THE EFFECTS OF ABSCISIC ACID ON GRAPE BERRY RIPENING ARE AFFECTED BY THE TIMING OF TREATMENT

*Corresponding author: marzia.giribaldi@ispa.cnr.it Aims: Grape berry is a model non-climacteric fruit, and many researchers have focused their investigations on its onset of ripening, termed veraison. This process, lasting several days, is composed by many changes, including berry softening, colouring, and sweetening. It is not synchronous for different vines, neither for different clusters on a vine, nor for different berries in a cluster. An important issue for investigations on veraison is : when does exactly this process begin? Which is the main triggering signal? In our work we tried to elucidate how abscisic acid (ABA) treatments affect the shift between green and ripe berries on Cabernet-Sauvignon, through the treatment with ABA and with inhibitors of ABA biosynthesis (fluridone) and degradation (Tetcyclacis), and how the effects of ABA changes are dependent on berry developmental stage.

[1]  C. Chervin,et al.  Influence of ethylene on sucrose accumulation in grape berry , 2006 .

[2]  Gregory M Symons,et al.  Grapes on Steroids. Brassinosteroids Are Involved in Grape Berry Ripening1 , 2005, Plant Physiology.

[3]  N. Zhang,et al.  Abscisic acid activates acid invertases in developing grape berry , 2005 .

[4]  B. Donèche,et al.  Importance of Hormonal Profile on the Onset of Ripening in Grape Berries of Vitis Vinifera L. , 2005 .

[5]  J. Roustan,et al.  Ethylene seems required for the berry development and ripening in grape, a non-climacteric fruit , 2004 .

[6]  M. Esaka,et al.  Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins , 2004 .

[7]  K. Shinozaki,et al.  A Novel Inhibitor of 9-cis-Epoxycarotenoid Dioxygenase in Abscisic Acid Biosynthesis in Higher Plants1 , 2004, Plant Physiology.

[8]  Y. Kamiya,et al.  The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′‐hydroxylases: key enzymes in ABA catabolism , 2004, The EMBO journal.

[9]  T. Gianfagna,et al.  The roles of abscisic acid and ethylene in the abscission and senescence of cocoa flowers , 1999, Plant Growth Regulation.

[10]  D. Joyce,et al.  ABA effects on ethylene production, PAL activity, anthocyanin and phenolic contents of strawberry fruit , 2004, Plant Growth Regulation.

[11]  Phuong Chung,et al.  Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan , 2003, Nature.

[12]  N. Goto-Yamamoto,et al.  Abscisic acid and 2,4-dichlorophenoxyacetic acid affect the expression of anthocyanin biosynthetic pathway genes in ‘Kyoho’ grape berries , 2003 .

[13]  J. Klein,et al.  Tetcyclacis and Abscisic Acid Differentially Affect Growth of Wheat ( Triticum aestivum L.) Seedlings Isogenic for Reduced-Height Genes , 2000 .

[14]  A. Cutler,et al.  Formation and breakdown of ABA. , 1999, Trends in plant science.

[15]  Y. Kamata,et al.  Localization of Abscisic Acid in Grape Berry by Immunohistochemical Techniques , 1999 .

[16]  M. Loewen,et al.  (+)-Abscisic acid 8'-hydroxylase is a cytochrome P450 monooxygenase , 1998, Plant physiology.

[17]  F. Mannini,et al.  The Effect of Grapevine Leafroll and Rugose Wood Sanitation on Agronomic Performance and Berry and Leaf Phenolic Content of a Nebbiolo Clone (Vitis vinifera L.) , 2018, American Journal of Enology and Viticulture.

[18]  P. Boss,et al.  Treatment of Grape Berries, a Nonclimacteric Fruit with a Synthetic Auxin, Retards Ripening and Alters the Expression of Developmentally Regulated Genes , 1997, Plant physiology.

[19]  M. Talón,et al.  Abscisic acid increases in non‐growing and paclobutrazol‐treated fruits of seedless mandarins , 1995 .

[20]  W. Hartung,et al.  The uptake and flow of C, N and ions between roots and shoots in Ricinus communis L. III. Long-distance transport of abscisic acid depending on nitrogen nutrition and salt stress , 1994 .

[21]  T. Koussa,et al.  Mise en évidence d'une relation entre les teneurs en acide abscissique des feuilles de vigne et des baies de raisin à l'époque de la véraison , 1993 .

[22]  William J. Davies,et al.  Abscisic acid : physiology and biochemistry , 1991 .

[23]  J. Kwiatkowski,et al.  Regulation of plant morphology by growth retardants: effects on phytohormone levels in soybean seedlings determined by immunoassay. , 1987, Plant physiology.

[24]  W. Rademacher,et al.  Tetcyclacis and triazole-type plant growth retardants: Their influence on the biosynthesis of gibberellins and other metabolic processes† , 1987 .

[25]  E. Weiler Plant Hormone Immunoassays Based on Monoclonal and Polyclonal Antibodies , 1986 .

[26]  D. F. Gillard,et al.  Abscisic Acid Metabolism by a Cell-free Preparation from Echinocystis lobata Liquid Endoserum. , 1976, Plant physiology.

[27]  C. R. Hale,et al.  The hormone content of ripening grape berries and the effects of growth substance treatments. , 1973, Plant physiology.

[28]  B. Sanwal,et al.  MODERN METHODS OF PLANT ANALYSIS , 1955 .