Seamless tubular poly(glycerol sebacate) scaffolds: high-yield fabrication and potential applications.

We have previously created scaffolds composed of a biodegradable elastomer, poly(glycerol sebacate) (PGS), which are tubular, seamless, and highly porous. Here we describe two scaffold fabrication methods developed subsequently and compare the advantages of these methods to the original by examining overall yields and scaffold characteristics, such as defect frequency and severity, wall thickness homogeneity, microstructure, porosity, and mechanical properties. Scaffolds fabricated with a heat-shrinkable (HS) mandrel had higher yield, fewer defects, more homogeneous wall thickness and microstructure, and higher porosity. Improvements in yield and scaffold characteristics likely resulted from more uniform distribution of forces resulting in lower stress concentrations in the scaffolds during removal of HS mandrels. When seeded with smooth muscle cells in a bioreactor, the optimized scaffolds retained 74% of cells, which proliferated and formed a confluent cellular layer after 21 days of in vitro culture.

[1]  Debby Gawlitta,et al.  Properties of engineered vascular constructs made from collagen, fibrin, and collagen-fibrin mixtures. , 2004, Biomaterials.

[2]  D. Wise,et al.  Effect of polymer foam morphology and density on kinetics of in vitro controlled release of isoniazid from compressed foam matrices. , 1997, Journal of biomedical materials research.

[3]  Y. Ikada,et al.  First Evidence That Bone Marrow Cells Contribute to the Construction of Tissue-Engineered Vascular Autografts In Vivo , 2003, Circulation.

[4]  Jianjun Guan,et al.  Microintegrating smooth muscle cells into a biodegradable, elastomeric fiber matrix. , 2006, Biomaterials.

[5]  Chrysanthi Williams,et al.  Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents , 2006, Annals of Biomedical Engineering.

[6]  N. L'Heureux,et al.  Human tissue-engineered blood vessels for adult arterial revascularization , 2007, Nature Medicine.

[7]  David J Mooney,et al.  Salt fusion: an approach to improve pore interconnectivity within tissue engineering scaffolds. , 2002, Tissue engineering.

[8]  L A Geddes,et al.  Compliance, elastic modulus, and burst pressure of small-intestine submucosa (SIS), small-diameter vascular grafts. , 1999, Journal of biomedical materials research.

[9]  Qijin Lu,et al.  Novel porous aortic elastin and collagen scaffolds for tissue engineering. , 2004, Biomaterials.

[10]  Chao Yang,et al.  In vitro fabrication of a tissue engineered human cardiovascular patch for future use in cardiovascular surgery. , 2006, The Annals of thoracic surgery.

[11]  R. Langer,et al.  A tough biodegradable elastomer , 2002, Nature Biotechnology.

[12]  E. Chaikof,et al.  Viscoelastic and mechanical behavior of recombinant protein elastomers. , 2005, Biomaterials.

[13]  Joseph P Vacanti,et al.  Dynamic rotational seeding and cell culture system for vascular tube formation. , 2003, Tissue engineering.

[14]  Michael S Sacks,et al.  Preparation and characterization of highly porous, biodegradable polyurethane scaffolds for soft tissue applications. , 2005, Biomaterials.

[15]  P. Ma,et al.  Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. , 2001, Tissue engineering.

[16]  K. Woodhouse,et al.  Elastomeric biodegradable polyurethane blends for soft tissue applications , 2002, Journal of Biomaterials Science. Polymer Edition.

[17]  H. Matsubara,et al.  Bone Marrow Monocyte Lineage Cells Adhere on Injured Endothelium in a Monocyte Chemoattractant Protein-1–Dependent Manner and Accelerate Reendothelialization as Endothelial Progenitor Cells , 2003, Circulation research.

[18]  Jian Yang,et al.  Novel biphasic elastomeric scaffold for small-diameter blood vessel tissue engineering. , 2005, Tissue engineering.

[19]  Jonathan M. Borwein,et al.  Modular Equations and Approximations to π , 2000 .

[20]  Jin Gao,et al.  Macroporous elastomeric scaffolds with extensive micropores for soft tissue engineering. , 2006, Tissue engineering.

[21]  A. L. Wezel.  Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous Culture , 1967, Nature.

[22]  Narutoshi Hibino,et al.  Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells. , 2005, The Journal of thoracic and cardiovascular surgery.

[23]  R T Tranquillo,et al.  Fibrin as an alternative biopolymer to type-I collagen for the fabrication of a media equivalent. , 2002, Journal of biomedical materials research.

[24]  T. Yaginuma,et al.  Shear stress as an inhibitor of vascular smooth muscle cell proliferation. Role of transforming growth factor-beta 1 and tissue-type plasminogen activator. , 1997, Arteriosclerosis, thrombosis, and vascular biology.

[25]  Athanassios Sambanis,et al.  Incorporation of intact elastin scaffolds in tissue-engineered collagen-based vascular grafts. , 2004, Tissue engineering.

[26]  R Langer,et al.  Functional arteries grown in vitro. , 1999, Science.