The cellulosome--a treasure-trove for biotechnology.

The cellulases of many cellulolytic bacteria are organized into discrete multienzyme complexes, called cellulosomes. The multiple subunits of cellulosomes are composed of numerous functional domains, which interact with each other and with the cellulosic substrate. One of these subunits comprises a distinctive new class of noncatalytic scaffolding polypeptide, which selectively integrates the various cellulase and xylanase subunits into the cohesive complex. Intelligent application of cellulosome hybrids and chimeric constructs of cellulosomal domains should enable better use of cellulosic biomass and may offer a wide range of novel applications in research, medicine and industry.

[1]  嶋田 協,et al.  Genetics, biochemistry and ecology of lignocellulose degradation , 1992 .

[2]  P Béguin,et al.  Molecular biology of cellulose degradation. , 1990, Annual review of microbiology.

[3]  M. Coughlan,et al.  beta‐1,4‐D‐xylan‐degrading enzyme systems: biochemistry, molecular biology and applications , 1993, Biotechnology and applied biochemistry.

[4]  Tuula T. Teeri,et al.  Cellulase families and their genes , 1987 .

[5]  J. Greenwood,et al.  Cellulose-binding domains: potential for purification of complex proteins. , 1992, Protein engineering.

[6]  E. Bayer,et al.  Specialized cell surface structures in cellulolytic bacteria , 1987, Journal of bacteriology.

[7]  F. Guerlesquin,et al.  Purification and characterization of endoglucanase C from Clostridium cellulolyticum. Catalytic comparison with endoglucanase A. , 1993, European journal of biochemistry.

[8]  Raphael Lamed,et al.  The Cellulosome of Clostridium thermocellum , 1988 .

[9]  Christos Stathopoulos,et al.  Specific Adhesion and Hydrolysis of Cellulose by Intact Escherichia coli Expressing Surface Anchored Cellulase or Cellulose Binding Domains , 1993, Bio/Technology.

[10]  D. Kilburn,et al.  Enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein. , 1991, Enzyme and microbial technology.

[11]  J. Aubert,et al.  The biological degradation of cellulose. , 1994, FEMS microbiology reviews.

[12]  T. Teeri,et al.  An active single-chain antibody containing a cellulase linker domain is secreted by Escherichia coli. , 1991, Protein engineering.

[13]  H. Gilbert,et al.  Characterization of hybrid proteins consisting of the catalytic domains of Clostridium and Ruminococcus endoglucanases, fused to Pseudomonas non-catalytic cellulose-binding domains. , 1991, The Biochemical journal.

[14]  Michael P. Coughlan,et al.  Macromolecular Organization of the Cellulolytic Enzyme Complex of Clostridium thermocellum as Revealed by Electron Microscopy , 1987, Applied and environmental microbiology.

[15]  J. Aubert,et al.  Involvement of separate domains of the cellulosomal protein S1 of Clostridium thermocellum in binding to cellulose and in anchoring of catalytic subunits to the cellulosome , 1992, FEBS letters.

[16]  A Bairoch,et al.  New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. , 1993, The Biochemical journal.

[17]  D. Kilburn,et al.  A Bifunctional Affinity Linker to Couple Antibodies to Cellulose , 1993, Bio/Technology.

[18]  Cheorl-Ho Kim,et al.  Extracellular cellulolytic enzymes ofBacillus circulans are present as two multipleprotein complexes , 1993 .

[19]  E. Bayer,et al.  Ultrastructure of the cell surface cellulosome of Clostridium thermocellum and its interaction with cellulose , 1986, Journal of bacteriology.

[20]  P. Dhurjati,et al.  Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components , 1991, FEBS letters.

[21]  R. Doi,et al.  The hydrophobic repeated domain of the Clostridium cellulovorans cellulose-binding protein (CbpA) has specific interactions with endoglucanases , 1993, Journal of bacteriology.

[22]  J. Greenwood,et al.  The cellulose-binding domains of cellulases: tools for biotechnology , 1989 .

[23]  S. Karita,et al.  Nucleotide sequences of the celB gene encoding endo-1,4-β-β-glucanase-2, ORF1 and ORF2 forming a putative cellulase gene cluster of Clostridium josui , 1993 .

[24]  E. Bayer,et al.  Unorthodox intrasubunit interactions in the cellulosome ofClostridium thermocellum , 1992 .

[25]  Harry J. Gilbert,et al.  Bacterial cellulases and xylanases , 1993 .

[26]  E Setter,et al.  Organization and distribution of the cellulosome in Clostridium thermocellum , 1985, Journal of bacteriology.

[27]  E. Bayer,et al.  Novel oligosaccharide constituents of the cellulase complex of Bacteroides cellulosolvens. , 1992, European journal of biochemistry.

[28]  L. L. Lin,et al.  An analysis of the extracellular xylanases and cellulases of Butyrivibrio fibrisolvens H17c. , 1991, FEMS microbiology letters.

[29]  P. Dhurjati,et al.  Properties conferred on Clostridium thermocellum endoglucanase CelC by grafting the duplicated segment of endoglucanase CelD. , 1993, Protein engineering.

[30]  L. Ljungdahl,et al.  Ecology of microbial cellulose degradation , 1985 .

[31]  M. Wilchek,et al.  The use of the avidin-biotin complex as a tool in molecular biology. , 2006, Methods of biochemical analysis.

[32]  E. Bayer,et al.  Efficient cellulose solubilization by a combined cellulosome-β-glucosidase system , 1991 .

[33]  E. Bayer,et al.  The nature of the carbohydrate-peptide linkage region in glycoproteins from the cellulosomes of Clostridium thermocellum and Bacteroides cellulosolvens. , 1993, The Journal of biological chemistry.

[34]  A. Demain,et al.  Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL‐protein reveals an unusual degree of internal homology , 1993, Molecular microbiology.

[35]  E Setter,et al.  Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum , 1983, Journal of bacteriology.

[36]  D. Kilburn,et al.  Production and properties of a factor X-cellulose-binding domain fusion protein. , 1993, Protein engineering.

[37]  J. Aubert,et al.  Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein CipA and a protein possibly involved in attachment of the cellulosome to the cell surface , 1993, Journal of bacteriology.