang,et al.  Grafting sulfobetaine monomer onto the segmented poly(ether-urethane) surface to improve hemocompatibility , 2002, Journal of biomaterials science. Polymer edition.

[9]  Linxu Si.  Synthesis Studies on Blood Compatible Materials(VII)──A Novel Kind of Platelet Adhesion Resistant Polymers , 2002 .

[10]  Y. Takemoto,et al.  Synthesis, characterization and platelet adhesion of segmented polyurethanes grafted phospholipid analogous vinyl monomer on surface. , 2002, Biomaterials.

[11]  J. C. Lin,et al.  Surface characterization and platelet adhesion studies on polyethylene surface with hirudin immobilization , 2001, Journal of materials science. Materials in medicine.

[12]  J. Ji,et al.  Surface coating of stearyl poly(ethylene oxide) coupling-polymer on polyurethane guiding catheters with poly(ether urethane) film-building additive for biomedical applications. , 2001, Biomaterials.

[13]  D. Liaw,et al.  Macromolecular microstructure, reactivity ratio and viscometric studies of water-soluble cationic and/or zwitterionic copolymers , 2000 .

[14]  Ruifeng Zhang,et al.  Anticoagulant surface prepared by the heparinization of ionic polyurethane film , 2000 .

[15]  D. M. Kim,et al.  Platelet adhesion onto segmented polyurethane film surfaces modified by addition and crosslinking of PEO-containing block copolymers. , 2000, Biomaterials.

[16]  K. Ishihara,et al.  Chemical modification of silk fibroin with 2-methacryloyloxyethyl phosphorylcholine. II. Graft-polymerization onto fabric through 2-methacryloyloxyethyl isocyanate and interaction between fabric and platelets. , 2000, Biomaterials.

[17]  Y. Bae,et al.  PDMS-based polyurethanes with MPEG grafts: synthesis, characterization and platelet adhesion study. , 1999, Biomaterials.

[18]  I. Kang,et al.  Synthesis and characterization of heparinized polyurethanes using plasma glow discharge. , 1999, Biomaterials.

[19]  H. Suh,et al.  Bacterial adhesion on PEG modified polyurethane surfaces. , 1998, Biomaterials.

[20]  N Nakabayashi,et al.  Platelet adhesion on the gradient surfaces grafted with phospholipid polymer. , 1998, Journal of biomaterials science. Polymer edition.

[21]  M. Kodama,et al.  Blood Compatible Phospholipid-Containing Polyurethanes: Synthesis, Characterization and Blood Compatibility Evaluation , 1997, Journal of biomaterials applications.

[22]  B. Wesslén,et al.  Grafting of Polyurethane Surfaces with Poly(Ethylene Glycol) , 1993 .

[23]  Luis C. Salazar,et al.  Water soluble copolymers: 46. Hydrophilic sulphobetaine copolymers of acrylamide and 3-(2-acrylamido-2-methylpropanedimethylammonio)-1-propanesulphonate , 1992 .

[24]  T. Okano,et al.  Synthesis and characterization of SPUU–PEO–heparin graft copolymers , 1991 .

[25]  D. Liaw,et al.  Aqueous solution properties of poly[3‐dimethyl (methacryloyloxyethyl) ammonium propane sulfonate] , 1987 .

[26]  D. Liaw,et al.  Thermal degradation of poly [3-dimethyl(methylmethacryloylethyl) ammonium propanesulfonate] , 1985 .

[27]  V. Soto,et al.  Poly(sulphopropylbetaines): 2. Dilute solution properties , 1984 .

[28]  V. Soto,et al.  Poly(sulphopropylbetaines): 1. Synthesis and characterization , 1984 .

[29]  S. Israel,et al.  Preparation of inner salt polymers from vinylimidazolium sulphobetaines , 1977 .