Establishment and Application of Reverse Transcription Recombinase-Aided Ampli�cation as A Rapid Detection Method for SARS-COV-2

Lele Ai Centers for Disease Control and Prevention Wei Liu chinese PLA center for disease control and prevention Fuqiang Ye Centers for Disease Control and Prevention Chenxi Ding Centers for Disease Control and Prevention Han Dai Eastern General Hospital Yifang Han Centers for Disease Control and Prevention Changqiang Zhu Centers for Disease Control and Prevention Ruichen Lv Centers for Disease Control and Prevention Zhaojiu Han Centers for Disease Control and Prevention Xiaobo Zou Jiangsu University Yong Qi Centers for Disease Control and Prevention Weilong Tan (  njcdc@163.com ) Centers for Disease Control and Prevention

[1]  B. Graham,et al.  Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation , 2020, Science.

[2]  Yuntao Wu Compensation of ACE2 Function for Possible Clinical Management of 2019-nCoV-Induced Acute Lung Injury , 2020, Virologica Sinica.

[3]  Federico M Giorgi,et al.  Genomic variance of the 2019‐nCoV coronavirus , 2020, Journal of medical virology.

[4]  E. Holmes,et al.  A new coronavirus associated with human respiratory disease in China , 2020, Nature.

[5]  Kai Zhao,et al.  A pneumonia outbreak associated with a new coronavirus of probable bat origin , 2020, Nature.

[6]  Malik Peiris,et al.  Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia , 2020, Clinical chemistry.

[7]  E. Holmes,et al.  Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding , 2020, The Lancet.

[8]  Andrea du Toit,et al.  Outbreak of a novel coronavirus , 2020, Nature Reviews Microbiology.

[9]  G. Gao,et al.  A Novel Coronavirus from Patients with Pneumonia in China, 2019 , 2020, The New England journal of medicine.

[10]  Y. Hu,et al.  Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China , 2020, The Lancet.

[11]  Z. Memish,et al.  The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health — The latest 2019 novel coronavirus outbreak in Wuhan, China , 2020, International Journal of Infectious Diseases.

[12]  C. Althaus,et al.  Pattern of early human-to-human transmission of Wuhan 2019 novel coronavirus (2019-nCoV), December 2019 to January 2020 , 2020, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[13]  Victor M Corman,et al.  Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR , 2020, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[14]  Chiyu Zhang,et al.  A Mismatch-tolerant RT-LAMP Method for Molecular Diagnosis of Highly Variable Viruses. , 2019, Bio-protocol.

[15]  Young Ki Choi,et al.  Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform , 2019, BMC Infectious Diseases.

[16]  Qin Chen,et al.  A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus. , 2019, BioTechniques.

[17]  Yi Fan,et al.  Bat Coronaviruses in China , 2019, Viruses.

[18]  Zhènglì Shí,et al.  Origin and evolution of pathogenic coronaviruses , 2018, Nature Reviews Microbiology.

[19]  F. Zeng,et al.  Development of a real time reverse transcription loop-mediated isothermal amplification method (RT-LAMP) for detection of a novel swine acute diarrhea syndrome coronavirus (SADS-CoV) , 2018, Journal of Virological Methods.

[20]  R. Hayden,et al.  Applications of Digital PCR in Clinical Microbiology , 2018 .

[21]  B. Sjögreen,et al.  Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR) , 2017, Biomolecular detection and quantification.

[22]  Z. Wan,et al.  A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses , 2016, International journal of molecular sciences.

[23]  D. Falzarano,et al.  SARS and MERS: recent insights into emerging coronaviruses , 2016, Nature Reviews Microbiology.

[24]  Lisa E. Gralinski,et al.  A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence , 2015, Nature Medicine.