[Application of synthetic biology in environmental remediation].

Environmental problems are the most serious challenges in the 21st century. With the rapid development of modern industry and agriculture, ecological and environmental deterioration have become the most important factors to restrict the sustainable development of social economy. Microbial cells have strong ability for environmental remediation, but their evolution speed is slower than the speed of emerging pollutants. Therefore, the treatment using the synthetic biology is in urgent need. Full understanding of the microbial degradation characteristics (pathways) of refractory organic pollutants with the help of abundant microbial and gene resources in China is important. Using synthetic biology to redesign and transform the existing degrading strain will be used to degrade particular organic pollutants or multiple organic pollutants. For the complex pollutants, such as wastewater, based on the establishment of metabolic or regulation or resistance related gene modules of typical organic pollutants, artificial flora could be designed to solve the complex pollutants. The rational design and construction of engineering bacteria for typical environmental organic pollutants can effectively promote microbial catabolism of emerging contaminants, providing technical support for environmental remediation in China.

[1]  V. de Lorenzo,et al.  High-resolution analysis of the m-xylene/toluene biodegradation subtranscriptome of Pseudomonas putida mt-2. , 2016, Environmental microbiology.

[2]  J. Nougayrède,et al.  The Bacterial Stress-Responsive Hsp90 Chaperone (HtpG) Is Required for the Production of the Genotoxin Colibactin and the Siderophore Yersiniabactin in Escherichia coli. , 2016, The Journal of infectious diseases.

[3]  Rebecca E Parales,et al.  Direct sensing and signal transduction during bacterial chemotaxis toward aromatic compounds in Comamonas testosteroni , 2016, Molecular microbiology.

[4]  Chuan-peng Liu,et al.  Large-scale gene expression profiling reveals physiological response to deletion of chaperone dnaKJ in Escherichia coli. , 2016, Microbiological research.

[5]  R. Jáuregui,et al.  Degradation of Benzene by Pseudomonas veronii 1YdBTEX2 and 1YB2 Is Catalyzed by Enzymes Encoded in Distinct Catabolism Gene Clusters , 2015, Applied and Environmental Microbiology.

[6]  J. Shiloach,et al.  Constitutive expression of the sRNA GadY decreases acetate production and improves E. coli growth , 2015, Microbial Cell Factories.

[7]  杨颖,et al.  Occurrence and potential sources of antibiotics in Beijiang River, Southern China , 2015 .

[8]  Z. Shao,et al.  The long-chain alkane metabolism network of Alcanivorax dieselolei , 2014, Nature Communications.

[9]  Wanchen Li,et al.  Heterologous expression of betaine aldehyde dehydrogenase gene from Ammopiptanthus nanus confers high salt and heat tolerance to Escherichia coli. , 2014, Gene.

[10]  Yanping Zhang,et al.  Engineering cellular robustness of microbes by introducing the GroESL chaperonins from extremophilic bacteria. , 2014, Journal of biotechnology.

[11]  Sun Xiangyin.  Construction of heat resistance devices for Escherichia coli and their application , 2014 .

[12]  Shuangjiang Liu,et al.  Comamonas testosteroni uses a chemoreceptor for tricarboxylic acid cycle intermediates to trigger chemotactic responses towards aromatic compounds , 2013, Molecular microbiology.

[13]  P. Xu,et al.  Systematic Unraveling of the Unsolved Pathway of Nicotine Degradation in Pseudomonas , 2013, PLoS genetics.

[14]  Song-He Wang,et al.  Biochemical and Molecular Characterization of the Gentisate Transporter GenK in Corynebacterium glutamicum , 2012, PloS one.

[15]  Lv Xiao-ming.  Research Progress of Endocrine Disrupting Chemicals(EDCs) and Pharmaceuticals and Personal Care Products(PPCPs) , 2012 .

[16]  W. Min.  Experimental Study on Microbial Degradation of Tetracycline Residues in Antibiotic Waste , 2012 .

[17]  Rong Li,et al.  A Novel Hydrolytic Dehalogenase for the Chlorinated Aromatic Compound Chlorothalonil , 2010, Journal of bacteriology.

[18]  Z. Deng,et al.  New metabolites in dibenzofuran cometabolic degradation by a biphenyl-cultivated Pseudomonas putida strain B6-2. , 2009, Environmental science & technology.

[19]  P. Leinweber,et al.  Distribution of sulfamethazine, chlortetracycline and tylosin in manure and soil of Canadian feedlots after subtherapeutic use in cattle. , 2008, Environmental pollution.

[20]  H. Strahl,et al.  The extremely halophilic archaeon Halobacterium salinarum R1 responds to potassium limitation by expression of the K+-transporting KdpFABC P-type ATPase and by a decrease in intracellular K+ , 2008, Extremophiles.

[21]  Z. Qixing.  Research Advancement of Eco-toxicity of Tetracycline Antibiotics , 2008 .

[22]  P. Goffin,et al.  Improved adaptation to heat, cold, and solvent tolerance in Lactobacillus plantarum , 2007, Applied Microbiology and Biotechnology.

[23]  Shuangjiang Liu,et al.  Genome-wide investigation of aromatic acid transporters in Corynebacterium glutamicum. , 2007, Microbiology.

[24]  M. Fukuda,et al.  Multiple-Subunit Genes of the Aromatic-Ring-Hydroxylating Dioxygenase Play an Active Role in Biphenyl and Polychlorinated Biphenyl Degradation in Rhodococcus sp. Strain RHA1 , 2006, Applied and Environmental Microbiology.

[25]  Hong Liu,et al.  Conversion of Sphingobium chlorophenolicum ATCC 39723 to a Hexachlorobenzene Degrader by Metabolic Engineering , 2006, Applied and Environmental Microbiology.

[26]  B. Zhang,et al.  A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8T: cloning and molecular characterization. , 2006, FEMS microbiology letters.

[27]  Yuji Nagata,et al.  Degradation of β-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26 , 2005, Applied and Environmental Microbiology.

[28]  Ting-Wei Chen,et al.  [Construction of fused osmoregulation proBA gene from a salt-tolerant mutant of Bacillus subtilis and its influence on the osmotolerance of Escherichia coli]. , 2005, Wei sheng wu xue bao = Acta microbiologica Sinica.

[29]  Zhang Fu-dao.  Determination and analysis on main harmful composition in excrement of scale livestock and poultry feedlots , 2005 .

[30]  Chengjun Sun,et al.  Determination of environmental estrogens in human urine by high performance liquid chromatography after fluorescent derivatization with p-nitrobenzoyl chloride , 2004 .

[31]  S. Copley,et al.  Genome Shuffling Improves Degradation of the Anthropogenic Pesticide Pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723 , 2004, Applied and Environmental Microbiology.

[32]  Tan Dian-xiang.  Determination of diethylstilbestrol in animal tissues by HPLC/ECD , 2004 .

[33]  H. Z. Ninnekar,et al.  Biodegradation of DDT by a Pseudomonas Species , 2003, Current Microbiology.

[34]  L. Xun,et al.  Organization and Regulation of Pentachlorophenol-Degrading Genes in Sphingobium chlorophenolicum ATCC 39723 , 2002, Journal of bacteriology.

[35]  T. Omori,et al.  Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome. , 2002, Biochemical and biophysical research communications.

[36]  H. Nau,et al.  Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. , 2002, Analytical chemistry.

[37]  A. Ohta,et al.  Expression of the bph genes involved in biphenyl/PCB degradation in Pseudomonas sp. KKS102 induced by the biphenyl degradation intermediate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid. , 2000, Gene.

[38]  Antonio Ventosa,et al.  Biology of Moderately Halophilic Aerobic Bacteria , 1998, Microbiology and Molecular Biology Reviews.

[39]  D. Gibson,et al.  Isolation and preliminary characterization of the subunits of the terminal component of naphthalene dioxygenase from Pseudomonas putida NCIB 9816-4 , 1993, Journal of bacteriology.

[40]  Li Jie,et al.  Introduction of Environmental Endocrine Disruptors , 1993 .

[41]  F. Mondello,et al.  Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400 , 1992, Journal of bacteriology.

[42]  D. Gibson,et al.  Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli. , 1989, The Journal of biological chemistry.

[43]  L N Csonka,et al.  Physiological and genetic responses of bacteria to osmotic stress. , 1989, Microbiological reviews.